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http://dx.doi.org/10.4014/jmb.0806.374

Hepatoprotective and Curative Properties of Kombucha Tea Against Carbon Tetrachloride-Induced Toxicity  

Murugesan, G.S. (Microbial Biotechnology Division, Department of Biotechnology, Bharathiar University)
Sathishkumar, M. (Division of Biotechnology, Department of Food Science and Technology, Institute of Agricultural Science and Technology, Chonbuk National University)
Jayabalan, R. (Microbial Biotechnology Division, Department of Biotechnology, Bharathiar University)
Binupriya, A.R. (Division of Biotechnology, Department of Food Science and Technology, Institute of Agricultural Science and Technology, Chonbuk National University)
Swaminathan, K. (Microbial Biotechnology Division, Department of Biotechnology, Bharathiar University)
Yun, S.E. (Division of Biotechnology, Department of Food Science and Technology, Institute of Agricultural Science and Technology, Chonbuk National University)
Publication Information
Journal of Microbiology and Biotechnology / v.19, no.4, 2009 , pp. 397-402 More about this Journal
Abstract
Kombucha tea(KT) is sugared black tea fermented with a symbiotic culture of acetic acid bacteria and yeasts, which is said to be tea fungus. KT is claimed to have various beneficial effects on human health, but there is very little scientific evidence available in the literature. In the present study, KT along with black tea(BT) and black tea manufactured with tea fungus enzymes(enzyme-processed tea, ET) were evaluated for hepatoprotective and curative properties against $CCl_4$-induced toxicity, using male albino rats as an experimental model by analyzing aspartate transaminase, alanine transaminase, and alkaline phosphatase in plasma and malondialdehyde content in plasma and liver tissues. Histopathological analysis of liver tissue was also included. Results showed that BT, ET, and KT have the potential to revert the $CCl_4$-induced hepatotoxicity. Among the three types of teas tried, KT was found to be more efficient than BT and ET. Antioxidant molecules produced during the fermentation period could be the reason for the efficient hepatoprotective and curative properties of KT against $CCl_4$-induced hepatotoxicity.
Keywords
Kombucha tea; tea fungus; carbon tetrachloride;
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1 Nevin, K. G. and P. L. Vijayammal. 2005. Effect of Aerva lanata against hepatotoxicity of carbon tetrachloride in rats. Environ. Toxicol. Pharmacol. 20: 471-477   DOI   ScienceOn
2 Yang, Y. S., T. H. Ahn, J. C. Lee, C. J. Moon, and S. H. Kim, 2008. Protective effects of pycnogenol on carbon tetrachlorideinduced hepatotoxicity in Sprague-Dawley rats. Food Chem. Toxicol. 46: 380-387   DOI   ScienceOn
3 Humason, G. L. 1979. Animal Tissue Techniques, 4th Ed. Freeman, San Fransisco, CA
4 Jadon, A., M. Bhadauria, and S. Shukla. 2007. Protective effect of Terminalia belerica Roxb. and gallic acid against carbon tetrachloride induced damage in albino rats. J. Ethnopharmol. 109: 214-218   DOI   ScienceOn
5 Jain, A., M. S. L. Deb, A. Jain, S. P. Rout, V. B. Gupta, and K. L. Krishna. 2008. Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Momordica dioica oxb. leaves. J. Ethnopharmacol. 115: 61-66   DOI   PUBMED   ScienceOn
6 Jeon, T. I., S. G. Hwang, N. G. Park, Y. R. Jung, S. I. Shin, S. D. Choi, and D. K. Park. 2003. Antioxidative effect of chitosan on chronic carbon tetrachloride induced hepatic injury in rats. Toxicology 187: 67-73   DOI   ScienceOn
7 Hesseltine, C. W. 1965. A millenium of fungi, food and fermentation. Mycologia 5: 149-197
8 Jayabalan, R., S. Marimuthu, and K. Swaminathan. 2007. Changes in content of organic acids and tea polyphenols during Kombucha fermentation. Food Chem. 102: 392-398   DOI   ScienceOn
9 Sai Ram, M., B. Anju, T. Pauline, P. Dipti, A. K. Kain, S. S. Mongia, et al. 2000. Effect of Kombucha tea on chromate(VI)- induced oxidative stress in albino rats. J. Ethnopharmacol. 71: 235-240   DOI   PUBMED   ScienceOn
10 Cancalon, P. F. and M. E. Parish. 1995. Changes in the chemical compositions of orange juice during growth of Saccharomyces cerevisiae and Gluconobacter oxydans. Food Microbiol. 12: 117-124   DOI   ScienceOn
11 Lee, H. S., H. H. Kim, and S. K. Ku. 2008. Hepatoprotective effects of Artemisiae capillaris Herba and Picrorrhiza rhizoma combinations on carbon tetrachloride-induced subacute liver damage in rats. Nutr. Res. 28: 270-277   DOI   ScienceOn
12 Fadhel, Z. A. and S. Amran. 2002. Effects of black tea extract on carbon tetrachloride induced lipid peroxidation in liver, kidneys and testes of rats. Phytother. Res. 16: S28-S32
13 Blanc, P. J. 1996. Characterization of tea fungus metabolites. Biotechnol. Lett. 18: 139-142   DOI
14 CPSCEA guidelines for laboratory animal facility. 2003. Indian J. Pharmacol. 35: 257-274
15 Duncan, D. B. 1955. Multiple range "F" tests. Biometrics 11: 1-42   DOI   ScienceOn
16 Pauline, T., P. Dipti, B. Anju, S. Kavimani, S. K. Sharma, A. K. Kain, et al. 2001. Studies on toxicity, anti-stress and hepatoprotective properties of Kombucha tea. Biomed. Environ. Sci. 14: 207-213
17 Reitman, S. and S. A. Frankel, 1957. Colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28: 56   DOI   PUBMED   ScienceOn
18 He, P., Y. Noda, and K. Sugiyama. 2001. Green tea suppresses lipopolysaccharide-induced liver injury in D-galactosaminesensitized rats. J. Nutr. 131: 1560-1567   DOI   ScienceOn
19 Chu, S. C. and C. Chen. 2006. Effect of origins and fermentation time on the antioxidant activities of Kombucha. Food Chem. 98: 502-507   DOI   ScienceOn
20 Kumaravelu, P., D. P. Dakshinamoorthy, S. Subramaniam, H. Devaraj, and N. S. Devaraj. 1995. Effect of eugenol on drugmetabolizing enzymes of carbon tetrachloride-intoxicated rat liver. Biochem. Pharmacol. 49: 1703-1707   DOI   ScienceOn
21 Brent, J. A. and B. H. Rumack. 1993. Role of free radicals in toxic hepatic injury. Clin. Toxicol. 31: 173-196   DOI   ScienceOn
22 Chen, C. and B. Y. Liu. 2000. Changes in major components of tea fungus metabolites during prolonged fermentation. J. Appl. Microbiol. 89: 834-839   DOI   ScienceOn
23 Elhalwagy, M. E. A., N. S. Darwish, and E. M. Zaher. 2008. Prophylactic effect of green tea polyphenols against liver and kidney injury induced by fenitrothion insecticide. Pesticide Biochem. Physiol. 91: 81-89   DOI   ScienceOn
24 Zhen, M. C., Q. Wang, X. H. Huang, L. Q. Cao, X. L. Chen, K. Sun, Y. J. Liu, W. Li, and L. J. Zhang. 2007. Green tea polyphenol epigallocatechin-3-gallate inhibits oxidative damage and preventive effects on carbon tetrachloride-induced hepatic fibrosis. J. Nutr. Biochem. 18: 795-805   DOI   ScienceOn
25 Gong, Y., C. Han, and J. Chen. 2000. Effect of tea polyphenols and tea pigments on the inhibition of precancerous liver lesions in rats. Nutr. Cancer 38: 81-86   DOI   ScienceOn
26 Lin, H. M., H. C. Tseng, C. J. Wang, J. J. Lin, C. W. Lo, and F. P. Chou. 2008. Hepatoprotective effects of Solanum nigrum Linn extract against $CCl_4$-induced oxidative damage in rats. Chem. Biol. Inter. 171: 283-293   DOI   PUBMED   ScienceOn
27 Nichans, W. G. and B. Samuelson. 1968. Formation of MDA from phospholipid arachidonate during microsomal lipid peroxidation. Eur. J. Biochem. 6: 126-130   DOI   ScienceOn
28 Glenn, J. K., M. A. Morgan, M. B. Mayfield, M. Kuwahara, and M. H. Gold. 1983. An extracellular $H_2O_2$ requiring enzyme preparation involved in lignin biodegradation by the white rot basidiomycete. Biochem. Biophys. Res. Commun. 114: 1077- 1083   DOI   ScienceOn
29 King, E. and A. R. Armstong. 1934. Determination of serum and bile phosphastase activity. Can. Med. Assoc. J. 31: 376- 378   PUBMED