Effects of -Mercaptoethanol and Hydrogen Peroxide on Enzymatic Conversion of Human Proinsulin to Insulin |
Son, Young-Jin
(Interdisciplinary Program for Bioengineering, Seoul National University)
Kim, Chang-Kyu (CKDBiO Research Institute, CKDBiO) Choi, Byoung-Taek (CKDBiO Research Institute, CKDBiO) Park, Yong-Cheol (Center for Agricultural Biomaterials, Seoul National University) Seo, Jin-Ho (Interdisciplinary Program for Bioengineering, Seoul National University) |
1 | Chen, J. Q., H. T. Zhang, M. H. Hu, and J. G. Tang. 1995. Production of human insulin in an Escherichia coli system with Met-Lys-human proinsulin as the expressed precursor. Appl. Biochem. Biotechnol. 55: 5-15 DOI ScienceOn |
2 | Kemmler, W., J. D. Peterson, and D. F. Steiner. 1971. Studies on conversion of proinsulin to insulin. 1. Conversion in vitro with trypsin and carboxypeptidase B. J. Biol. Chem. 246: 6786-6791 |
3 | Klyushnichenko, V. E., D. M. Koulich, S. A. Yakimov, K. V. Maltsev, G. A. Grishina, I. V. Nazimov, and A. N. Wulfson. 1994. Recombinant human insulin: III. High-performance liquid chromatography and high-performance capillary electrophoresis control in the analysis of step-by-step production of recombinant human insulin. J. Chromatogr. A 661: 83-92 DOI |
4 | Mergulhao, F. J. M. and G. A. Monteiro. 2007. Analysis of factors affecting the periplasmic productiion of recombinant proteins in Escherichia coli. J. Microbiol. Biotechnol. 17: 1236-1241 과학기술학회마을 |
5 | Nielsen, R. G., G. S. Sittampalam, and E. C. Rickard. 1989. Capillary zone electrophoresis of insulin and growth-hormone. Anal. Biochem. 177: 20-26 DOI ScienceOn |
6 | Winter, J., H. Lilie, and R. Rudolph. 2002. Renaturation of human proinsulin - a study on refolding and conversion to insulin. Anal. Biochem. 310: 148-155 DOI ScienceOn |
7 | Phue, J. N., S. J. Oh, Y. J. Son, Y. I. Kim, K. H. Kim, J. W. Kim, C. I. Hong, I. S. Chung, and T. R. Hahn. 2000. Improved refolding of recombinant human proinsulin from Escherichia coli in a two-stage reactor system. J. Microbiol. Biotechnol. 10: 75-80 |
8 | Son, Y. J., K. H. Park, S. Y. Lee, S. J. Oh, C. K. Kim, B. T. Choi, Y. C. Park, and J. H. Seo. 2007. Effects of temperature shift strategies on production of human preproinsulin in fed-batch fermentation of recombinant Escherichia coli. Biotechnol. Bioprocess Eng. [In Press] 과학기술학회마을 DOI |
9 | Castellanos-Serra, L. R., E. Hardy, R. Ubieta, N. S. Vispo, C. Fernandez, V. Besada, V. Falcon, M. Gonzalez, A. Santos, G. Perez, A. Silva, and L. Herrera. 1996. Expression and folding of an interleukin-2-proinsulin fusion protein and its conversion into insulin by a single step enzymatic removal of the C-peptide and the N-terminal fused sequence. FEBS Lett. 378: 171-176 DOI ScienceOn |
10 | Jonasson, P., J. Nilsson, E. Samuelsson, T. Moks, S. Stahl, and M. Uhlen. 1996. Single-step trypsin cleavage of a fusion protein to obtain human insulin and its C peptide. Eur. J. Biochem. 236: 656-661 DOI ScienceOn |
11 | Yon, J. M. and J. M. Betton. 1991. Protein folding in vitro and in the cellular environment. Biol. Cell 71: 17-23 DOI |
12 | Middelberg, A. P. J. 2002. Preparative protein refolding. Trends Biotechnol. 20: 437-443 DOI ScienceOn |
13 | Ladisch, M. R. and K. L. Kohlmann. 1992. Recombinant human insulin. Biotechnol. Prog. 8: 469-478 DOI ScienceOn |
14 | Kim, S. G., J. A. Kim, H. A. Yu, D. H. Lee, D. H. Kweon, and J. H. Seo. 2006. Application of poly-arginine fused minichaperone to renaturation of cyclodextrin glycosyltransferase expressed in recombinant Escherichia coli. Enzyme Microb. Technol. 39: 459-465 DOI ScienceOn |
15 | Song, J. H., J. S. Won, Y. C. Lee, and M. H. Choe. 2006. Increase refolding yield of disulfide bond bridged fab-toxin homodimers by the insertion of CH3 domains. J. Microbiol. Biotechnol. 16: 1104-1110 과학기술학회마을 |
16 | Fischer, B., I. Sumner, and P. Goodenough. 1993. Isolation, renaturation, and formation of disulfide bonds of eukaryotic proteins expressed in Escherichia coli as inclusion bodies. Biotechnol. Bioeng. 41: 3-13 DOI |
17 | Lukacsovich, T., G. Baliko, A. Orosz, E. Balla, and P. Venetianer. 1990. New approaches to increase the expression and stability of cloned foreign genes in Escherichia coli. J. Biotechnol. 13: 243-250 DOI ScienceOn |
18 | Koo, T. Y. and T. H. Park. 2007. Expression of recombinant human growth hormone in a soluble form in Escherichia coli by slowing down the protein synthesis rate. J. Microbiol. Biotechnol. 17: 579-585 과학기술학회마을 |
19 | Lim, H. K., S. U. Lee, S. I. Chung, K. H. Jung, and J. H. Seo. 2004. Induction of the T7 promoter using lactose for production of recombinant plasminogen kringle 1-3 in Escherichia coli. J. Microbiol. Biotechnol. 14: 225-230 |
20 | Linde, S., B. S. Welinder, and J. H. Nielsen. 1993. Analysis of proinsulin and its conversion products by reversed-phase highperformance liquid chromatography. J. Chromatogr. B 614: 185-204 DOI |