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http://dx.doi.org/10.4014/jmb.0904.04054

A High-Yielding, Generic Fed-Batch Process for Recombinant Antibody Production of GS-Engineered Cell Lines  

Fan, Li (The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology)
Zhao, Liang (The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology)
Sun, Yating (The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology)
Kou, Tianci (The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology)
Zhou, Yan (The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology)
Tan, Wen-Song (The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology)
Publication Information
Journal of Microbiology and Biotechnology / v.19, no.12, 2009 , pp. 1695-1702 More about this Journal
Abstract
An animal-component-free and chemically defined fed-batch process for GS-engineered cell lines producing recombinant antibodies has been developed. The fed-batch process relied on supplying sufficient nutrients to match their consumption, simultaneously minimizing the accumulation of by-products (lactate and osmolality). The proportionalities of nutritional consumption were determined by direct analysis. The robust, metabolically responsive feeding strategy was based on the offline measurement of glucose. The fed-batch process was shown to perform equivalently in GS-CHO and GS-NS0 cultures. Compared with batch cultures, the fed-batch technology generated the greater increase in cell yields (5-fold) and final antibody concentrations (4-8-fold). The majority of the increase in final antibody concentration was a function of the increased cell density and the prolonged culture time. This generic and high-yielding fed-batch process would shorten development time, and ensure process stability, thereby facilitating the manufacture of therapeutic antibodies by GS-engineered cell lines.
Keywords
High-yielding; generic fed-batch process; GS-engineered cell lines; glucose; recombinant antibody;
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