Browse > Article

Isolation and Characterization of the Eicosapentaenoic Acid Biosynthesis Gene Cluster from Shewanella sp. BR-2  

Lee, Su-Jin (Molecular Bioprocess Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Seo, Pil-Soo (Molecular Bioprocess Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kim, Chul-Ho (Molecular Bioprocess Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kwon, Oh-Suk (Omics and Integration Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Hur, Byung-Ki (Department of Biological Engineering and Institute of Biotechnological Industry, Inha University)
Seo, Jeong-Woo (Molecular Bioprocess Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Publication Information
Journal of Microbiology and Biotechnology / v.19, no.9, 2009 , pp. 881-887 More about this Journal
Abstract
Forty-four eicosapentaenoic acid (EPA)-producing microbial strains were isolated from the intestines of marine fishes. Among them, one strain showing a maximum level of EPA (4.78% of total fatty acids) was identified as Shewanella sp. BR-2 on the basis of its 168 rRNA sequence. The EPA content reached a maximum level during the mid-exponential phase of cell growth, and gradually decreased with further growth of the cells. A cosmid DNA including the EPA biosynthesis gene cluster consisting of pfaA-E was isolated from a cosmid library of genomic DNA of Shewanella sp. BR-2, named pCosEPA-BR2. An E. coli clone harboring pCosEPA-BR2 produced EPA at a maximum level of 7.5% of total fatty acids, confirming the EPA biosynthesis activity of the cloned gene cluster.
Keywords
Eicosapentaenoic acid; Shewanella sp. BR-2; PKS-like module; biosynthesis gene cluster;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
1 Angerer, P. and C. V. Schachy. 2000. n-3 Polyunsaturated fatty acids and the cardiovascular system. Curr. Opin. Clin. Nutr. Metab. Care 3: 439-445   DOI   ScienceOn
2 Braden, L. M. and K. K. Caroll. 1986. Dietary polyunsaurated fat in relation to mammary carcinogenesis in rats. Lipids 21: 285-288   DOI   ScienceOn
3 Lauritzen, L., H. S. Hansen, M. H. Jorgensen, and K. F. Michaelsen. 2001. The essentiality of long chain n-3 fatty acids in relation to development and function of the brain and retina. Prog. Lipid Res. 40: 1-94   DOI   ScienceOn
4 Okuyama, H., Y. Orikasa, T. Nishida, K. Watanabe, and N. Norita. 2007. Bacterial genes responsible for the biosynthesis of eicosapentaenoic and docosaphexenoic acids and their heterologous expression. Appl. Environ. Microbiol. 73: 665-670   DOI   ScienceOn
5 Orikasa, Y., A. Yamada, R. Yu, Y. Ito, T. Nishida, I. Yumoto, K. Watanabe, and H. Okuyama. 2004. Characterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. strain SCRC-2738. Cell Mol. Biol. 50: 625-630
6 Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680   DOI   ScienceOn
7 Tresguerres, J. A. F., V. Lahera, and M. V. Cachofeiro. 1989. Dietary essential polyunsaturated fatty acids and blood pressure. News Physiol. Sci. 4: 64-68
8 Wallis, J. G., J. L. Watts, and J. Browse. 2002. Polyunsaturated fatty acid synthesis: What will they think of next? Trends Biochem. Sci. 27: 467-473   DOI   PUBMED   ScienceOn
9 Chung, T. H., S. J. Lee, H. J. Oh, G. J. Kim, and B. K. Hur. 2005. Cloning of a $\Delta$5 desaturase from Thraustochytrium sp. 26185 and functional expression in Pichia pastoris. Biotechnol. Bioprocess Eng. 20: 93-99
10 Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111-120   DOI   PUBMED
11 Abbey, M., P. Clifton, M. Kestin, B. Belling, and P. Nestel. 1990. Effect of fish oil on lipoproteins, lecithin:cholesterol acyltransferase, and lipid transfer protein activity in humans. Arteriosclerosis 10: 85-94   DOI   PUBMED
12 Metz, J. G., P. Roessler, D. Facciotti, C. Levering, F. Dittrich, M. Lassner, et al. 2001. Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science 293: 290-293   DOI   PUBMED   ScienceOn
13 Kaulmann, U. and C. Hertweck. 2002. Biosynthesis of polyunsaturated fatty acids by polyketide synthases. Angew. Chem. Int. Ed. 41: 1866-1869   DOI   ScienceOn
14 Yazawa, K. 1996. Production of eicosapentaenoic acid from marine bacteria. Lipids 31: S297-S300   DOI   PUBMED
15 Sauer, L. A., R. T. Dauchy, and D. E. Blask. 2001. Polyunsaturated fatty acids, melatonin, and cancer prevention. Biochem. Pharmacol. 61: 1455-1462   DOI   PUBMED   ScienceOn
16 Qiu, X. 2003. Biosynthesis of docosahexaenoic acid (DHA, 22:6-4, 7,10,13,16,19): Two distinct pathways. Prostaglandins Leukot. Essen. Fatty Acids 68: 181-186   DOI   ScienceOn
17 Trautwein, E. A. 2001. n-3 Fatty acids - physiological and technical aspects for their role in food. Eur. J. Lipid Sci. Technol. 103: 45-55   DOI   ScienceOn
18 Jiang, H., R. Zirkle, J. G. Metz, L. Braun, L. Richter, S. G. Van Lanen, and B. Shen. 2008. The role of tandem acyl carrier protein domains in polyunsaturated fatty acid biosynthesis. J. Am. Chem. Soc. 130: 6336-6337   DOI   ScienceOn
19 Lee, S. J., Y. S. Jeong, D. U. Kim, J.-W. Seo, and B. K. Hur. 2006. Eicosapentaenoic acid (EPA) biosynthetic gene cluster of Shewanella oneidensis MR-1: Cloning, heterologous expression, and effect of temperature and glucose on the production of EPA in Escherichia coli. Biotech. Bioprocess Eng. 11: 510-515   DOI   ScienceOn
20 Medina, A. R., E. M. Grima, A. G. Gimenez, and M. J. I. Gonzalez. 1998. Downstream processing of algal polyunsaturated fatty acids. Biotechnol. Adv. 16: 517-580   DOI   ScienceOn
21 Fiocchi, A., M. Sala, P. Signoroni, G. Banderal, C. Agostoul, and E. Riva. 1994. The efficiency and safety of $\gamma$-linolenic acid in the treatment of infantile atopic dermatitis. J. Int. Med. Res. 22: 2-32
22 Tamura, K., J. Dudley, M. Nei, and S. Kumar. 2007. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596-1599   DOI   ScienceOn