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Cloning of Fibrinolytic Enzyme Gene from Bacillus subtilis Isolated from Cheonggukjang and Its Expression in Protease-deficient Bacillus subtilis Strains  

Jeong, Seon-Ju (Division of Applied Life Science, Graduate School, Gyeongsang National University)
Kwon, Gun-Hee (Division of Applied Life Science, Graduate School, Gyeongsang National University)
Chun, Ji-Yeon (Division of Applied Life Science, Graduate School, Gyeongsang National University)
Kim, Jong-Sang (Department of Animal Biotechnology, Kyungpook National University)
Park, Cheon-Seok (Department of Food Science & Biotechnology, Kyung Hee University)
Kwon, Dae-Young (Food Function Research Division, Korea Food Research Institute)
Kim, Jeong-Hwan (Institute of Agriculture & Life Science, Gyeongsang National University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.6, 2007 , pp. 1018-1023 More about this Journal
Abstract
Bacillus subtilis CH3-5 was isolated from cheonggukjang prepared according to traditional methods. CH3-5 secreted at least four different fibrinolytic proteases (63, 47, 29, and 20 kDa) into the culture medium. A fibrinolytic enzyme gene, aprE2, encoding a 29kDa enzyme was cloned from the genomic DNA of CH3-5, and the DNA sequence determined. aprE2 was overexpressed in heterologous B. subtilis strains deficient in extracellular proteases using a E. coli-Bacillus shuttle vector. A 29 kDa AprE2 band was observed and AprE2 seemed to exhibit higher activities towards fibrin rather than casein.
Keywords
Cheonggukjang; fibrinolytic enzyme; Bacillus subtilis; gene expression;
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Times Cited By KSCI : 6  (Citation Analysis)
Times Cited By Web Of Science : 13  (Related Records In Web of Science)
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