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Study of Cell-mediated Response in Mice by HPV16 L1 Virus-like Particles Expressed in Saccharomyces cerevisiae  

Woo, Mi-Kyung (College of Pharmacy, Chung Ang University)
Hur, Sook-Jin (Korea Food and Drug Administration)
Park, Sue-NIe (National Institute of Toxicological Research)
Kim, Hong-Jin (College of Pharmacy, Chung Ang University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.10, 2007 , pp. 1738-1741 More about this Journal
Abstract
The first vaccine against human papillomaviruses (HPV) formulated with HPV16 L1 virus-like particles (VLPs) produced in yeast was approved by the FDA in June 2006. Nevertheless, there have been few studies of the immunogenicity in mice of VLPs. In this study, we evaluated the cell-mediated immune response to VLPs produced in Saccharomyces cerevisiae. After immunization of mice with HPV16 L1 VLPs, we measured splenocytes proliferation and the levels of IFN$_{\gamma}$, IL2, IL4, and IL5. Splenocytes proliferation was significantly increased and a mixed Th1/Th2 response was indicated. IgG subtype immunoresponses were strongly induced and IgG1 titers were higher than those of IgG2a.
Keywords
Human papillomavirus; virus-like particles; splenocytes proliferation; cytokines; Th1/Th2 response;
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Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
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