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Asp97 is a Crucial Residue Involved in the Ligation of the [$Fe_4S_4$] Cluster of IscA from Acidithiobacillus ferrooxidans  

Jiang, Huidan (Department of Bioengineering, School of Resources Processing and Bioengineering, Central South University)
Zhang, Xiaojian (Department of Bioengineering, School of Resources Processing and Bioengineering, Central South University)
Ai, Chenbing (Department of Bioengineering, School of Resources Processing and Bioengineering, Central South University)
Liu, Yuandong (Department of Bioengineering, School of Resources Processing and Bioengineering, Central South University)
Liu, Jianshe (Department of Bioengineering, School of Resources Processing and Bioengineering, Central South University)
Qiu, Guanahou (Department of Bioengineering, School of Resources Processing and Bioengineering, Central South University)
Zeng, Jia (Department of Bioengineering, School of Resources Processing and Bioengineering, Central South University)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.6, 2008 , pp. 1070-1075 More about this Journal
Abstract
IscA was proposed to be involved in the iron-sulfur cluster assembly encoded by the iscSUA operon, but the role of IscA in the iron-sulfur cluster assembly still remains controversial. In our previous study, the IscA from A. ferrooxidans was successfully expressed in Escherichia coli, and purified to be a [$Fe_4S_4$] -cluster-containing protein. Cys35, Cys99, and Cys101 were important residues in ligating with the [$Fe_4S_4$] cluster. In this study, Asp97 was found to be another ligand for the iron-sulfur cluster binding according to site-directed mutagenesis results. Molecular modeling for the IscA also showed that Asp97 was a strong ligand with the [$Fe_4S_4$] cluster, which was in good agreement with the experimental results. Thus, the [$Fe_4S_4$] cluster in IscA from A. ferrooxidans was ligated by three cysteine residues and one aspartic acid.
Keywords
IscA; Acidithiobacillus ferrooxidans; mutation; iron-sulfur cluster; molecular modeling;
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