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Isolation of Sorangium cellulosum Carrying Epothilone Gene Clusters  

Hyun, Hye-Sook (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Chung, Jin-Woo (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Kim, Ji-Hoon (Molecular Cancer Research Center, Korea Research Institute of Bioscience and Biotechnolgoy (KRIBB))
Lee, Jong-Suk (Molecular Cancer Research Center, Korea Research Institute of Bioscience and Biotechnolgoy (KRIBB))
Kwon, Byoung-Mog (Molecular Cancer Research Center, Korea Research Institute of Bioscience and Biotechnolgoy (KRIBB))
Son, Kwang-Hee (Molecular Cancer Research Center, Korea Research Institute of Bioscience and Biotechnolgoy (KRIBB))
Cho, Kyung-Yun (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.8, 2008 , pp. 1416-1422 More about this Journal
Abstract
Epothilone and its analogs are a potent new class of anticancer compounds produced by myxobacteria. Thus, in an effort to identify new myxobacterial strains producing epothilone and its analogs, cellulose-degrading myxobacteria were isolated from Korean soils, and 13 strains carrying epothilone biosynthetic gene homologs were screened using a polymerase chain reaction. A migration assay revealed that Sorangium cellulosum KYC3013, 3016, 3017, and 3018 all produced microtubule-stabilizing compounds, and an LC-MS/MS analysis showed that S. cellulosum KYC3013 synthesized epothilone A.
Keywords
Myxobacteria; epothilone; Sorangium cellulosum;
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