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http://dx.doi.org/10.4014/jmb.0809.541

Study on Immunostimulating Activity of Macrophage Treated with Purified Polysaccharides from Liquid Culture and Fruiting Body of Lentinus edodes  

Lee, Hee-Hwan (Department of Bioengineering and Technology, Kangwon National University)
Lee, Jong-Seok (Department of Bioengineering and Technology, Kangwon National University)
Cho, Jae-Youl (School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University)
Kim, Young-Eon (Korea Food Research Institute)
Hong, Eock-Kee (Department of Bioengineering and Technology, Kangwon National University)
Publication Information
Journal of Microbiology and Biotechnology / v.19, no.6, 2009 , pp. 566-572 More about this Journal
Abstract
Lentinus edodes is a well-known edible and medicinal mushroom used in Oriental cultures. Recently, L. edodes has attracted a lot of attention owing to its antifungal activity, antibacterial activity, antiviral activity, hepatoprotective effect, antitumor activities, and immunomodulatory and cytotoxic effects. In this study, the water-soluble crude polysaccharides, CPF and CPB, which were obtained from the fruiting body and culture cell-free broth of L. edodes by hot-water extraction and ethanol precipitation, were fractionated by DEAE cellulose and Sepharose CL-6B column chromatography, resulting in six polysaccharide fractions, CPFN-G-I, CPFN-G-II, CPFN-G-III, CPFA-G, CPBN-G, and CPBA-G Among these fractions, CPFN-G-I, CPBN-G, and CPBA-G were shown to stimulate the functional activation of macrophages including NO production, cytokine expression, and phagocytosis.
Keywords
Lentinus edodes; immunostimulating polysaccharide; mushroom-derived$\beta$-glucan; macrophage activation; phagocytosis;
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Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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