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Expression of ${\alpha}$-Galactosidase Gene from Leuconostoc mesenteroides SY1 in Leuconostoc citreum  

Park, Jae-Yong (Institute of Argriculture and Life Science, Gyeongsang National University)
Jeong, Seon-Ju (Institute of Argriculture and Life Science, Gyeongsang National University)
Lee, Ae-Ran (Division of Applied Life Science, Graduate School, Gyeongsang National University)
Park, Ji-Yeong (Division of Applied Life Science, Graduate School, Gyeongsang National University)
Jeong, Woo-Ju (Division of Applied Life Science, Graduate School, Gyeongsang National University)
Kim, Jeong-Hwan (Institute of Argriculture and Life Science, Gyeongsang National University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.12, 2007 , pp. 2081-2084 More about this Journal
Abstract
A 2.5 kb aga gene encoding ${\alpha}$-galactosidase (${\alpha}$-Gal) from Leuconostoc mesenteroides SY1 was cloned into pSJE, an E. coli-Leuconostoc shuttle vector. The recombinant plasmid, pSJEaga, was introduced into Leuconostoc citreum KCTC3526 (ATCC49370) by electroporation. Transcription level of aga was the highest in cells grown on raffinose (1%, w/v) followed by cells grown on galactose, melibiose, fructose, glucose, and sucrose. Western blot using antibodies against ${\alpha}$-Gal showed similar results to slot-blot results and enzyme activity measurements. All the results indicated that the aga was successfully expressed in L. citreum and its transcription was under the carbon catabolite repression (CCR).
Keywords
${\alpha}$-Galactosidase; gene expression; Leuconostoc citreum; CCR;
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