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Accurate Delimitation of Phanerochaete chrysosporium and Phanerochaete sordida by Specific PCR Primers and Cultural Approach  

Lim, Young-Woon (Institute of Molecular Biology and Genetics, Seoul National University)
Baik, Keun-Sik (Department of Biological Sciences, College of Natural Science, Sunchon National University)
Chun, Jong-Sik (School of Biological Sciences and Institute of Microbiology, Seoul National University)
Lee, Kang-Hyun (Insect Resources Research Center, KRIBB)
Jung, Won-Jin (Insect Resources Research Center, KRIBB)
Bae, Kyung-Sook (Insect Resources Research Center, KRIBB)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.3, 2007 , pp. 468-473 More about this Journal
Abstract
White rot fungi, Phanerochaete chrysosporium and Phanerochaete sordida, have been mostly studied in a variety of industrial processes like biopulping and pulp bleaching as well as in bioremediation. Whereas P. sordida is widely distributed in the North Temperate Zone, P. chrysosporium is reported in the restricted area and hundreds of reports have been described from a few strains of P. chrysosporium, which are deposited at various fungal collections in the world. The isolates of two species are not easily discriminated because of their morphological and molecular similarity. Through the ITS sequence analyses, a region containing substantial genetic variation between the two species was identified. PCR amplification using two specific primers was successfully used to differentiate P. chrysosporium from P. sordida. These results were supported by cultural studies. The growth rates at $37^{\circ}C$ on PDA, MEA, and Cza and the microscopic features of conidia on PDA and YMA were also very useful to differentiate those two species.
Keywords
Phanerochaete chrysosporium; Phanerochaete sordida; specific primer; cultural approach;
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Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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1 Aust, S. D. 1990. Degradation of environmental pollutants by Phanerochaete chrysosporium. Microbiol. Ecol. 20: 197- 209   DOI
2 Burdsall, H. H. and W. E. Eslyn. 1974. A new Phanerochaete with a chysosporium imperfect state. Mycotaxon 1: 123- 133
3 Choi, W.-Y., S.-O. Kim, J.-H. Lee, J.-M. Lee, I.-J. Lee, K.-J. Cho, I.-K. Rhee, J.-B. Kwon, and J.-G. Kim. 2005. Isolation of gibberellins-producing fungi from the root of several Sesamum indicum plants. J. Microbiol. Biotechnol. 15: 22- 28   과학기술학회마을
4 Eslyn, W. E. 1967. Outside storage of hardwood chips in the Northeast. II. Microbiological effects. Tappi 50: 297-303
5 Garzillo, A. M., M. C. Colao, C. Caruso, C. Caporale, D. Celletti, and V. Buonocore. 1998. Laccase from the white-rot fungus Trametes trogii. Appl. Microbiol. Biotechnol. 49: 545-551   DOI   ScienceOn
6 Jones, D. and A. K. Bej. 1994. Detection of flood-borne microbial pathogens using polymerase chain reaction methods, pp. 341-365. In Griffin, H. G. and A. M. Griffin (eds.), PCR Technology, Current Innovations. CRC Press, London, U.K
7 Lamar, R. T., M. J. Larsen, and T. K. Kirk. 1990. Sensitivity to and degradation of pentachlorophenol by Phanerochaete spp. Appl. Environ. Microbiol. 56: 3519-3526
8 Lim, Y. W. 2001. Systematic study of corticioid fungi based on molecular sequence analyses. PhD Dissertation. Seoul National University, South Korea
9 Willits, D. A. and J. E. Sherwood. 1998. Polymerase chain reaction detection of Ustilago hordei in leaves of susceptible and resistant barley varieties. Phytopathology 89: 212-217   DOI   ScienceOn
10 Kondo, R., K. Kurashiki, and K. Sakai. 1994. In vitro bleaching of hardwood kraft pulp by extracellular enzymes excreted from white rot fungi in a cultivation system using a membrane filter. Appl. Environ. Microbiol. 60: 921-926
11 White, T. J., T. D. Bruns, S. B. Lee, and J. W. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315-322. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White (eds.), PCR Protocols: A Guide to Methods and Application. Academic Press, San Diego, U.S.A
12 Lim, Y. W., Y. H. Kim, and H. S. Jung. 2000. The Aphyllophorales of Mungyong Saejae. Mycobiology 28: 142-148   DOI
13 Chen, W., L. E. Gray, and C. R. Grau. 1996. Molecular differentiation of fungi associated with brown stem rot and detection of Phialophora gregata in resistant and susceptible soybean cultivars. Phytopathology 86: 1140-1148   DOI   ScienceOn
14 Germain, H., G. Laflamme, L. Bernier, B. Boulet, and R. C. Hamelin. 2002. DNA polymorphism and molecular diagnosis in Inonotus spp. Can. J. Plant Pathol. 24: 194-199   DOI   ScienceOn
15 Lamar, R. T., J. A. Glaser, and T. K. Kirk. 1992. White rot fungi in the treatment of hazardous chemicals and wastes, pp. 127-143. In G. F. Leatham (ed.), Frontiers in Industrial Mycology. Chapman & Hall, New York
16 Rajakumar, S., J. Gaskell, D. Cullen, S. Lobos, E. Karahanian, and R. Vicuna. 1996. Lip-like genes in Phanerochaete sordida and Ceriporiopsis subermispora, white rot fungi with no detectable lignin peroxidase activity. Appl. Environ. Microbiol. 62: 2660-2663
17 Ruttimann-Johnson, C., D. Cullen, and R. T. Lamar. 1994. Manganese peroxidase of the white rot fungus Phanerochaete sordida. Appl. Environ. Microbiol. 60: 599-605
18 Boidin, J., J. Mugnier, and R. Canales. 1998. Taxonomie moleculaire des Aphyllophorales. Mycotaxon 66: 445-491
19 Dietrich, D. M. and R. T. Lamar. 1990. Selective medium for isolating Phanerochaete chrysosporium from soil. Appl. Environ. Microbiol. 56: 3088-3092
20 Lindgren, R. M. and W. E. Eslyn. 1961. Biological deterioration of pulpwood and pulp chips during storage. Tappi 44: 419-429
21 Chung, N.-H., G.-Y. Kang, G.-H. Kim, I.-S. Lee, and W.-G. Bang. 2001. Effect of nutrient nitrogen on the degradation of pentachlorophenol by white rot fungus, Phanerochaete chrysosporium. J. Microbiol. Biotechnol. 11: 704-708
22 Novotny, C., P. Erbanova, T. Cajthamal, N. Rothschild, C. Dosoretz, and V. Sasek. 2000. Irpex lacteus, a white rot fungus applicable to water and soil bioremediation. Appl. Microbiol. Biotechnol. 54: 850-853   DOI   ScienceOn
23 Bumpus, J. A., M. Tien, D. Wright, and S. D. Aust. 1985. Oxidation of persistent environmental pollutants by a white rot fungus. Science 228: 1434-1436   DOI
24 Davis, M. W., J. A. Glaser, J. W. Evans, and R. T. Lamar. 1993. Field evaluation of the lignin-degrading fungus Phanerochaete sordida to treat creosote-contaminated soil. Environ. Sci. Technol. 27: 2572-2576   DOI
25 Jeong, W. J., Y. W. Lim, J. S. Lee, and H. S. Jung. 2005. Phylogeny of Phellinus and related genera inferred from combined data of ITS and mitochondrial SSU rDNA sequences. J. Microbiol. Biotechnol. 15: 1028-1038   과학기술학회마을
26 Kang, S. W., S. I. Hong, and S. W. Kim. 2005. Identification of Aspergillus strain with antifungal activity against Phytophthora species. J. Microbiol. Biotechnol. 15: 227- 233   과학기술학회마을
27 Hunt, R. S. and F. W. Cobb. 1971. Selective medium for the isolation of wood rotting basidiomycetes. Can. J. Bot. 49: 2064-2065   DOI
28 Bahnweg, G., E. M. Möller, S. Anegg, C. Langebartels, O. Wienhaus, and H. Sandermann. 2002. Detection of Heterobasidion annosum s.l. [(Fr.) Bref.] in Norway spruce by polymerase chain reaction. J. Phytopathol. 150: 382- 389   DOI   ScienceOn
29 Harazono, K., R. Kondo, and K. Sakai. 1996. Bleaching of hardwood kraft pulp with manganese peroxidase from Phanerochaete sordida YK-624 without addition of $MnSO_{4}$. Appl. Environ. Microbiol. 62: 913-917
30 Eriksson, J., K. Hjortstam, and L. Ryvarden. 1978. The Corticiaceae of North Europe. Vol. 5. Fungiflora. Oslo, Norway
31 Lamar, R. T. 1992. The role of fungal lignin-degrading enzymes in xenobiotic degradation. Curr. Opin. Biotechnol. 3: 261-266   DOI   ScienceOn
32 Burdsall, H. H. 1985. A contribution to the taxonomy of the genus Phanerochaete (Corticiaceae, Aphyllophorales). Mycologia Memoir 10: 1-165
33 Hibbett, D. S. and R. G. Thorn. 2001. Basidiomycota: Homobasidiomycetes. In McLaughlin, D. J., McLaughlin, E. G. and Lemke, P. A. (eds.), The Mycota. Vol. VII. Systematics and Evolution. Berlin, Germany, Springer- Verlag
34 Zhao, J., T. H. Koker, and B. J. H. Janse. 1995. First report of the white rotting fungus Phanerochaete chrysosporium in South Africa. S. Afri. J. Bot. 61: 167-168   DOI
35 Gardes, M. and T. D. Bruns. 1993. ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Mol. Ecol. 2: 113- 118   DOI   ScienceOn
36 Martinez, D., L. F. Larrondo, N. Putnam, S. M. D. Gelpke, K. Huang, J. Chapman, K. G. Helfenbein, P. Ramaiya, J. C. Detter, F. Larimer, P. M. Coutinho, B. Henrissat, R. Berka, D. Cullen, and D. Rokhsar. 2004. Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78. Nature Biotechnol. 22: 695-700   DOI   ScienceOn
37 Zhang, X.-Z., H.-Y. Kim, and B.-S. Kim. 2006. Analysis of genetic diversity of Phytophthora infestans in Korea by using molecular markers. J. Microbiol. Biotechnol. 16: 423- 430   과학기술학회마을
38 Hatakka, A. 1994. Lignin-modifying enzymes from selected white-rot fungi: Production and role in lignin degradation. FEMS Microbiol. Rev. 13: 125-135   DOI   ScienceOn
39 Johnston, C. G. and S. D. Aust. 1994. Detection of Phanerochaete chrysosporium in soil by PCR and restriction enzyme analysis. Appl. Environ. Microbiol. 60: 2350-2354
40 Tien, M. and T. D. Kirk. 1983. Lignin-degrading enzyme from the hymenomycetes Phanerochaete chrysosporium Burds. Science 221: 661-663   DOI   ScienceOn
41 Lamar, R. T. and D. M. Dietrich. 1990. In situ depletion of pentachloro-phenol from contaminated soil by Phanerochaete spp. Appl. Environ. Microbiol. 56: 3039-3100