Browse > Article
http://dx.doi.org/10.4014/jmb.0811.603.

Suppression of Metastasis of Human Breast Cancer Cells by Chitosan Oligosaccharides  

Nam, Kyung-Soo (Department of Pharmacology, College of Medicine, Dongguk University)
Shon, Yun-Hee (Intractable Disease Research Center, Dongguk University)
Publication Information
Journal of Microbiology and Biotechnology / v.19, no.6, 2009 , pp. 629-633 More about this Journal
Abstract
The present study investigated the antimetastatic property of chitosan oligosaccharides (COS) by evaluating motility, invasion, and the amount and activity of MMP-9 in MDA-MB-231 human breast carcinoma cells. Treatment of MDA-MB-231 cells with increasing concentrations of COS led to a concentration-dependent decrease in cell migration. COS significantly inhibited the invasion of MDA-MB-231 cells through a Matrigel-coated membrane. The treatment of MDA-MB-231 cells with COS reduced the amounts of secreted MMP-9. The activity and amount of MMP-9 protein in MDA-MB-231 cells were decreased by treatment with COS and occurred in a concentration-dependent manner. Our data indicated that COS can serve as a potential novel therapeutic candidate for the treatment of metastatic breast cancer.
Keywords
Breast cancer; chitosan oligosaccharides; invasion; metastasis; matrix metalloproteinase;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 7  (Related Records In Web of Science)
연도 인용수 순위
1 Jeon, C. 2005. Mercury ion removal using a packed-bed column with granular aminated chitosan. J. Microbiol. Biotechnol. 15:497-501   과학기술학회마을   ScienceOn
2 Jones, J. L., J. A. Shaw, J. H. Pringle, and R. A. Walker. 2003. Primary breast myoepithelial cells exert an invasion-suppressor effect on breast cancer cells via paracrine down-regulation of MMP expression in fibroblasts and tumour cells. J. Pathol. 201:562-572   DOI   ScienceOn
3 Khasigov, P. Z., O. V. Podobed, T. S. Gracheva, K. D. Salbiev, S. V. Grachev, and T. T. Berezov. 2003. Role of matrix metalloproteinases and their inhibitors in tumor invasion and metastasis. Biochemistry (Mosc.) 68: 711-717   DOI   ScienceOn
4 Nam, K. S., M. K. Kim, and Y. H. Shon. 2007. Inhibition of proinflammatory cytokine-induced invasiveness of HT-29 cells by chitosan oligosaccharide. J. Microbiol. Biotechnol. 17:2042-2045   과학기술학회마을   PUBMED   ScienceOn
5 Sreenath, T., L. M. Matrisian, W. Stetler-Stevenson, S. Gattoni-Celli, and R. O. Pozzatti. 1992. Expression of matrix metalloproteinases genes in transformed rat cell lines of high and low metastatic potential. Cancer Res. 52: 4942-4947   PUBMED   ScienceOn
6 Yilmaz, M., G. Christofori, and F. Lehembre. 2007. Distinct mechanisms of tumor invasion and metastasis. Trends Mol. Med. 13: 535-541   DOI   ScienceOn
7 Bachmeier, B. E., A. G. Nerlich, R. Lichtinghagen, and C. P. Sommerhoff. 2001. Matrix metalloproteinases (MMPs) in breast cancer cell lines of different tumorigenicity. Anticancer Res. 21:3821-3828   PUBMED
8 Nishimura, K., S. Nishimura, N. Nishi, I. Saiki, S. Tokura, and I. Azuma. 1984. Immunological activity of chitin and its derivatives. Vaccine 2:93-99   DOI   ScienceOn
9 Cho, E. J., Md. A. Rahman, S. W. Kim, Y. M. Baek, H. J. Hwang, J. Y. Oh, H. S. Hwang, S. H. Lee, and J. W. Yun. 2008. Chitosan oligosaccharides inhibit adipogenesis in 3T3-L1 adipocytes. J. Microbiol. Biotechnol. 18: 80-87   PUBMED
10 Gonzalez, L. O., I. Pidal, S. Junquera, M. D. Corte, J. Vazquez, J. C. Rodriguez, et al. 2007. Overexpression of matrix metalloproteinases and their inhibitors in mononuclear inflammatory cells in breast cancer correlates with metastasis-relapse. Br. J. Cancer 97:957-963   DOI   PUBMED
11 Yu, A. E., R. E. Hewitt, D. E. Kleiner, and W. G. Stetler-Stevenson. 1996. Molecular regulation of cellular invasion - role of gelatinase A and TIMP-2. Biochem. Cell Biol. 74: 823-831   DOI   ScienceOn
12 Aye, M. M., C. Ma, H. Lin, K. A. Bower, R. C. Wiggins, and J. Luo. 2004. Ethanol-induced in vitro invasion of breast cancer cells: The contribution of MMP-2 by fibroblasts. Int. J. Cancer 112: 738-746   DOI   ScienceOn
13 Chen, J. and L. U. Thompson. 2003. Lignans and tamoxifen, alone or in combination, reduce human breast cancer cell adhesion, invasion and migration in vitro. Breast Cancer Res. Treat. 80: 163-170   DOI   ScienceOn
14 Friedel, G., U. Pastorino, R. J. Ginsberg, P. Goldstraw, M. Johnston, H. Pass, J. B. Putnam, and H. Toomes. 2002. Results of lung metastasectomy from breast cancer: Prognostic criteria on the basis of 467 cases of the International Registry of Lung Metastases. Eur. J. Cardiothorac. Surg. 22: 335-344   DOI   ScienceOn
15 Liotta, L. A., K. Tryggvason, S. Garbisa, I. Hart, C. M. Foltz, and S. Shafie. 1980. Metastatic potential correlates with enzymatic degradation of basement membrane collagen. Nature 284: 67-68   DOI   ScienceOn
16 Suzuki, K., T. Mikami, Y. Okawa, A. Tokoro, S. Suzuki, and M. Suzuki. 1986. Antitumor effect of hexa-N-acetylchitohexaose and chitohexaose. Carbohydr. Res. 151: 403-408   DOI   ScienceOn
17 Stetler-Stevenson, W. G., S. Aznavoorian, and L. Liotta. 1993. Tumor cell interactions with the extracellular matrix during invasion and metastasis. Annu. Rev. Cell Biol. 9: 541-573   DOI   ScienceOn
18 Garibyan, L., C. Lenahan, and S. Pories. 2004. Metalloproteinases:Promising tumor markers for breast cancer management. Curr. Surg. 61: 255-259   DOI   ScienceOn
19 Nam, K. S., M. K. Kim, and Y. H. Shon. 2007. Chemopreventive effect of chitosan oligosaccharide against colon carcinogenesis. J. Microbiol. Biotechnol. 17: 1546-1549   과학기술학회마을   PUBMED   ScienceOn
20 Shin, W. S., J. C. Kil, and G. M. Park. 2006. Antiprotozoal activity of deacetylated chitosan oligosaccharide (dp 2-8) on Trichomonas vaginalis. J. Microbiol. Biotechnol. 16: 1984-1989   과학기술학회마을   ScienceOn