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http://dx.doi.org/10.4014/jmb.0811.644

Isolation of 1',3'-Dilinolenoyl-2'-Linoleoylglycerol with Tyrosinase Inhibitory Activity from Flammulina velutipes  

Jang, Se-Gul (Department of Bioindustry and Technology, Sangji University)
Jeon, Kyung-Su (Department of Bioindustry and Technology, Sangji University)
Lee, Eun-Hee (Department of Bioindustry and Technology, Sangji University)
Kong, Won-Sik (Applied Microbiology Division, National Institute of Agricultural Science and Technology, R.D.A.)
Cho, Jae-Yong (Department of Bioindustry and Technology, Sangji University, Division of Animal Science and Biotechnology, Sangji University)
Publication Information
Journal of Microbiology and Biotechnology / v.19, no.7, 2009 , pp. 681-684 More about this Journal
Abstract
This study was carried out to evaluate the inhibitory effect of Flammulina velutipes extracts on tyrosinase activity and to identify its biologically active component. The ethyl acetate and n-butanol extracts showed potent tyrosinase inhibitory activities. Subsequently, fractions of the n-butanol extract showed only a partial tyrosinase inhibitory activity. The most active compound of tyrosinase inhibitory activity was identified from the ethyl acetate extract as 1',3'-dilinolenoyl-2'-linoleoylglycerol (LnLLn) by comparing its mass, $^1H-$, and $^{13}C-NMR$spectral data with those previously reported in the literature. LnLLn showed tyrosinase inhibitory activity with an $IC_{50}$value of 16.1 ${\mu}g/ml$. These results suggest that the ethyl acetate extract of F. velutipes could be applicable for the development of a new whitening agent.
Keywords
1',3'-Dilinolenoyl-2'-linoleoylglycerol (LnLLn); Flammulina velutipes; tyrosinase inhibitory activity; whitening effect;
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