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Identification and Molecular Characterization of Novel cry1-Type Toxin Genes from Bacillus thuringiensis K1 Isolated in Korea  

Li Ming Shun (State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University)
Choi Jae-Young (Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University)
Roh Jong-Yul (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Shim Hee-Jin (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Kang Joong-Nam (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Kim Yang-Su (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Wang Yong (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Yu Zi Niu (State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University)
Jin Byung-Rae (Division of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Je Yeon-Ho (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.1, 2007 , pp. 15-20 More about this Journal
Abstract
To clone novel cry1-type genes from the Bacillus thuringiensis K1 isolate, about 2.4-kb-long PCR fragments were amplified with two primer sets of ATG1-F/N400-R and 1BeATG1-F/N400-R. Using PCR-RFLP, three novel cry1-type genes, cry1-1, cry1-7, and cry1-44, were obtained from B. thuringiensis K1 and the complete coding sequences of these novel genes were analyzed. The Cry1-1, Cry1-7, and Cry1-44 proteins showed maximum similarities of about 78.0%, 99.7%, and 91.0% with the Cry1Ha1, Cry1Be1, and Cry1Ac2 proteins, respectively. These novel cry1-type genes were expressed using a baculovirus expression vector system and their insecticidal activities were investigated. Whereas all three novel genes were toxic to Plutella xylostella larvae, only Cry1-1 showed insecticidal activity against Spodoptera exigua larvae.
Keywords
Bacillus thuringiensis K1; RFLP; cry1-type gene; inverse PCR; insecticidal activity; baculovirus expression vector system;
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Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
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