Browse > Article
http://dx.doi.org/10.4014/jmb.0811.608

Production of Flavonoid O-Glucoside Using Sucrose Synthase and Flavonoid O-Glucosyltransferase Fusion Protein  

Son, Mi-Hyel (Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University)
Kim, Bong-Gyu (Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University)
Kim, Dae-Hwan (Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Gyeonggido Institute of Health and Environment)
Jin, Mi-Rim (Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University)
Kim, Kwang-Pyo (Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University)
Ahn, Joong-Hoon (Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.19, no.7, 2009 , pp. 709-712 More about this Journal
Abstract
Enzymatic glucosylation with glycosyltransferases can be used to regulate the water solubility of aglycone. The drawback of this process is the demand of UDP-glucose as a sugar donor. We made an in-frame fusion of the flavonoid O-glucosyltransferase (OsUGT-3) and sucrose synthase (AtSUS) genes. The resulting fusion protein, OsUGT3-AtSUS, was expressed in E. coli and purified. When sucrose and UDP were supplied, the fusion protein was able to convert quercetin into quercetin O-glucoside without the addition of UDP-glucose. In addition, UDP-glucose was recycled when sucrose was added to the reaction mixture. This fusion protein is useful for the enzymatic production of flavonoid O-glucosides.
Keywords
Flavonoid; glucosyltransferase; sucrose synthase; UDP-glucose;
Citations & Related Records

Times Cited By Web Of Science : 1  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Boudet, A.-M. 2007. Evolution and current status of research in phenolic compounds. Phytochemistry 68: 2722-2735   DOI   ScienceOn
2 Gachon, C. M. M., M. Langlois-Meurinne, and P. Saindrenan. 2005. Plant secondary metabolism glycosyltransferases: The emerging functional analysis. Trends Plant Sci. 10: 542-549   DOI   ScienceOn
3 Halliwell, B. 2007. Dietary polyphenols: Good, bad, or indifferent for your health? Cardiovasc. Res. 73: 341-347   DOI   PUBMED   ScienceOn
4 Lim, E.-K., D. A. Ashford, B. Hou, R. G. Jackson, and D. J. Bowles. 2004. Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides. Biotech. Bioeng. 87: 623-631   DOI   ScienceOn
5 Winkel-Shirley, B. 2001. Flavonoid biosynthesis; A colorful model for genetics, biochemistry, cell biology and biotechnology. Plant Physiol. 126: 485-493   DOI   PUBMED   ScienceOn
6 Breton, C. and A. Imberty. 1999. Structure/function studies of glycosyltransferases, Curr. Opin. Struct. Biol. 9: 563-571   DOI   ScienceOn
7 Esp$\acute{i}$n, J. C., M. T. Garc$\acute{i}$a-Conesa, and F. A. Tom$\acute{a}$s-Barberán. 2007. Nutraceuticals: Facts and fiction. Phytochemistry. 68: 2986-3008   DOI   ScienceOn
8 Bowles, D., J. Lsayenkova, E.-K. Lim, and B. Poppenberger. 2005. Glycosyltransferases: Managers of small molecules. Curr. Opin. Plant Biol. 8: 254-263   DOI   ScienceOn
9 Ko, J. H., B. G. Kim, J. H. Kim, H. Kim, C. E. Lim, J. Lim, C. Lee, Y. Lim, and J.-H. Ahn. 2008. Four glucosyltransferases from rice: cDNA cloning, expression, and characterization. J. Plant Physiol. 165: 435-444   DOI   ScienceOn
10 Masada, S., Y. Kawase, M. Nagotoshi, Y. Oguchi, K. Terasaka, and H. Mizukami. 2007. An efficient chemoenzymatic production of small molecule glucosides with in situ UDP-glucose recycling. FEBS Lett. 581: 2562-2566   DOI   ScienceOn
11 Rupprath, C., T. Schumacher, and L. Elling. 2005. Nucleotide deoxysugars: Essential tools for the glycosylation engineering of novel bioactive compounds. Curr. Med. Chem. 12: 1637-1675   DOI   ScienceOn
12 Jones, P. and T. Vogt. 2001. Glycosyltransferases in secondary plant metabolism tranquilizers and stimulant controllers. Planta 213: 164-174   DOI   ScienceOn
13 M$\acute{e}$ndez, C. and J. A. Salas. 2001. Altering the glycosylation pattern of bioactive compounds. Trends Biotechnol. 19: 449-456   DOI   ScienceOn
14 Kim, J. H., K. H. Shin, J. H. Ko, and J.-H. Ahn. 2006. Glucosylation of flavonols with Escherichia coli cells expressing a glucosyltransferase from rice (Oryza sativa). J. Biosci. Bioeng. 102: 135-137   DOI   ScienceOn
15 Tahara, S. A. 2005. Journey of twenty-five years through the ecological biochemistry of flavonoids. Biosci. Biotechnol. Biochem. 71: 1387-1404   DOI   ScienceOn