Browse > Article

Change in Proteomic Profiles of Genetically Modified 1,3-Propanediol-Producing Recombinant E. coli  

Jin, Li-Hua (Department of Chemical Engineering, Chosun University)
Lee, Jung-Heon (Department of Chemical Engineering, Chosun University)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.8, 2008 , pp. 1439-1444 More about this Journal
Abstract
The recombinant E. coli $\Delta$6 mutant (galR, glpK, gldA, IdhA, lacI, tpiA) was used to produce 1,3-propanediol (PD) from glucose. The 1,3-PD production increased with feedback control of the glucose concentration using fed-batch fermentation. The maximum 1,3-PD concentration produced was 43 g/l after 60 h of fermentation. Glycerol production was minimized when controlling the glucose concentration at less than 1 g/l. The expression levels of seven enzymes related to the 1,3-PD production metabolism were compared during the cell growth phase and 1,3-PD production phase, and their expression levels all increased during 1,3-PD production, with the exception of alcohol dehydrogenase.
Keywords
1,3-Propanediol; glycerol; proteomics; fed-batch fermentation;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
연도 인용수 순위
1 Boenigk, R., S. Bowien, and G. Gottschalk. 1993. Fermentation of glycerol to 1,3-propanediol in continuous cultures of Citrobacter freundii. Appl. Microbiol. Biotechnol. 38: 453-457
2 Gonzalez-Pajuelo, M., I. Meynial-Salles, F. Mendes, J. C. Andrade, I. Vasconcelos, and P. Soucaille. 2005. Metabolic engineering of Clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol. Metab. Eng. 7: 329-336   DOI   ScienceOn
3 Knietsch, A., S. Bowien, G. Whited, G. Gottschalk, and R. Daniel. 2003. Identification and characterization of coenzyme B12-dependent glycerol dehydratase- and diol dehydratase-encoding genes from metagenomic DNA libraries derived from enrichment cultures. Appl. Environ. Microbiol. 69: 3048-3060   DOI   ScienceOn
4 Papanikolaou, S., P. Ruiz-Sanchez, B. Pariset, F. Blanchard, and M. Fick. 2000. High production of 1,3-propanediol from industrial glycerol by a newly isolated Clostridium butyricum strain. J. Biotechnol. 77: 191-208   DOI
5 Wang, W., J. Sun, M. Nimtz, W.-D. Deckwer, and A.-P. Zeng. 2003. Protein identification from two-dimensional gel electrophoresis analysis of Klebsiella pneumoniae by combined use of mass spectrometry data and raw genome sequences. Proteome Sci. 1: 1-9   DOI   ScienceOn
6 Wilke, D. 1999. Chemicals from biotechnology: Molecular plant genetics will challenge the chemical and the fermentation industry. Appl. Microbiol. Biotechnol. 52: 135-145   DOI   ScienceOn
7 Nakamura, C. E. and G. M. Whited. 2003. Metabolic engineering for the microbial production of 1,3-propanediol. Curr. Opin. Biotechnol. 14: 454-459   DOI   ScienceOn
8 Carole, T. M., J. Pellegrino, and M. D. Paster. 2004. Opportunities in the industrial biobased products industry. Appl. Biochem. Biotechnol. 113-116: 871-885
9 Lee, S. Y., S. H. Hong, S. H. Lee, and S. J. Park. 2004. Fermentative production of chemicals that can be used for polymer synthesis. Macromol. Biosci. 4: 157-164   DOI   ScienceOn
10 Lee, S. Y. and D. Jeoung. 2007. The reverse proteomics for identification of tumor antigens. J. Microbiol. Biotechnol. 17: 879-890   과학기술학회마을
11 Skraly, F. A., B. L. Lytle, and D. C. Cameron. 1998. Construction and characterization of a 1,3-propanediol operon. Appl. Environ. Microbiol. 64: 98-105
12 Tong, I. T., H. H. Liao, and D. C. Cameron. 1991. 1,3-Propanediol production by Escherichia coli expressing genes from the Klebsiella pneumoniae dha regulon. Appl. Environ. Microbiol. 57: 3541-3546
13 Altaras, N. E. and D. C. Cameron. 1999. Metabolic engineering of a 1,2-propanediol pathway in Escherichia coli. Appl. Environ. Microbiol. 65: 1180-1185
14 Abbad-Andaloussi, S., C. Manginot-Durr, J. Amine, E. Petitdemange, and H. Petitdemange. 1995. Isolation and characterization of Clostridium butyricum DSM 5431 mutants with increased resistance to 1,3-propanediol and altered production of acids. Appl. Environ. Microbiol. 61: 4413-4417
15 Huang, H., C. S. Gong, and G. T. Tsao. 2002. Production of 1,3-propanediol by Klebsiella pneumoniae. Appl. Biochem. Biotechnol. 98-100: 687-698   DOI   ScienceOn
16 Barbirato, F., E. H. Himmi, T. Conte, and A. Bories. 1998. 1,3-Propanediol production by fermentation: An interesting way to valorize glycerin from the ester and ethanol industries. Industr. Crops Prod. 7: 281-289   DOI   ScienceOn
17 Xiu, Z.-L., B.-H. Song, Z.-T. Wang, L.-H. Sun, E.-M. Feng, and A.-P. Zeng. 2004. Optimization of dissimilation of glycerol to 1,3-propanediol by Klebsiella pneumoniae in one- and two-stage anaerobic cultures. Biochem. Eng. J. 19: 189-197   DOI   ScienceOn
18 Hartlep, M., W. Hussmann, N. Prayitno, I. Meynial-Salles, and A. P. Zeng. 2002. Study of two-stage processes for the microbial production of 1,3-propanediol from glucose. Appl. Microbiol. Biotechnol. 60: 60-66   DOI   ScienceOn
19 Colin, T., A. Bories, and G. Moulin. 2000. Inhibition of Clostridium butyricum by 1,3-propanediol and diols during glycerol fermentation. Appl. Microbiol. Biotechnol. 54: 201-205   DOI   ScienceOn
20 Kim, I., H. Yun, and I. Jin. 2007. Comparative proteomic analyses of the yeast Saccharomyces cerevisiae KNU5377 strain against menadione-induced oxidative stress. J. Microbiol. Biotechnol. 17: 207-217   과학기술학회마을
21 Chotani, G., T. Dodge, A. Hsu, M. Kumar, R. LaDuca, D. Trimbur, W. Weyler, and K. Sanford. 2000. The commercial production of chemicals using pathway engineering. Biochim. Biophys. Acta 1543: 434-455   DOI   ScienceOn
22 Menzel, K., A. P. Zeng, and W. D. Deckwer. 1997. High concentration and productivity of 1,3-propanediol from continuous fermentation of glycerol by Klebsiella pneumoniae. Enzyme Microbial Technol. 20: 82-86   DOI   ScienceOn
23 Seul, K. J., S. H. Park, C. M. Ryu, Y. H. Lee, and S. Y. Ghim. 2007. Proteome analysis of Paenibacillus polymyxa E681 affected by barley. J. Microbiol. Biotechnol. 17: 934-944   과학기술학회마을
24 Biebl, H., K. Menzel, A. P. Zeng, and W. D. Deckwer. 1999. Microbial production of 1,3-propanediol. Appl. Microbiol. Biotechnol. 52: 289-297   DOI   ScienceOn
25 Cameron, D. C., N. E. Altaras, M. L. Hoffman, and A. J. Shaw. 1998. Metabolic engineering of propanediol pathways. Biotechnol. Prog. 14: 116-125   DOI   ScienceOn
26 Zhu, M. M., P. D. Lawman, and D. C. Cameron. 2002. Improving 1,3-propanediol production from glycerol in a metabolically engineered Escherichia coli by reducing accumulation of sn-glycerol-3-phosphate. Biotechnol. Prog. 18: 694-699   DOI   ScienceOn
27 Lee, Y. J. and K. H. Jung. 2007. Modulation of the tendency towards inclusion body formation of recombinant protein by the addition of glucose in the araBAD promoter system of Escherichia coli. J. Microbiol. Biotechnol. 17: 1898-1903   과학기술학회마을