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Detection of Methicillin Resistance in Staphylococcus aureus Isolates Using Two-Step Triplex PCR and Conventional Methods  

Cho, Joon-Il (Department of Food Science and Technology, Chung-Ang University)
Jung, Hye-Jin (Department of Food Science and Technology, Chung-Ang University)
Kim, Young-Joon (Department of Food Science and Technology, Chung-Ang University)
Park, Sung-Hee (Department of Food Science and Technology, Chung-Ang University)
Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University)
Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.4, 2007 , pp. 673-676 More about this Journal
Abstract
A two-step triplex PCR assay targeting the mecA, femA, and nuc genes was developed for the detection of methicillin resistance genes harbored by some Staphylococcus aureus isolates and for the simultaneous identification of such isolates at the species level. The triplex PCR revealed the presence of the femA and nuc genes in all the S. aureus isolates examined (n=105). Forty-four clinical isolates were mecA positive and no foodborne isolates were mecA positive. The PCR results had a 98 or 99% correlation with the results of PBP2a latex agglutination tests or oxacillin susceptibility tests, respectively.
Keywords
Two-step triplex PCR; methicillin-resistant S. aureus (MRSA); mecA gene; femA gene; nuc gene;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
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