Changes in Aurantio-Obtusin and Glucoaurantio-Obtusin Content in Cassiae Semen via Treatment with a Crude Enzyme Extract from Aspergillus usamii

Cassiae Semen (seeds of Cassia tora) showed a remarkably different HPLC chromatogram after being treated with a crude enzyme extract from Aspergillus usamii. Increased and decreased compounds were identified as aurantio-obtusin and glucoaurantio-obtusin, respectively. The aurantio-obtusin content reached its maximum level ($133.58{\pm}0.39\;{\mu}g/mg$ extract) after being incubated for 50 min at $37^{\circ}C$, whereas the inactivated crude enzyme-treated control remained unchanged ($54.13{\pm}1.33\;{\mu}g/mg$). On the other hand, the glucoaurantio-obtusin content decreased by less than one-third ($51.09{\pm}1.63\;{\mu}g/mg$) ofthe untreated control ($143.19{\pm}2.12\;{\mu}g/mg$), suggesting that an increase in aurantio-obtusin content originated from the enzymatic cleavage of its glucoside glucoaurantio-obtusin.