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http://dx.doi.org/10.4014/jmb.0901.057

Enhanced Delivery of siRNA Complexes by Sonoporation in Transgenic Rice Cell Suspension Cultures  

Cheon, Su-Hwan (Department of Biological Engineering, Inha University)
Lee, Kyoung-Hoon (Department of Biological Engineering, Inha University)
Kwon, Jun-Young (Department of Biological Engineering, Inha University)
Choi, Sung-Hun (Department of Biological Engineering, Inha University)
Song, Mi-Na (Department of Biological Engineering, Inha University)
Kim, Dong-II (Department of Biological Engineering, Inha University)
Publication Information
Journal of Microbiology and Biotechnology / v.19, no.8, 2009 , pp. 781-786 More about this Journal
Abstract
Small interfering synthetic double-stranded RNA (siRNA) was applied to suppress the expression of the human cytotoxic-T-Iymphocyte antigen 4-immunoglobulin (hCTLA4Ig) gene transformed in transgenic rice cell cultures. The sequence of the 21-nucleotide siRNA was deliberately designed and synthesized with overhangs to inactivate the expression of hCTLA4Ig. The chemically synthesized siRNA duplex was combined with polyethyleneimine (PEl) at a mass ratio of 1:10 (0.33 ${\mu}g$ siRNA:3.3 ${\mu}g$ PEl) to produce complexes. The siRNA complexes (siRNA+PEI) were labeled with Cy3 in order to subsequently confirm the delivery by fluorescent microscopy. In addition, the cells were treated with sonoporation at 40 kHz and 419W for 90 s to improve the delivery. The siRNA complexes alone inhibited the expression of hCTLA4Ig to 45% compared with control. The siRNA complexes delivered with sonoporation downregulated the production of hCTLA4Ig to 73%. Therefore, we concluded that the delivery of siRNA complexes into plant cells could be enhanced successfully by sonoporation.
Keywords
Polyethyleneimine; transgenic plant cell cultures; siRNA; sonoporation; hCTLA4Ig;
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