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Isolation of Uncultivable Anaerobic Thermophiles of the Family Clostridiaceae Requiring Growth-Supporting Factors  

Kim, Joong-Jae (Department of Biological Sciences, Graduate School of Science, Osaka University)
Kim, Hee-Na (Department of Biological Sciences, Graduate School of Science, Osaka University)
Masui, Ryoji (Department of Biological Sciences, Graduate School of Science, Osaka University)
Kuramitsu, Seiki (Department of Biological Sciences, Graduate School of Science, Osaka University)
Seo, Jin-Ho (Department of Agricultural Biotechnology, Seoul National University)
Kim, Kwang (Department of Biological Sciences, Graduate School of Science, Osaka University)
Sung, Moon-Hee (Department of Bio & Nanochemistry, Kookmin University, BioLeaders Corporation)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.4, 2008 , pp. 611-615 More about this Journal
Abstract
Novel groups of uncultivable anaerobic thermophiles were isolated from compost by enrichment cultivation in medium with a cell-free extract of Geobacillus toebii. The cell-free extract of G. toebii provided the medium with growth-supporting factors (GSF) needed to cultivate the previously uncultured microorganisms. Twenty-nine GSF-requiring candidates were successfully cultivated, and 16 isolated novel bacterial strains were classified into three different groups of uncultivable bacteria. The similarity among these 16 isolates and a phylogenetic analysis using 16S rRNA gene sequences revealed that these GSF-requiring strains represented novel groups within the family Clostridiaceae.
Keywords
Growth-supporting factor (GSF); uncultivable bacteria; cell-free extract; Geobacillus toebii; commensal thermophile; compost;
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Times Cited By KSCI : 6  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
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