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http://dx.doi.org/10.4014/jmb.1006.06032

Characterization of Two Urease-Producing and Calcifying Bacillus spp. Isolated from Cement  

Achal, Varenyam (Department of Biotechnology, Thapar University)
Mukherjee, Abhijit (Department of Biotechnology, Thapar University)
Reddy, M. Sudhakara (Department of Biotechnology, Thapar University)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.11, 2010 , pp. 1571-1576 More about this Journal
Abstract
Two bacterial strains designated as CT2 and CT5 were isolated from highly alkaline cement samples using the enrichment culture technique. On the basis of various physiological tests and 16S rRNA sequence analysis, the bacteria were identified as Bacillus species. The urease production was 575.87 U/ml and 670.71 U/ml for CT2 and CT5, respectively. Calcite constituted 27.6% and 31% of the total weight of sand samples plugged by CT2 and CT5, respectively. Scanning electron micrography analysis revealed the direct involvement of these isolates in calcite precipitation. This is the first report of the isolation and identification of Bacillus species from cement. Based on the ability of these bacteria to tolerate the extreme environment of cement, they have potential to be used in remediating the cracks and fissures in various building or concrete structures.
Keywords
Bacillus; cement; 16S rRNA; urease; calcite precipitation; crack remediation;
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1 Sharma, A., A. Pandey, Y. S. Shouche, B. Kumar, and G. Kulkarni. 2009. Characterization and identification of Geobacillus spp. isolated from Soldhar hot spring site of Garhwal Himalaya, India. J. Basic Microbiol. 49: 187-194.   DOI   ScienceOn
2 Aono, R., M. Ito, and T. Machida. 1999. Contribution of cell wall component teichuronopeptide to pH homeostasis and alkaliphily in the alkaliphile Bacillus lentus C-125. J. Bacteriol. 181: 6600-6606.
3 APHA. 1989. Standard Methods for the Examination of Water and Wastewater, 17th Ed. American Public Health Association, Washington.
4 Bachmeier, K. L., A. E. Williams, J. R. Warmington, and S. S. Bang. 2002. Urease activity in microbiologically induced calcite precipitation. J. Biotechnol. 93: 171-181.   DOI   ScienceOn
5 Bang, S. S. and V. Ramakrishnan. 2001. Microbiologically enhanced crack remediation (MECR), pp. 3-13. Proceedings of the International Symposium on Industrial Application of Microbial Genomes, Daegu, Korea.
6 Bang, S. S., J. K. Galinat, and V. Ramakrishnan. 2001. Calcite precipitation induced by polyurethane-immobilized Bacillus pasteurii. Enz. Microb. Technol. 28: 404-409.   DOI   ScienceOn
7 Boquet, E., A. Boronat, and A. Ramos-Cormenzana. 1973. Production of calcite (calcium carbonate) crystals by soil bacteria is a general phenomenon. Nature 246: 527-529.   DOI
8 Burne, R. A. and R. E. Chen. 2001. Bacterial ureases in infectious diseases. Microbes Infect. 2: 533-542.
9 Burne, R. A. and R. E. Marquis. 2000. Alkali production by oral bacteria and protection against dental caries. FEMS Microbiol. Lett. 193: 1-6.   DOI   ScienceOn
10 Achal, V., A. Mukherjee, and M. S. Reddy. 2010. Biocalcification by Sporosarcina pasteurii using corn steep liquor as nutrient source. Industr. Biotechnol. 6: 170-174.   DOI   ScienceOn
11 Achal, V., A. Mukherjee, and M. S. Reddy. 2010. Microbial concrete: A way to enhance the durability of building structures. J. Mater. Civil Eng. DOI: 10.1061/(ASCE)MT.1943-5533.0000159
12 Achal, V., A. Mukherjee, P. C. Basu, and M. S. Reddy. 2009. Lactose mother liquor as an alternative nutrient source for microbial concrete production by Sporosarcina pasteurii. J. Ind. Microbiol. Biotechnol. 36: 433-438.   DOI   ScienceOn
13 Achal, V., A. Mukherjee, P. C. Basu, and M. S. Reddy. 2009. Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production. J. Ind. Microbiol. Biotechnol. 36: 981-988.   DOI   ScienceOn
14 Morita, R. Y. 1980. Calcite precipitation by marine bacteria. Geomicrobiol. J. 2: 63-82.   DOI
15 Altschul, S. F., T. L. Madden, A. A. Schaffer, S. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.   DOI
16 Smith, N. R., R. E. Gordon, and F. E. Clark. 1952. Aerobic Spore Forming Bacteria, pp. 16. US Department of Agriculture Monograph, Washington.
17 Stocks-Fischer, S., J. K. Galinat, and S. S. Bang. 1999. Microbiological precipitation of $CaCO_{3}$. Soil Biol. Biochem. 31: 1563-1571.   DOI   ScienceOn
18 Tamura, K., J. Dudley, M. Nei, and S. Kumar. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24: 1596-1599.   DOI   ScienceOn
19 Tiano, P., L. Biagiotti, and G. Mastromei. 1999. Bacterial biomediated calcite precipitation for monumental stones conservation: Methods of evaluation. J. Microbiol. Methods 36: 138-145.
20 Tsuneda, S., J. Jung, H. Hayashi, H. Aikawa, A. Hirata, and H. Sasaki. 2003. Influence of extracellular polymers on electrokinetic properties of heterotrophic bacterial cells examined by soft particle electrophoresis theory. Colloid Surface B 29: 181-188.   DOI   ScienceOn
21 Rivadeneyra, M. A., R. Delgado, G. Delgado, A. Moral, M. R. Ferrer, and A. Ramos-Cormenzana. 1993. Precipitation of carbonate by Bacillus sp. isolated from saline soils. Geomicrobiol. J. 11: 175-184.   DOI   ScienceOn
22 Natarajan, K. R. 1995. Kinetic study of the enzyme urease from Dolichos biflorus. J. Chem. Educ. 72: 556-557.   DOI
23 Ramachandran, S. K., V. Ramakrishnan, and S. S. Bang. 2001. Remediation of concrete using microorganisms. Am. Con. Inst. Mat. J. 98: 3-9.
24 Rivadeneyra, M. A., R. Delgado, A. Moral, M. R. Ferrer, and A. Ramos-Cormenzana. 1994. Precipitation of calcium carbonate by Vibrio spp. from an inland saltern. FEMS Microbiol. Ecol. 13: 197-204.   DOI   ScienceOn
25 McConnaughey, T. A. and F. F. Whelan. 1997. Calcification generates protons for nutrient and bicarbonate uptake. Earth Sci. Rev. 42: 95-117.   DOI   ScienceOn
26 Kawaguchi, T. and A. W. Decho. 2002. A laboratory investigation of cyanobacterial extracellular polymeric secretions (EPS) in influencing $CaCO_{3}$ polymorphism. J. Cryst. Growth 240: 230-235.   DOI   ScienceOn
27 Krishna, P., A. Arora, and M. S. Reddy. 2008. An alkaliphilic and xylanolytic strain of actinomycetes, Kocuria sp. RM1, isolated from extremely alkaline bauxite residue sites. World J. Microbiol. Biotechnol. 24: 3079-3085.   DOI   ScienceOn
28 Krulwich, T. A. and A. A. Guffanti. 1989. Alkalophilic bacteria. Annu. Rev. Microbiol. 43: 435-463.   DOI   ScienceOn
29 Mobley, H. L. T. and R. P. Hausinger. 1989. Microbial ureases: Significance, regulation and molecular characterisation. Microbiol. Rev. 53: 85-108.
30 Chafetz, H. S. and R. L. Folk. 1984. Travertines: Depositional morphology and the bacterially constructed constituents. J. Sed. Petrol. 54: 289-316.
31 Cole, J. R., B. Chai, T. L. Marsh, R. J. Farris, Q. Wang, S. A. Kulam, et al. 2003. The ribosomal database project (RDP-II): Previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res. 31: 442- 443.   DOI   ScienceOn
32 Friedman, L. E., B. N. de Passerini Rossi, M. T. Messina, and M. A. Franco. 2001. Phenotype evaluation of Bordetella bronchiseptica cultures by urease activity and Congo red affinity. Lett. Appl. Microbiol. 33: 285-290.   DOI   ScienceOn
33 Fukumoto, J., T. Yamamoto, and D. Tsuru. 1971. Process for producing detergent resisting alkaline protease. Canadian Patent 910: 214.
34 Gollapudi, U. K., C. L. Knutson, S. S. Bang, and M. R. Islam. 1995. A new method for controlling leaching through permeable channels. Chemosphere 30: 695-705.   DOI   ScienceOn
35 Gordon, R. E., W. C. Haynes, and C. H. Pang. 1973. The Genus Bacillus, pp. 427. US Department of Agriculture Handbook, Washington.
36 Kantzas, A., F. G. Ferris, L. Stehmeier, D. F. Marentette, K. N. Jha, and F. M. Mourits. 1992. A novel method of sand consolidation through bacteriogenic mineral plugging (CIM 92- 46), pp. 1-15. Proceedings of the CIM Annual Technical Conference, Vol. 2. Petroleum Society of CIM, Calgary, Canada.