[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.0808.499

Production of Acrylic Acid from Acrylonitrile by Immobilization of Arthrobacter nitroguajacolicus ZJUTB06-99

Shen, Mei (Institute of Bioengineering, Zhejiang University of Technology)
Zheng, Yu-Guo (Institute of Bioengineering, Zhejiang University of Technology)
Liu, Zhi-Qiang (Institute of Bioengineering, Zhejiang University of Technology)
Shen, Yin-Chu (Institute of Bioengineering, Zhejiang University of Technology)

Publication Information

Journal of Microbiology and Biotechnology / v.19, no.6, 2009 , pp. 582-587 More about this Journal

Abstract

Immobilized cells of Arthrohacter nitroguajacolicus ZJUTB06-99 capable of producing nitrilase were used for biotransformation of acrylonitrile to acrylic acid. Six different entrapment matrixes were chosen to search for a suitable support in terms of nitrilase activity. Ca-alginate proved to be more advantageous over other counterparts in improvement of the biocatalyst activity and bead mechanical strength. The effects of sodium alginate concentration, $CaCl_2$ concentration, bead diameter, and ratio by weight of cells to alginate, on biosynthesis of acrylic acid by immobilized cells were investigated. Maximum activity was obtained under the conditions of 1.5% sodium alginate concentration, 3.0% $CaCl_2$ concentration, and 2-mm bead size. The beads coated with 0.10% polyethylenimine (PEI) and 0.75% glutaraldehyde (GA) could tolerate more phosphate and decrease leakage amounts of cells from the gel. The beads treated with PEI/GA could be reused up to 20 batches without obvious decrease in activities, which increased about 100% compared with the untreated beads with a longevity of 11 batches.

Keywords

Acrylic acid; acrylonitrile; Arthrobacter nitroguajacolicus; nitrilase; calcium alginate; polyethylenimine;

Citations & Related Records

Times Cited By KSCI : 2 (Citation Analysis)
Times Cited By Web Of Science : 1 (Related Records In Web of Science)

Reference
Cited By KSCI

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