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http://dx.doi.org/10.4014/jmb.0800.210

Proteome Analysis for 3T3-L1 Adipocyte Differentiation  

Rahman, Atiar (Department of Biotechnology, Daegu University)
Kumar, Suresh G. (Department of Biotechnology, Daegu University)
Lee, Sung-Hak (Department of Biotechnology, Daegu University)
Hyun, Sun-Hwang (Department of Biotechnology, Daegu University)
Kim, Hyun-Ah (Department of Biotechnology, Daegu University)
Yun, Jong-Won (Department of Biotechnology, Daegu University)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.12, 2008 , pp. 1895-1902 More about this Journal
Abstract
Adipose tissue is an important endocrine organ involved in the control of whole body energy homeostasis and insulin sensitivity. Considering the increased incidence of obesity and obesity-related disorders, including diabetes, it is important to understand thoroughly the process of adipocyte differentiation and its control. Therefore, we performed a differential proteome mapping strategy using two-dimensional gel electrophoresis combined with peptide mass fingerprinting to identify intracellular proteins that are differentially expressed during adipose conversion of 3T3-L1 pre-adipocytes in response to an adipogenic cocktail. In the current study, we identified 46 differentially expressed proteins, 6 of which have not been addressed previously in 3T3-L1 cell differentiation. Notably, we found that phosphoribosyl pyrophosphate synthetase (PRPS), a regulator of cell proliferation, was preferentially expressed in pre-adipocytes than in fully differentiated adipocytes. In conclusion, our results provide valuable information for further understanding of the adipogenic process.
Keywords
3T3-L1; adipogenesis; differentiation; obesity; proteome; two-dimensional gel electrophoresis;
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