Browse > Article

Genetic and Physiological Characterization of Oxytetracycline-Resistant Bacteria from Giant Prawn Farms  

Heepngoen, Pimpak (Department of Soil Science, Kasetsart University)
Sajjaphan, Kannika (Department of Soil Science, Kasetsart University)
Ferguson, John A. (Department of Soil, Water, & Climate, and Bio Technology Institute, University of Minnesota)
Sadowsky, Michael J. (Department of Soil, Water, & Climate, and Bio Technology Institute, University of Minnesota)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.2, 2008 , pp. 199-206 More about this Journal
Abstract
Four hundred and thirteen oxytetracycline-resistant bacteria were recovered from six freshwater giant prawn farms with a history of oxytetracycline use. Most oxytetracycline-resistant isolates were Gram-negative bacteria. Six groups of oxytetracycline-resistant bacteria were classified using cluster analysis based on a comparison of levels of oxytetracycline resistance. Complex fingerprint patterns were obtained for 71 isolates studied. In general, the band patterns of isolates from different ponds were very similar, and the data indicated that the isolates were closely related. The exploration for cross-resistance found that most of the 71 oxytetracycline-resistant isolates were also resistant to tetracycline and chlortetracycline, but had a relatively low resistance to doxycycline. Many isolates showed higher chlortetracycline resistance than oxytetracycline resistance. Additionally, the oxytetracycline-resistant isolates were examined for the presence of tetracycline resistance (tet) genes. Fifty percent of the isolates carried one of the 14 known tet genes examined. The most common determinants were TetA and TetD. However, TetB, TetC, TetE, TetK, TetL, and TetM were also found with various frequencies.
Keywords
Oxytetracycline-resistant bacteria; freshwater giant prawn; antibiotics;
Citations & Related Records

Times Cited By Web Of Science : 1  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Bryan, A., N. Shapir, and M. J. Sadowsky. 2004. Frequency and distribution of tetracycline resistance genes in genetically diverse, nonselected, and nonclinical Escherichia coli strains isolated from diverse human and animal sources. Appl. Environ. Microbiol. 70: 2503-2507   DOI   ScienceOn
2 Coyne, R., M. Hiney, and P. Smith. 1997. Transient presence of oxytetracycline in blue mussels (Mytilus edulis) following its therapeutic use at a marine Atlantic salmon farm. Aquaculture 149: 175-181   DOI   ScienceOn
3 Guardabassi, L., A. Dalsgaard, M. Raffatellu, and J. E. Olsen. 2000. Increase in the prevalence of oxolinic acid resistant Acinetobacter spp. observed in a stream receiving the effluent from a freshwater trout farm following the treatment with oxolinic acid-medicated feed. Aquaculture. 188: 205-218   DOI
4 Hawkins, L., H. Hariharan, K. Whitman, G. Johnson, and J. Bryenton. 1997. Drug resistance of a typical Aeromonas salmonicida from Atlantic salmon and rainbow trout in Newfoundland. Bull. Aquacult. Assoc. Can. 2: 39-41
5 Hektoen, H., J. A. Berge, V. Hormazabal, and M. Yndestad. 1995. Persistence of antibacterial agents in marine sediments. Aquaculture 133: 175-184   DOI
6 Ng, L. K., I. Martin, M. Alfa, and M. Mulvey. 2001. Multiplex PCR for the detection of tetracycline resistant genes. Mol. Cell. Probes 15: 209-215   DOI   ScienceOn
7 Nygaard, K., B. T. Lunestad, H. Hektoen, J. A. Berge, and V. Hormazabal. 1992. Resistance to oxytetracycline, oxolinic acid and furazolidone in bacteria from marine sediments. Aquaculture 104: 31-36   DOI   ScienceOn
8 Jacobsen, P. and L. Berglind. 1988. Persistence of oxytetracycline in sediments from fish farms. Aquaculture 70: 365-370   DOI   ScienceOn
9 Barnes, A. C., T. S. Hastings, and S. G. B. Amyes, 1994. Amoxycillin resistance in Scottish isolates of Aeromonas salmonicida. J. Fish Dis. 17: 357-363   DOI   ScienceOn
10 Capone, D. G., D. P. Weston, V. Miller, and C. Shoemaker. 1996. Antibacterial residues in marine sediments and invertebrates following chemotherapy in aquaculture. Aquaculture 145: 55-75   DOI
11 Alderman, D. J., H. Rosenthal, P. Smith, J. Stewart, and D. Weston. 1994. Chemicals used in mariculture, ICES Cooperative Research Report No. 202. ICES, Copenhagen.
12 Johnson, L. K., M. B. Brown, E. A. Carruthers, J. A. Ferguson, P. E. Dombek, and M. J. Sadowsky. 2004. Sample size, library composition, and genotypic diversity among natural populations of Escherichia coli from different animals influence accuracy of determining sources of fecal pollution. Appl. Environ. Microbiol. 70: 4478-4485   DOI   ScienceOn
13 Bangkok Post. 2002. 2001 Year-End Economic Review. Available from
14 Samuelsen, O. B., B. T. Lunestad, B. Husevåg, T. Holleland, and A. Ervik. 1992. Residues of oxolinic acid in wild fauna following medication in fish farms. Dis. Aquat. Org. 12: 111-119   DOI
15 DePaola, A., J. T. Peeler, and G. E. Rodrick. 1995. Effect of oxytetracycline medicated feed on antibiotic resistance of Gramnegative bacteria in catfish ponds. Appl. Environ. Microbiol. 61: 2335-2340
16 $Husev\aa\,g$, B., B. T. Lunestad, P. J. Johannessen, $\phi$. Enger, and O. B. Samuelsen. 1991. Simultaneous occurrence of Vibrio salmonicida and antibiotic resistant bacteria in sediments at abandoned aquaculture sites. J. Fish Dis. 14: 631-640   DOI
17 Kerry, J., R. Coyne, D. Gilroy, M. Hiney, and P. Smith. 1996. Spatial distribution of oxytetracycline and elevated frequencies of oxytetracycline resistance in sediments beneath a marine salmon farm following oxytetracycline therapy. Aquaculture 145: 31-39   DOI
18 $S\phi rum$, H., M. C. Roberts, and J. H. Crosa. 1992. Identification and cloning of a tetracycline resistance gene from the fish pathogen Vibrio salmonicida. Antimicrob. Agents Chemother. 36: 611-615   DOI   ScienceOn
19 Schnick, R. A. 1991. Chemicals for worldwide aquaculture, pp. 441-466. In: Fish Health Management in Asia-Pacific. Report on a Regional Study and Workshop on Fish Disease and Fish Health Management. Asian Development Bank and Network of Aquaculture Centres in Asia, Bangkok, Thailand
20 Woo, S. S., J. Jiang, B. S. Gill, A. H. Paterson, and R. A. Wing. 1994. Construction and characterization of a bacterial artificial chromosome library of Sorghum bicolor. Nucl. Acids Res. 22: 4922-4931   DOI   ScienceOn
21 Lutzhoft, H.-C., B. Halling-Sorensen, and S. E. $Halling-S\phi rensen$. 1999. Algal toxicity of antibacterial agents applied in Danish fish farming. Arch. Environ. Contam. Toxicol. 36: 1-6   DOI   ScienceOn
22 Weston, D. P. 1996. Environmental considerations in the use of antibacterial drugs in aquaculture. In: D. J. Baird (ed.). Aquaculture and Water Resource Management. Blackwell Science, Oxford, U.K.
23 Tonguthai, K. 2000. The use of chemicals in aquaculture in Thailand, pp. 207-220. In: Use of Chemicals in Aquaculture in Asia. Proceedings of the Meeting on the Use of Chemicals in Aquaculture in Asia, Tigbauan, Iloilo, Philippines, 20-22 May 1996
24 Bangkok Post. 2001. 2000 Year-End Economic Review. Available from
25 Bangkok Post. 2001. Shrimp raisers want state help, March 15
26 FAO. 2001. FAO Yearbook. Fishery Statistics. Aquaculture Production, Vol. 88/2. FAO, Rome
27 Miranda, C. D., C. Kehrenberg, C. Ulep, S. Schwarz, and M. C. Roberts. 2003. Diversity of tetracycline resistance genes in bacteria from Chilean salmon farms. Antimicrob. Agents Chemother. 47: 883-888   DOI   ScienceOn