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Antitumor Effect of Soluble ${\beta}$-1,3-Glucan from Agrobacterium sp. R259 KCTC 1019  

Shim, Jung-Hyun (Department of Bioscience and Biotechnology, Konkuk University)
Sung, Ki-Joong (Department of Bioscience and Biotechnology, Konkuk University)
Cho, Min-Chul (Department of Bioscience and Biotechnology, Konkuk University)
Choi, Won-A (DMJ Biotech Corp.)
Yang, Young (Department of Biological Sciences, Research Center for Women's Diseases, Sookmyung Womens's University)
Lim, Jong-Seok (Department of Biological Sciences, Research Center for Women's Diseases, Sookmyung Womens's University)
Yoon, Do-Young (Department of Bioscience and Biotechnology, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.9, 2007 , pp. 1513-1520 More about this Journal
Abstract
[ ${\beta}$ ]-1,3-Glucans enhance immune reactions such as antitumor, antibacterial, antiviral, anticoagulatory, and wound healing activities. ${\beta}$-1,3-Glucans have various functions depending on the molecular weight, degree of branching, conformation, water solubility, and intermolecular association. The molecular weight of the soluble glucan was about 15,000 as determined by a high-performance size exclusion chromatography. From the infrared (IR) and $^{13}C$ NMR analytical data, the purified soluble glucan was found to exclusively consist of ${\beta}$-D-glucopyranose with 1,3 linkage. We tested the immunestimulating activities of the soluble ${\beta}$-1,3-glucan extracted from Agrobacterium sp. R259 KCTC 1019 and confirmed the following activities. IFN-$_{\gamma}$ and each cytokines were induced in the spleens and thymus of mice treated with soluble ${\beta}$-1,3-glucan. Adjuvant effect was observed on antibody production. Nitric oxide was synthesized in monocytic cell lines treated with ${\beta}$-1,3-glucan. The cytotoxic and antitumor effects were observed on various cancer cell lines and ICR mice. These results strongly suggested that this soluble ${\beta}$-1,3-glucan could be a good candidate for an immune-modulating agent.
Keywords
${\beta}$-1,3-Glucans; cytokine; adjuvant; antitumor; immune-stimulating activity;
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1 Chihara, G. 1992. Recent progress in immunopharmacology and therapeutic effects of polysaccharides. Dev. Biol. Stand. 77: 191-197
2 Di Luzio, N. 1985. Update on the immunomodulating activities of glucans. Springer Semin. Immunopathol. 8: 387-400
3 Ha, C. H., K. Lim, S. Jang, C. Yun, H. Paik, S. Kim, C. Kang, and H. Chang. 2006. Immune-enhancing alkali-soluble glucans produced by wild-type and mutant Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 16: 576-583   과학기술학회마을
4 Kim, M., K. Ryu, W. Choi, Y. Rhee, and I. Lee. 2003. Enhanced production of (1,3)-$\beta$-D-glucan by a mutant strain of Agrobacterium sp. Biochem. Eng. J. 16: 163-168   DOI   ScienceOn
5 Nemoto, J., N. Ohno, K. Saito, Y. Adachi, and T. Yadomae. 1994. Analysis of cytokine mRNAs induced by the administration of a highly branched (1-->3)-beta-D-glucan, OL-2. Biol. Pharm. Bull. 17: 948-954   DOI   ScienceOn
6 Rios-Hernandez, M., N. Dos-Santos, Silvia-Cardosa, J. Bello-Garciga, and M. Pedroso. 1994. Immunopharmacological studies of beta-1,3-glucan. Arch. Med. Res. 25: 175-180
7 Suda, M., N. Ohno, Y. Adachi, and T. Yadomae. 1995. Modulation of the antitumor effect and tissue distribution of highly branched (1->3)-beta-D-glucan, SSG, by carrageenan. Biol. Pharm. Bull. 18: 772-775   DOI   ScienceOn
8 Taguchi, T., Y. Kaneko, and G. Chihara. 1988. Lentinan. Biotherapy 2: 509
9 Tsuzuki, A., T. Tateishi, N. Ohno, Y. Adachi, and T. Yadomae. 1999. Increase of hematopoietic responses by triple or single helical conformer of an antitumor (1->3)- beta-D-glucan preparation, sonifilan, in cyclophosphamideinduced leukopenic mice. Biosci. Biotechnol. Biochem. 63: 104-110   DOI   ScienceOn
10 Hong, K., K. Jang, J. Lee, S. Kim, M. Kim, I. Lee, S. Kim, Y. Lim, and S. Kang. 2005. Bacterial $\beta$-glucan exhibits potent hypoglycemic activity via decrease of serum lipids and adiposity, and increase of UCP mRNA expression. J. Microbiol. Biotechnol. 15: 823-830   과학기술학회마을
11 Cho, Y., J. Cho, K. Lee, S. Park, and D. Yoon. 2000. Development of screening systems for drugs against human papillomavirus-associated cervical cancer: Based on E6-E6AP binding. Antiviral Res. 47: 199-206   DOI   ScienceOn
12 Sonn, C., H. Yoon, I. Seong, M. Chang, Y. Kim, H. Kang, S. Suh, and Y. Kim. 2006. MethA fibrosarcoma cells expressing membrane-bound forms of IL-2 enhance antitumor immunity. J. Microbiol. Biotechnol. 16: 1919-1927   과학기술학회마을
13 Seljeled, R., J. Bogwald, and A. Lundwall. 1981. Glycan stimulation of macrophages in vitro. Exp. Cell Res. 131: 121-129   DOI   ScienceOn
14 Cheung, N., S. Modak, A. Vickers, and B. Knuckles. 2002. Orally administered beta-glucans enhance anti-tumor effects of monoclonal antibodies. Cancer Immunol. Immunother. 51: 557-564
15 Nathan, C., H. Murray, M. Wiebe, and B. Rubin. 1983. Identification of interferon-gamma as the the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity. J. Exp. Med. 158: 670-689   DOI   ScienceOn
16 Oka, M., S. Hazama, M. Suzuki, F. Wang, and K. Wadamori. 1996. In vitro and in vivo analysis of human leukocyte binding by the antitumor polysaccharide, lentinan. Int. J. Immunopharmacol. 18: 211-216   DOI   ScienceOn
17 Tokunaka, K., N. Ohno, Y. Adachi, S. Tanaka, and H. Tamura. 2000. Immunopharmacological and immunotoxicological activities of a water-soluble (1->3)-beta-D-glucan, CSBG, from Candida spp. Int. J. Immunopharmacol. 22: 383-394   DOI   ScienceOn
18 Wasser, S. 2002. Medicinal mushrooms as a source of antitumor and immuno-modulating polysaccharides. Appl. Microbiol. Biotechnol. 60: 258-274   DOI   ScienceOn
19 Fujimoto, S. 1989. Clinical efficacies of schizophyllan (SPG) on advanced gastric cancer. Nippon Geka Gakkai Zasshi 90: 1447-1450
20 Raveh, D., B. Kruskal, J. Farland, and R. Ezekowitz. 1998. Th1 and Th2 cytokines cooperate to stimulate mannose-receptormediated phagocytosis. J. Leukocyte Biol. 64: 108-113   DOI
21 Nicola, N. and D. Hilton. 1998. General classes and functions of four-helix bundle cytokines. Adv. Protein Chem. 52: 1-65   DOI
22 Onozaki, K., K. Matsushima, E. Kleinerman, T. Saito, and J. Oppenheim. 1985. Role of interleukin-1 in promoting human monocyte-mediated tumor cytotoxicity. J. Immunol. 135: 314-320
23 Harada, T., N. Miura, Y. Adachi, M. Nakajima, T. Yadomae, and N. Ohno. 2002. IFN-gamma induction by SCG, 1,3- beta-D-glucan from Sparassis crispa, in DBA/2 mice in vitro. J. Interferon Cytokine Res. 22: 1227-1239   DOI   ScienceOn
24 Kondo, Y., A. Kato, H. Hojo, S. Nozoe, M. Takeuchi, and K. Ochi. 1992. Cytokine-related immunopotentiating activities of paramylon, a beta-(1->3)-D-glucan from Euglena gracilis. J. Pharmacobiodyn. 15: 617-621   DOI
25 Migliorini, P., G. Corradin, and S. Corradin. 1991. Macrophage $NO_2$-production as a sensitive and rapid assay for the quantitation of murine IFN-$\gamma$. J. Immunol. Methods 139: 107-114   DOI   ScienceOn
26 Williams, D., H. Pretus, R. McNamee, E. Jones, H. Ensley, I. Browder, and N. Di Luzio. 1991. Development, physicochemical characterization and preclinical efficacy evaluation of a water soluble glucan sulfate derived from Saccharomyces cerevisiae. Immunopharmacology 22: 139-155   DOI   ScienceOn
27 Ha, C. H., C. W. Yun, H. D. Paik, S. W. Kim, C. W. Kang, H. J. Hwang, and H. I. Chang. 2006. Preparation and analysis of yeast cell wall mannoproteins, immune enhancing materials, from cell wall mutant Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 16: 247-255   과학기술학회마을
28 Colle, J., P. Falanga, M. Singer, B. Hevin, and G. Milon. 1997. Quantitation of messenger RNA by competitive RTPCR: A simplified read out assay. J. Immunol. Methods 210: 175-184   DOI   ScienceOn
29 Mohagheghpour, N., M. Dawson, P. Hobbs, A. Judd, and R. Winant. 1995. Glucans as immunological adjuvants. Adv. Exp. Med. Biol. 383: 13-22
30 Bohn, J. and J. BeMiller. 1995. $(1{\rightarrow}3)-{\beta}$-Glucans as biological response modifiers: A review of structurefunctional activity relationships. Carbohydr. Polym. 28: 3-14   DOI   ScienceOn
31 Taga, T. and T. Kishimoto. 1997. GP130 and the interleukin- 6 family of cytokines. Annu. Rev. Immunol. 15: 797-819   DOI   ScienceOn
32 Reizenstein, P. and G. Mathe. 1984. Immunomodulating agent. Immunol. Ser. 35: 347
33 Onozaki, K., K. Matsushima, and B. Aggarwal. 1985. Human interleukin-1 is a cytocidal factor for several tumor cell lines. J. Immunol. 135: 3962-3968