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Inhibitory Effects of Cordycepin (3'-Deoxyadenosine), a Component of Cordyceps militaris, on Human Platelet Aggregation Induced by Thapsigargin  

Cho, Hyun-Jeong (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, and Regional Research Center, Inje University)
Cho, Jae-Youl (School of Bioscience and Biotechnology, Kangwon National University)
Rhee, Man-Hee (Department of Physiology, College of Veterinary Medicine, Kyungpook National University)
Kim, Hyeong-Soo (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, and Regional Research Center, Inje University)
Lee, Hyun-Sub (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, and Regional Research Center, Inje University)
Park, Hwa-Jin (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, and Regional Research Center, Inje University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.7, 2007 , pp. 1134-1138 More about this Journal
Abstract
Cordycepin (3'-deoxyadenosine) is an adenosine analog, isolated from Cordyceps militaris, and it has been used as an anticancer and anti-inflammation ingredient in traditional Chinese medicine. We investigated the effects of cordycepin (3'-deoxyadenosine) on human platelet aggregation, which was induced by thapsigargin, a tumor promoter, and determined the cytosolic free $Ca^{2+}$ levels ($[Ca^{2+}]_i$) (an aggregation-stimulating molecule) and cyclic-guanosine monophosphate (cGMP) (an aggregation-inhibiting molecule). Cordycepin inhibited thapsigargin-induced platelet aggregation in a dose-dependent manner, and it clearly reduced the levels of $[Ca^{2+}]_i$, which was increased by thapsigargin ($1\;{\mu}M$) or U46619 ($3\;{\mu}M$). Cordycepin also increased the thapsigargin-reduced cGMP levels. Accordingly, our data demonstrated that cordycepin may have a beneficial effect on platelet aggregation-mediated thrombotic diseases through the $[Ca^{2+}]_i$-regulating system such as cGMP.
Keywords
Cordycepin; intracellular $Ca^{2+}$; cGMP; antiplatelet activity;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 13  (Related Records In Web of Science)
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