Browse > Article

Effects of Culture Conditions on Osteogenic Differentiation in Human Mesenchymal Stem Cells  

Song, Su-Jin (Department of Chemical Engineering, Hanyang University)
Jeon, O-Ju (Department of Chemical Engineering, Hanyang University)
Yang, Hee-Seok (Department of Bioengineering, Hanyang University)
Han, Dong-Keun (Biomaterials Research Center, Korea Institute of Science and Technology)
Kim, Byung-Soo (Department of Bioengineering, Hanyang University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.7, 2007 , pp. 1113-1119 More about this Journal
Abstract
Human bone marrow-derived mesenchymal stem cells (hBMMSCs) must differentiate into osteogenic cells to allow for successful bone regeneration. In this study, we investigated the effects of different combinations of three soluble osteogenic differentiation-inducing factors [L-ascorbic acid (AC), ${\beta}$-glycerophosphate (${\beta}G$), and bone morphogenic protein-2 (BMP-2)] and the presence of a hydroxyapatite (HA) substrate on hBMMSC osteogenic differentiation in vitro. hBMMSCs were cultured in medium containing various combinations of the soluble factors on culture plates with or without HA coating. After 7 days of culture, alkaline phosphatase (ALP) activity, calcium deposition, and osteoprotegerin (OPG) and osteopontin (OPN) expression were measured. The effects of individual and combined factors were evaluated using a factorial analysis method. BMP-2 predominantly affected expression of early markers of osteogenic differentiation (ALP and OPG). HA had the highest positive effect on OPN expression and calcium deposition. The interaction between AC, ${\beta}G$, and HA had the second highest positive effect on ALP activity.
Keywords
Bone; differentiation; mesenchymal stem cell;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 9  (Related Records In Web of Science)
연도 인용수 순위
1 Ciapetti, G, L. Ambrosio, G Marietta, N. Baldini, and A. Giunti. 2006. Human bone marrow stromal cells: In vitro expansion and differentiation for bone engineering. Biomaterials 27: 6150-6160   DOI   ScienceOn
2 Fedde, K. N., L. Blair, J. Silverstein, S. P. Coburn, L. M. Ryan, R. S. Weinstein, K. Waymire, S. Narisawa, J. L. Millan, G. R. MacGregor, and M. P. Whyte. 1999. Alkaline phosphatase knock-out mice recapitulate the metabolic and skeletal defects of infantile hypophosphatasia. J. Bone Miner. Res. 14: 2015-2026   DOI   ScienceOn
3 Jagodzinski, M., M. Drescher, J. Zeichen, S. Hankemeier, C. Krettek, U. Bosch, and M. van Griensven. 2004. Effects of cyclic longitudinal mechanical strain and dexamethasone on osteogenic differentiation of human bone marrow stromal cells. Eur. Cell. Mater. 7: 35-41   DOI
4 Jaiswal, N., S. E. Haynesworth, A. I. Caplan, and S. P. Bruder. 1997. Osteogenic differentiation of purified, cultureexpanded human mesenchymal stem cells in vitro. J. Cell. Bioehem. 64: 295-312   DOI   ScienceOn
5 Kotobuki, N., K. Ioku, D. Kawagoe, H. Fujimori, S. Goto, and H. Ohgushi. 2005. Observation of osteogenic differentiation cascade of living mesenchymal stem cells on transparent hydroxyapatite ceramics. Biomaterials 26: 779-785   DOI   ScienceOn
6 Park, J. H., S. R. Yu, J. S. Yoon, and K. H. Baek. 2005. Highlevel expression of recombinant human bone morphogenetic protein-4 in Chinese hamster ovary cells. J. Microbiol. Biotechnol. 15: 1397-1401   과학기술학회마을
7 Riddle, R. C., A. F. Taylor, D. C. Genetos, and H. J. Donahue. 2006. MAP kinase and calcium signaling mediate fluid flow-induced human mesenchymal stem cell proliferation. Am. J. Physiol. Cell Physiol. 290: 776-784   DOI
8 Yamaguchi, A., T. Ishizuya, and N. Kintou. 1996. Effects of BMP-2, BMP-4, and BMP-6 on osteoblastic differentiation of bone marrow-derived stromal cell lines, ST2 and MC3T3G2/PA6. Biochem. Biophys Res. Commun. 220: 366-371   DOI   ScienceOn
9 Zhang, R. and P. X. Ma. 2004. Biomimetic polymer/apatite composite scaffolds for mineralized tissue engineering. Macromol. Biosci. 4: 100-111   DOI   ScienceOn
10 Brugge, T. P. and J. A. Jansen. 2002. In vitro osteogenic differentiation of rat bone marrow cells subcultured with and without dexamethasone. Tissue Eng. 8: 321-331   DOI   ScienceOn
11 Song, H. B., D. C. Park, G. M. Do, S. L. Hwang, W. K. Lee, H. S. Kang, B. R. Park, H. J. Jang, C. W. Son, E. K. Park, S. Y. Kim, and T. L. Huh. 2006. Effect of exopolymers of Aureobasidium pullulans on improving osteoporosis induced in ovariectomized mice. J. Microbiol. Biotechnol. 16: 37-45   과학기술학회마을
12 Yang, H. S., G D. Park, K. D. Ahn, B. S. Kim, and D. K. Han. 2006. Optimal hydrophilization and chondrocyte adhesion of PLLA and scaffolds by plasma treatment and acrylic acid grafting. Polymer (Korea) 30: 168-174   과학기술학회마을
13 Haynesworth, S. E., J. Goshinia, V. M. Goldberg, and A. I. Caplan. 1992. Characterization of cells with osteogenic potential from human marrow. Bone 13: 81-88   DOI   ScienceOn
14 Mendes, S. C., I. Van Den Brink, J. D. De Bruijn, and C. A. Van Blitterswijk. 1998. In vivo bone formation by human bone marrow cells: Effect of osteogenic culture supplements and cell densities. J. Mater. Sci. Mater. Med. 9: 855-858   DOI   ScienceOn
15 Poliard, A., D. Lamblin, P. J. Marie, M. H. Buc-Caron, and O. Kellermann. 1993. Commitment of the teratocarcinomaderived mesodermal clone C1 towards terminal osteogenic differentiation. J. Cell Sci. 106: 503-512
16 Shukunami, C., K. Ishizeki, T. Atsumi, Y. Ohta, F. Suzuki, and Y. Hiraki. 1997. Cellular hypertrophy and calcification of embryonal carcinoma-derived chondrogenic cell line ATDC5 in vitro. J. Bone Miner. Res. 12: 1174-1188   DOI   ScienceOn
17 Pittenger, M. F., A. M. Mackay, S. C. Beck, R. K. Jaiswal, R. Douglas, J. D. Mosca, M. A. Moorman, D. W. Simonetti, S. Craig, and D. R. Marshak. 1999. Multilineage potential of adult human mesenchymal stem cells. Science 284: 143-147   DOI
18 Choong, P. F. M., T. J. Martin, and K. W. Ng. 1993. Effects of ascorbic acid, calcitriol, and retinotic acid on the differentiation ofpreosteoblasts. J. Orthop. Res. 11: 638-647   DOI   ScienceOn
19 Kim, M. K. and C. Niyibizi. 2001. Interaction of TGF-betal and rhBMP-2 on human bone marrow stromal cells cultured in collagen gel matrix. Yonsei Med. J. 42: 338-344   DOI
20 Gomes, M. E., H. L. Holtorf, R. L. Reis, and A. G. Mikos. 2006. Influence of the porosity of starch-based fiber mesh scaffolds on the proliferation and osteogenic differentiation of bone marrow stromal cells cultured in a flow perfusion bioreactor. Tissue Eng. 12: 801-809   DOI   ScienceOn
21 Hofinann, S., H. Hagenmuller, A. M. Koch, R. Muller, G. Vunjak-Novakovic, D. L. Kaplan, H. P. Merkle, and L. Meinel. 2007. Control of in vitro tissue-engineered bone-like structures using human mesenchymal stem cells and porous silk scaffolds. Biomaterials 28: 1152-1162   DOI   ScienceOn
22 Agrawal, M. K., S. K. Ghosh, D. Bagchi, J. Weckesser, M. Erhard, and S. N. Bagchi. 2006. Occurrence of microcyst incontaining toxic water blooms in central India. J. Microbiol. Biotechnol. 16: 212-218   과학기술학회마을
23 Hessle, L., K. A. Johnson, H. C. Anderson, S. Narisawa, A. Sali, J. W. Goding, R. Terkeltaub, and J. L. Millan. 2002. Tissue-nonspecific alkaline phosphatase and plasma cell membrane glycoprotein-1 are central antagonistic regulators of bone mineralization. Proc. Natl. Aead. Sci. USA 99: 9445-9449
24 Maniatopoulos, C., J. Sodek, and A. Melcher. 1988. Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell Tissue Res. 254: 317-330
25 Anderson, H. C., J. B. Sipe, L. Hessle, R. Dhanyamrctiu, E. Atti, N. P. Camacho, and J. L. Millan. 2004. Impaired calcification around matrix vesicles of growth plate and bone in alkaline phosphatase-deficient mice. Am. J. Pathol. 164: 841-847   DOI   ScienceOn
26 Kim, S. S., M. S. Park, O. Jeon, C. Y. Choi, and B. S. Kim. 2006. Poly(lactide-co-glycolide)/hydroxyapatite composite scaffolds for bone tissue engineering. Biomaterials 27: 1399-1409   DOI   ScienceOn
27 Osyczka, A. M. and P. S. Leboy. 2005. Bone morphogenetic protein regulation of early osteoblast genes in human marrow stromal cells is mediated by extracellular signalregulated kinase and phosphatidylinositol 3-kinase signaling. Endocrinology 146: 3428-3437   DOI   ScienceOn
28 Chen, Y. L., H. C. Chen, H. P. Lee, H. Y. Chan, and Y. C. Hu. 2006. Rational development of GAG-augmented chitosan membranes by fractional factorial design methodology. Biomaterials 27: 2222-2232   DOI   ScienceOn
29 Centrella, M., M. C. Horowitz, J. M. Wozney, and T. L. McCarthy. 1994. Transforming growth factor-beta gene family members and bone. Endocr. Rev. 15: 27-39
30 Pereira, R. C., A. M. Delany, and E. Canalis. 2002. Effects of cortisol and bone morphogenetic protein-2 on stromal cell differentiation: Correlation with CCAAT-enhancer binding protein expression. Bone 30: 685-691   DOI   ScienceOn
31 Yoneno, K., S. Ohno, K. Tanimoto, K. Honda, N. Tanaka, T. Doi, T. Kawata, E. Tanaka, S. Kapila, and K. Tanne. 2005. Multidifferentiation potential of mesenchymal stem cells in three-dimensional collagen gel cultures. J. Biomed. Mater. Res. A 75: 733-741
32 Yamamoto, N., K. Furuya, and K. Hanada. 2002. Progressive development of the osteoblast phenotype during differentiation of osteoprogenitor cells derived from fetal rat calvaria: Model for in vitro bone formation. Biol. Pharm. Bull. 25: 509-515   DOI   ScienceOn
33 Cheng, S. L., J. W. Yang, L. Rifas, S. F. Zhang, and L. V. Avioli. 1994. Differentiation of human bone marrow osteogenic stromal cells in vitro: Induction of the osteoblast phenotype by dexamethasone. Endocrinology 134: 277-286   DOI   ScienceOn
34 Jager, M., T. Feser, H. Denck, and R. Krauspe. 2005. Proliferation and osteogenic differentiation of mesenchymal stem cells cultured onto three different polymers in vitro. Ann. Biomed. Eng. 33: 1319-1332   DOI   ScienceOn
35 Lecanda, F., L. V. Avioli, and S. L. Cheng. 1997. Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2. J. Cell. Biochem. 67: 386-396   DOI   ScienceOn
36 Zhao, F., R. Chell a, and T. Ma. 2006. Effects of shear stress on 3-D human mesenchymal stem cell construct development in a perfusion bioreactor system: Experiments and hydrodynamic modeling. Biotechnol. Bioeng. 96: 584-595
37 Kasten, P., J. Vogel, R. Luginbuhl, P. Niemeyer, S. Weiss, S. Schneider, M. Kramer, A. Leo, and W. Richter. 2006. Influence of platelet-rich plasma on osteogenic differentiation of mesenchymal stem cells and ectopic bone formation in calcium phosphate ceramics. Cells Tissues Organs 183: 68-79   DOI   ScienceOn
38 Chou, Y. F., W. Huang, J. C. Dunn, T. A. Miller, and B. M. Wu. 2005. The effect of biomimetic apatite structure on osteoblast viability, proliferation, and gene expression. Biomaterials 26: 285-295   DOI   ScienceOn
39 Kim, S. S., M. S. Park, S. J. Gwak, C. Y. Choi, and B. S. Kim. 2006. Accelerated bonelike apatite growth on porous polymer/ceramic composite scaffolds in vitro. Tissue Eng. 12: 2997-3006   DOI   ScienceOn
40 Mauney, J. R., S. Sjostorm, J. Blumberg, R. Horan, J. P. O'Leary, G. Vunjak-Novakovic, V. Volloch, and D. L. Kaplan. 2004. Mechanical stimulation promotes osteogenic differentiation of human bone marrow stromal cells on 3-D partially demineralized bone scaffolds in vitro. Calcif. Tissue Int. 74: 458-468   DOI   ScienceOn
41 Simmons, C. A., S. Matlis, A. J. Thornton, S. Chen, C. Y. Wang, and D. J. Mooney. 2003. Cyclic strain enhances matrix mineralization by adult human mesenchymal stem cells via the extracellular signal-regulated kinase (ERK 1/2) signaling pathway. J. Biomech. 36: 1087-1096   DOI   ScienceOn
42 Ito, C., W. E. Evans, L. McNinch, E. Coustan-Smith, H. Mahmoud, C. H. Pui, and D. Campana. 1996. Comparative cytotoxicity of dexamethasone and prednisolone in childhood acute lymphoblastic leukemia. J. Clin. Oncol. 14: 2370-2376   DOI