Novel Modification of Growth Medium Enables Efficient E. coli Expression and Simple Purification of an Endotoxin-Free Recombinant Murine Hsp70 Protein
![]() |
Zachova, Katerinat
(Department of Immunology, Faculty of Medicine and Dentistry, Palacky University in Olomouc)
Krupka, Michal (Department of Immunology, Faculty of Medicine and Dentistry, Palacky University in Olomouc) Chamrad, Ivo (Department of Biochemistry, Faculty of Science, Palacky University in Olomouc) Belakova, Jana (Department of Immunology, Faculty of Medicine and Dentistry, Palacky University in Olomouc) Horynova, Milada (Department of Immunology, Faculty of Medicine and Dentistry, Palacky University in Olomouc) Weigl, Evzen (Department of Immunology, Faculty of Medicine and Dentistry, Palacky University in Olomouc) Sebela, Marek (Department of Biochemistry, Faculty of Science, Palacky University in Olomouc) Raska, Milan (Department of Immunology, Faculty of Medicine and Dentistry, Palacky University in Olomouc) |
1 | Aida, Y. and M. J. Pabst. 1990. Removal of endotoxin from protein solutions by phase separation using Triton X-114. J. Immunol. Methods 132: 191-195 DOI ScienceOn |
2 | Baykov, A. A., O. A. Evtushenko, and S. M. Avaeva. 1988. A malachite green procedure for orthophosphate determination and its use in alkaline phosphatase-based enzyme immunoassay. Anal. Biochem. 171: 266-270 DOI ScienceOn |
3 | Benaroudj, N., B. Fang, F. Triniolles, C. Ghelis, and M. M. Ladjimi. 1994. Overexpression in Escherichia coli, purification and characterization of the molecular chaperone HSC70. Eur. J. Biochem. 221: 121-128 DOI ScienceOn |
4 | Geladopoulos, T. P., T. G. Sotiroudis, and A. E. Evangelopoulos. 1991. A malachite green colorimetric assay for protein phosphatase activity. Anal. Biochem. 192: 112-116 DOI ScienceOn |
5 | Nadeau, K., M. A. Sullivan, M. Bradley, D. M. Engman, and C. T. Walsh. 1992. 83-Kilodalton heat shock proteins of trypanosomes are potent peptide-stimulated ATPases. Protein Sci. 1: 970-979 DOI ScienceOn |
6 | Stosova, T., M. Sebela, P. Rehulka, O. Sedo, J. Havlis, and Z. Zdrahal. 2008. Evaluation of the possible proteomic application of trypsin from Streptomyces griseus. Anal. Biochem. 376: 94-102 DOI ScienceOn |
7 | Wieland, A., M. Denzel, E. Schmidt, S. Kochanek, F. Kreppel, J. Reimann, and R. Schirmbeck. 2008. Recombinant complexes of antigen with stress proteins are potent CD8 T-cell-stimulating immunogens. J. Mol. Med. 86: 1067-1079 DOI ScienceOn |
8 | Raska, M., J. Belakova, N. K. Wudattu, L. Kafkova, K. Ruzickova, M. Sebestova, Z. Kolar, and E. Weigl. 2005. Comparison of protective effect of protein and DNA vaccines hsp90 in murine model of systemic candidiasis. Folia Microbiol. (Praha) 50: 77-82 DOI ScienceOn |
9 | Waugh, D. F. 1954. Protein-protein interactions. Adv. Protein Chem. 9: 325-437 DOI PUBMED ScienceOn |
10 | Sebela, M., T. Stosova, J. Havlis, N. Wielsch, H. Thomas, Z. Zdrahal, and A. Shevchenko. 2006. Thermostable trypsin conjugates for high-throughput proteomics: Synthesis and performance evaluation. Proteomics 6: 2959-2963 DOI ScienceOn |
11 | Valentinis, B., A. Capobianco, F. Esposito, A. Bianchi, P. Rovere-Querini, A. A. Manfredi, and C. Traversari. 2008. Human recombinant heat shock protein 70 affects the maturation pathways of dendritic cells in vitro and has an in vivo adjuvant activity. J. Leukoc. Biol. 84: 199-206 DOI ScienceOn |
12 | Vanhove, M., S. Houba, J. b1motte-Brasseur, and J. M. Frere. 1995. Probing the determinants of protein stability: Comparison of class A beta-lactamases. Biochem. J. 308(Pt 3): 859-864 DOI ScienceOn |
13 | Udono, H., T. Saito, M. Ogawa, and Y. Yui. 2004. Hsp-antigen fusion and their use for immunization. Methods 32: 21-24 DOI ScienceOn |
14 | Nadeau, K., A. Das, and C. T. Walsh. 1993. Hsp90 chaperonins possess ATPase activity and bind heat shock transcription factors and peptidyl prolyl isomerases. J. Biol. Chem. 268: 1479-1487 PUBMED |
15 | Bausinger, H., D. Lipsker, U. Ziylan, S. Manie, J. P. Briand, J. P. Cazenave, et al. 2002. Endotoxin-free heat-shock protein 70 fails to induce APC activation. Eur. J. Immunol 32: 3708-3713 DOI ScienceOn |
16 | Menoret, A. 2004. Purification of recombinant and endogenous HSP70s. Methods 32: 7-12 DOI PUBMED ScienceOn |
17 | Hauser, H. and S. Y. Chen. 2003. Augmentation of DNA vaccine potency through secretory heat shock protein-mediated antigen targeting. Methods 31: 225-231 DOI ScienceOn |
18 | Malyala, P. and M. Singh. 2008. Endotoxin limits in formulations for preclinical research. J. Pharm. Sci. 97: 2041-2044 DOI ScienceOn |
19 | Lipsker, D., U. Ziylan, D. Spehner, F. Proamer, H. Bausinger, P. Jeannin, et al. 2002. Heat shock proteins 70 and 60 share common receptors which are expressed on human monocyte-derived but not epidermal dendritic cells. Eur. J. Immunol. 32: 322-332 DOI ScienceOn |
20 | Raska, M., J. Belakova, M. Horynova, M. Krupka, J. Novotny, M. Sebestova, and E. Weigl. 2008. Systemic and mucosal immunization with Candida albicans hsp90 elicits hsp90-specific humoral response in vaginal mucosa which is further enhanced during experimental vaginal candidiasis. Med. Mycol. 46: 411-420 DOI ScienceOn |
21 | Suzue, K., X. Zhou, H. N. Eisen, and R. A. Young. 1997. Heat shock fusion proteins as vehicles for antigen delivery into the major histocompatibility complex class I presentation pathway. Proc. Natl. Acad. Sci. U.S.A. 94: 13146-13151 DOI ScienceOn |
22 | Ye, Z. and Y. H. Gan. 2007. Flagellin contamination of recombinant heat shock protein 70 is responsible for its activity on T cells. J. Biol. Chem. 282: 4479-4484 DOI ScienceOn |
23 | Hauser, H., L. Shen, Q. L. Gu, S. Krueger, and S. Y. Chen. 2004. Secretory heat-shock protein as a dendritic cell-targeting molecule: A new strategy to enhance the potency of genetic vaccines. Gene Ther. 11: 924-932 DOI ScienceOn |
24 | Olson, C. L., K. C. Nadeau, M. A. Sullivan, A. G. Winquist, J. E. Donelson, C. T. Walsh, and D. M. Engman. 1994. Molecular and biochemical comparison of the 70-kDa heat shock proteins of Trypanosoma cruzi. J. Biol. Chem. 269: 3868-3874 PUBMED |
![]() |