Cloning of Fibrinolytic Enzyme Gene from Bacillus subtilis Isolated from Cheonggukjang and Its Expression in Protease-deficient Bacillus subtilis Strains
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Jeong, Seon-Ju
(Division of Applied Life Science, Graduate School, Gyeongsang National University)
Kwon, Gun-Hee (Division of Applied Life Science, Graduate School, Gyeongsang National University) Chun, Ji-Yeon (Division of Applied Life Science, Graduate School, Gyeongsang National University) Kim, Jong-Sang (Department of Animal Biotechnology, Kyungpook National University) Park, Cheon-Seok (Department of Food Science & Biotechnology, Kyung Hee University) Kwon, Dae-Young (Food Function Research Division, Korea Food Research Institute) Kim, Jeong-Hwan (Institute of Agriculture & Life Science, Gyeongsang National University) |
1 | Choi, N. S., K. T. Chang, P. J. Maeng, and S. H. Kim. 2004. Cloning, expression, and fibrin (ogen)olytic properties of a subtilisin DJ-4 gene from Bacillus sp. DJ-4. FEMS Microbiol. Lett. 236: 325-331 DOI ScienceOn |
2 | Lee, H.-S., C.-K. Yoo, C.-S. Lee, and S. M. Kang. 2000. Variation of fibrinolytic enzyme activity produced from Bacillus subtilis by gene cloning. Kor. J. Appl. Microbiol. Biotechnol. 28: 14-20 |
3 | Yoo, C.-K., W.-S. Seo, C.-S. Lee, and S. M. Kang. 1998. Purification and characterization of fibrinolytic enzyme excreted by Bacillus subtilis K-54 isolated from Chung Guk Jang. Kor. J. Appl. Microbiol. Biotechnol. 26: 507-514 |
4 | Ahn, Y.-S., Y.-S. Kim, and D.-H. Shin. 2006. Isolation, identification, and fermentation characteristics of Bacillus sp. with high protease activity from traditional Cheonggukjang. Kor. J. Food Sci. Technol. 38: 82-87 과학기술학회마을 |
5 | Wu, X. C., W. Lee, L. Tran, and S. L. Wong. 1991. Engineering a Bacillus subtilis expression-secretion system synthesis with a strain deficient in six extracellular proteases. J. Bacteriol. 173: 4952-4958 DOI |
6 | Xu, D. and J.-C. Cote. 2003. Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3' end 16S rDNA and 5' end 16S-23S ITS nucleotide sequences. Int. J. Syst. Evol. Microbiol. 53: 695- 704 DOI ScienceOn |
7 | Sambrook, J. and D. W. Russel. 2001. Molecular Cloning: A Laboratory Manual, 3rd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, U.S.A |
8 | Chang, J. H., Y. Y. Shim, S. H. Kim, K. M. Chee, and S. K. Cha. 2005. Fibrinolytic and immunostimulating activities of Bacillus spp. strains isolated from Chungkuk-jang. Kor. J. Food Sci. Technol. 37: 255-260 과학기술학회마을 |
9 | Paik, H.-D., S.-K. Lee, S. Heo, S.-Y. Kim, H.-H. Lee, and T. J. Kwon. 2004. Purification and characterization of the fibrinolytic enzyme produced by Bacillus subtilis KCK-7 from Chungkookjang. J. Microbiol. Biotechnol. 14: 829- 835 |
10 | Jang, J. S., D. O. Kang, M. J. Chun, and S. M. Byun. 1992. Molecular cloning of a subtilisin J gene from Bacillus stearothermophilus and its expression in Bacillus subtilis. Biochem. Biophys. Res. Commun. 184: 277-282 DOI ScienceOn |
11 | Coligan, J. E., B. M. Dunn, D. W. Speicher, and P. T. Wingfield (eds.). 2003. Short Protocols in Protein Science. pp. 3-31-3-32 |
12 | Lee, J.-O., S.-D. Ha, A. J. Kim, C. S. Yuh, I.-S. Bang, and S. H. Park. 2005. Industrial application and physiological functions of Chongkukjang. Food Sci. Indus. 38: 69-78 |
13 | Peng, Y., X. Yang, and Y. Zhang. 2005. Microbial fibrinolytic enzymes: An overview of source, production, properties, and thrombolytic activity in vivo. Appl. Microbiol. Biotechnol. 69: 126-132 DOI ScienceOn |
14 | Jeong, S. J., J. Y. Park, J. H. Kim, G. M. Kim, J. Y. Chun, J. H. Lee, D. K. Chung, and J. H. Kim. 2006. Transformation of Leuconostoc mesenteroides SY1, a strain isolated from Kimchi. J. Microbiol. Biotechnol. 16: 149-152 과학기술학회마을 |
15 | Nakamura, T., Y. Yamagata, and E. Ichishima. 1992. Nucleotide sequence of the subtilisin NAT gene, aprN, of Bacillus subtilis (Natto). Biosci. Biotech. Biochem. 56: 1869-1871 DOI ScienceOn |
16 |
Park, S.-S., S.-L. Wong, L.-F. Wang, and R. H. Doi. 1989. Bacillus subtilis subtilisin gene (aprE) is expressed from a |
17 | Bradford, M. M. 1976. Rapid and sensitive methods for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254 DOI ScienceOn |
18 | Choi, N. S., K. H. Yoo, J. H. Hahm, K. S. Yoon, K. T. Chang, B. H. Hyun, P. J. Maeng, and S. H. Kim. 2005. Purification and characterization of a new peptidase, bacillopeptidase DJ-2, having fibrinolytic activity produced by Bacillus sp. DJ-2 from Doen-jang. J. Microbiol. Biotechnol. 15: 72-79 과학기술학회마을 |
19 | Kim, S.-H., J.-L. Yang, and Y. S. Song. 1999. Physiological functions of Chongkukjang. Food Ind. Nutr. 4: 40-46 |
20 | Choi, N. S., D.-M. Chung, C. H. Ryu, K.-S. Yoon, P. J. Maeng, and S. H. Kim. 2006. Identification of three extracellular proteases from Bacillus subtilis KCTC 3014. J. Microbiol. Biotechnol. 16: 457-464 과학기술학회마을 |
21 | Nakamura, H., H. Takagi, and M. Inouye. 1987. Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli. J. Biol. Chem. 262: 7859-7864 |
22 | Lee, S.-K., D.-H. Bae, T. J. Kwon, S. B. Lee, H.-H. Lee, J. H. Park, S. Heo, and M. G. Johnson. 2001. Purification and characterization of a fibrinolytic enzyme from Bacillus sp. KDO-13 isolated from soybean paste. J. Microbiol. Biotechnol. 11: 845-852 |
23 | Noh, K. A., D.-H. Kim, N.-S. Choi, and S.-H. Kim. 1999. Isolation of fibrinolytic enzyme producing strain from Kimchi. Kor. J. Food Sci. Technol. 31: 219-223 |
24 | Kim, W. K., K.-H. Choi, Y.-T. Kim, H.-H. Park, J.-Y. Choi, Y.-S. Lee, H.-I. Oh, I.-B. Kwon, and S. Y. Lee. 1996. Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkook-Jang. Appl. Environ. Microbiol. 62: 2482-2488 |
25 | Kurihara, M., F. S. Markland, and E. L. Smith. 1972. Subtilisin amylosacchariticus. 3. Isolation and sequence of the chymotryptic peptides and the complete amino acid sequence. J. Biol. Chem. 247: 5619-5631 |
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