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Diversity of the Lichenized Fungi in King George Island, Antarctica, Revealed by Phylogenetic Analysis of Partial Large Subunit rDNA Sequences  

Lee, Jin-Sung (Polar BioCenter, Korea Polar Research Institute, KORDI)
Lee, Hong-Kum (Polar BioCenter, Korea Polar Research Institute, KORDI)
Hur, Jae-Seoun (Department of Environmental Education, Sunchon National University)
Andreev, Mikhail (Komarov Botanical Institute)
Hong, Soon-Gyu (Polar BioCenter, Korea Polar Research Institute, KORDI)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.6, 2008 , pp. 1016-1023 More about this Journal
Abstract
Lichens are predominant and important components of flora in the terrestrial ecosystem of Antarctica. However, relatively few researches on the phylogenetic position of Antarctic lichen-forming fungi have been accomplished. In this study, partial sequences of nuclear large subunit rDNAs from 50 Antarctic specimens were obtained and the phylogeny was reconstructed. Antarctic lichen species were distributed in 4 orders, including the monophyletic order Agyrales, paraphyletic orders Pertusariales and Teloschistales, and polyphyletic order Lecanorales. Species diversity was highest in the order Lecanorales, followed by Teloschistales and Pertusariales. Based on the phylogeny and sequence similarity analyses, it is proposed that the taxonomy of Stereocaulon alpinum, Physcia caesia, Usnea aurantiacoatra, and Cladonia species should be revised by careful examination of their phenotypic and molecular characteristics. Six species known to be endemic to Antarctica, Catillaria corymbosa, Himantormia lugubris, Leptogium puberulum, Pertusaria pertusa, Rhizoplaca aspidophora, and Umbilicaria antarctica, formed unique lineages, implying independent origins in the Antarctic area.
Keywords
Antarctica; diversity; King George Island; lichens; phylogeny;
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1 Bhattacharya, D., F. Lutzoni, V. Reeb, D. Simon, J. Nason, J. and F. Fernandez. 2000. Widespread occurrence of spliceosomal introns in the rDNA genes of Ascomycetes. Mol. Biol. Evol. 17: 1971-1984   DOI   ScienceOn
2 Hawksworth, D. H. 2006. Pandora's mycological box: Molecular sequences vs. morphology in understanding fungal relationships and biodiversity. Rev. Iberoam. Micol. 23: 127-133   DOI   ScienceOn
3 James, T. Y., F. Kauff, C. L. Schoch, P. B. Matheny, V. Hofstetter, C. Y. Cox, et al. 2006. Reconstructing the early evolution of Fungi using a six-gene phylogeny. Nature 443: 818-822   DOI   ScienceOn
4 Jeon, Y. S., H. Chung, S. Park, I. Hur, J. H. Lee, and J. Chun. 2005. jPHYDIT: A JAVA-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21: 3171-3173   DOI   ScienceOn
5 Lutzoni, F., F. Kauff, C. J. Cox, D. McLaughlin, G. Celio, B. Dentinger, et al. 2004. Assembling the fungal tree of life: Progress, classification, and evolution of subcellular traits. Am. J. Bot. 91: 1446-1480   DOI   ScienceOn
6 Redón, J. F. 1985. Liquenes Antarticos. Instituto Antártico Chileno, Santiago
7 Rehner, S. A. and G. J. Samuels. 1994. Taxonomy and phylogeny of Gliocladium analyzed from nuclear large subunit ribosomal DNA sequences. Mycol. Res. 98: 625-634   DOI   ScienceOn
8 Seymour, F. A., P. D. Crittenden, N. Wirtz, D. O. Ovstedal, P. S. Dyer, and H. T. Lumbsch. 2007. Phylogenetic and morphological analysis of Antarctic lichen-forming Usnea species in the group Neuropogon. Antarctic Sci. 19: 71-82
9 Wirtz, N., C. Printzen, L. G. Sancho, and T. H. Lumbsch. 2006. The phylogeny and classification of Neuropogon and Usnea (Parmeliaceae, Ascomycota) revisited. Taxon 55: 367-376   DOI   ScienceOn
10 Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111-120   DOI
11 Miadlikowska, J., F. Kauff, V. Hofsteller, E. Fraker, M. Grube, J. Hafellner, et al. 2006. New insights into classification and evolution of the Lecanoromycetes (Pezizomycotina, Ascomycota) from phylogenetic analyses of three ribosomal RNA- and two protein-coding genes. Mycologia 98: 1088-1103   DOI   ScienceOn
12 Osyczka, P. and M. Olech. 2005. The lichen genus Cladonia of King George Island, South Shetland Islands, Antarctica. Polish Polar Res. 26: 107-123
13 Gardes, M. and T. D. Bruns. 1993. ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Mol. Ecol. 2: 113-118   DOI   ScienceOn
14 Olech, M. 2004. Lichens of King George Island, Antarctica. The Institute of Botany of the Jagiellonian University, Krakow, Poland
15 Ott, S., M. Brinkmann, N. Wirtz, and H. T. Lumbsch. 2004. Mitochondrial and nuclear ribosomal DNA data do not support separation of the Antarctic lichens Umbilicaria kappenii and Umbilicaria antarctica as distinct species. Lichenologist 36: 227-234   DOI
16 Kroken, S. and J. W. Taylor. 2001. A gene genealogical approach to recognize phylogenetic species boundaries in the lichenized fungus Letharia. Mycologia 93: 38-53   DOI   ScienceOn
17 Hognabba, F. 2006. Molecular phylogeny of the genus Stereocaulon (Stereocaulaceae, lichenized ascomycetes). Mycol. Res. 110: 1080-1092   DOI   ScienceOn
18 Huelsenbeck, J. P. and F. Ronquist. 2001. MrBayes: Bayesian inference of phylogeny. Bioinformatics 17: 754-755   DOI   ScienceOn
19 Inoue, M. 1993. Floristic notes on lichens in the Fildes Peninsula of King George Island and Harmony Cove of Nelson Island, South Shetland Islands, the Antarctic. Proceedings of the NIPR Symposium on Polar Biology 6: 106-120
20 Posada, D. and K. A. Crandall. 1988. MODELTEST: Testing the model of DNA substitution. Bioinformatics 14: 817-818   DOI   ScienceOn
21 Printzen, C. 2002. Fungal specific primers for PCR-amplification of mitochondrial LSU in lichens. Mol. Ecol. Notes 2: 130-132   DOI   ScienceOn
22 Swofford, D. L. 2002. PAUP*4.0b Phylogenetic Analysis Using Parsimony (* and other method), version 4. Sinauer Associates, Sunderland, Massachusetts, U.S.A
23 Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin, and D. G. Higgins. 1997. The ClustalX Windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acids Res. 24: 4876-4882
24 Myllys, L., S. Stenroos, A. Thell, and T. Ahti. 2003. Phylogeny of bipolar Cladonia arbuscula and Cladonia mitis (Lecanorales, Euascomycetes). Mol. Phyl. Evol. 27: 58-69   DOI   ScienceOn
25 Kim, J. H., I.-Y. Ahn, K. S. Lee, H. Chung, and H. G. Choi. 2007. Vegetation of Barton Peninsula in the neighborhood of King Sejong Station (King George Island, maritime Antarctic). Polar Biol. 30: 903-916   DOI   ScienceOn
26 Vilgalys, R. and M. Hester. 1990. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J. Bacteriol. 172: 4238-4246   DOI
27 Articus, K. 2004. Neuropogon and the phylogeny of Usnea s.l. (Parmeliaceae, Lichenized Ascomycetes). Taxon 53: 925- 934   DOI   ScienceOn
28 Kim, J. H., I.-Y. Ahn, S. G. Hong, M. Andreev, K.-M. Lim, M. J. Oh, Y. J. Koh, and J.-S. Hur. 2006. Lichen flora around the Korean Antarctic Scientific Station, King George Island, Antarctic. J. Microbiol. 44: 480-491   과학기술학회마을
29 Stenroos, S., J. Hyvönen, L. Myllys, A. Thell, and T. Ahti. 2002. Phylogeny of the genus Cladonia s.lat. (Cladoniaceae, Ascomycetes) inferred from molecular, morphological, and chemical data. Cladistics 18: 237-278   DOI   ScienceOn
30 White, T. J., T. D. Bruns, S. B. Lee, and J. W. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315-322. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White (eds.), PCR Protocols: A Guide to Methods and Application. Academic Press, San Diego, U.S.A
31 Andreev, M. P. 1988. De lichen flora Paeninsulae Fildes Insulae King George (Antarctic) notula. Novosti Sistematiki Nizshikh Rastenii 25: 118-124
32 Olech, M. 2001. Annotated Checklist of Antarctic Lichens and Lichenicolous Fungi. The Institute of Botany of the Jagiellonian University, Kraków, Poland
33 $\phi$vstedal, D. O. and R. I. Lewis Smith. 2001. Lichens of Antarctica and South Georgia: A Guide to their Identification and Ecology. Cambridge University Press, Cambridge, U.K
34 Dodge, C. W. 1973. Lichen Flora of the Antarctic Continent and Adjacent Islands. Phoenix Publishing, Canaan, New Hampshire
35 Blackwell, M., D. S. Hibbett, J. W. Taylor, and J. W. Spatafora. 2006. Research coordination networks: A phylogeny for kingdom Fungi (Deep Hypha). Mycologia 98: 829-837   DOI   ScienceOn
36 Hibbett, D. S., M. Binder, J. F. Bischoff, M. Blackwell, P. F. Cannon, O. Eriksson, et al. 2007. A higher-level phylogenetic classification of the Fungi. Mycol. Res. 111: 509-547   DOI   ScienceOn
37 Simon, D. M., C. L. Hummel, S. L. Sheeley, and D. Bhattacharya. 2005. Heterogeneity of intron presence or absence in rDNA genes of the lichen species Physcia aipolia and P. stellaris. Curr. Genet. 47: 389-399   DOI   ScienceOn
38 Eriksson, O. E. 2006. Outline of Ascomycota-2006. Myconet 12: 1-82
39 DePriest, P. T. 1993. Small subunit rDNA variation in a population of lichen fungi due to optional group-I introns. Gene 134: 67-74   DOI   ScienceOn
40 Thomson, J. W. 1984. American Arctic Lichens. 1. The Macrolichens. Columbia University Press, New York
41 Summerfield, T. C. and J. J. Eaton-Rye. 2006. Pseudocyphellaria crocata, P. neglecta and P. perpetua from the northern and southern hemispheres are a phylogenetic species and share cyanobionts. New Phytologist 170: 597-607   DOI   ScienceOn
42 Reeb, V., F. Lutzoni, and C. Roux. 2004. Contribution of RPB2 to multilocus phylogenetic studies of the Pezizomycotina (euascomycetes, Fungi) with special emphasis on the lichenforming Acarosporaceae and evolution of polyspory. Mol. Phyl. Evol. 32: 1036-1060   DOI   ScienceOn