Browse > Article

Functional Dissection of Sigma-like Domain in Antibiotic Regulatory Gene, afsR2 in Streptomyces lividans  

Kim Chang-Young (School of Chemical Engineering and Biotechnology, Department of Biological Engineering, Inha University)
Park Hyun-Joo (School of Chemical Engineering and Biotechnology, Department of Biological Engineering, Inha University)
Kim Eung-Soo (School of Chemical Engineering and Biotechnology, Department of Biological Engineering, Inha University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.9, 2006 , pp. 1477-1480 More about this Journal
Abstract
The 63-amino-acid-encoding afsR2 is a global antibiotics-stimulating regulatory gene identified from the chromosome of Streptomyces lividans. To dissect a putative functional domain in afsR2, several afsR2-derivative deletion constructs were generated and screened for the loss of actinorhodin-stimulating capability. The afsR2-derivative construct missing a 50-bp C-terminal region significantly lost its actinorhodin-stimulating capability in S. lividans. In addition, site-directed mutagenesis on amino acid positions of #57-#61 in a 50-bp C-terminal region, some of which are conserved among known Sigma 70 family proteins, significantly changed the AfsR2's activity. These results imply that the C-terminal region of AfsR2 is functionally important for antibiotics-stimulating capability and the regulatory mechanism might be somehow related to the sigma-like domain present in the C-terminal of AfsR2.
Keywords
afsR2; Streptomyces; antibiotic regulation; sigma factor;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
연도 인용수 순위
1 Matsumoto, A., S. K. Hong, H. Ishizuka, S. Horinouchi, and T. Beppu. 1994. Phosphorylation of the AfsR protein involved in secondary metabolism in Streptomyces species by an eukaryotic-type protein kinase. Gene 146: 47-56   DOI   ScienceOn
2 Stein, D. and S. N. Cohen. 1989. A cloned regulatory gene of Streptomyces lividans can suppress the pigment deficiency phenotype of different development mutants. J. Bacteriol. 171:2258-2261   DOI
3 Zhao, X.-Q., K.-R. Kim, L.-W. Sang, S.-H. Kang, Y.-Y. Yang, and J.-W. Suh. 2005. Genetic organization of a 50-kb gene cluster isolated from Streptomyces kanamyceticus for kanamycin biosynthesis and characterization of kanamycin acetyltransferase. J. Microbiol. Biotechnol. 15: 346-353   과학기술학회마을
4 Champness, W. C. and K. F. Chater. 1994. Regulation and integration of antibiotic production and morphological differentiation in Streptomyces spp., pp. 61-93. In P. Piggot et al. (eds.). Regulation of Bacterial Differentiation. American Society for Microbiology, Washington D.C
5 Hara, O., S. Horinouchi, T. Uozumi, and T. Beppu. 1983. Genetic analysis of A-factor synthesis in Streptomyces coelicolor A3(2) and Streptomyces griseus. J. Gen. Microbiol. 129: 2939-2944
6 Horinouchi, S., O. Hara, and T. Beppu. 1983. Cloning of a pleiotropic gene that positively controls biosynthesis of A-factor, actinorhodin, and prodigiosin in Streptomyces coelicolor A3(2) and Streptomyces lividans. J. Bacteriol. 155: 1238-1248
7 Horinouchi, S., M. Kito, M. Nishiyama, K. Furuya, S. K. Hong, K. Miyake, and T. Beppu. 1992. Primary structure of AfsR, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2). Gene 95: 49-56   DOI   ScienceOn
8 Kim, E. S., H. J. Hong, C. Y. Choi, and S. N. Cohen. 2001. Modulation of actinorhodin biosynthesis in Streptomyces lividans by glucose repression of afsR2 gene transcription. J. Bacteriol. 183: 2198-2203   DOI   ScienceOn
9 Horinouchi, S. 2003. AfsR as an integrator of signals that are sensed by multiple serine/threonine kinases in Streptomyces coelicolor A3(2). J. Ind. Microbiol. Biotechnol. 30: 462-467   DOI
10 Kim, C. Y., H. J. Park, Y. J. Yoon, H. Y. Kang, and E. S. Kim. 2004. Stimulation of actinorhodin production by Streptomyces lividans with a chromosomally-integrated antibiotic regulatory gene afsR2. J. Microbiol. Biotechnol. 14: 1089-1092
11 Chater, K. F. 1990. Multilevel regulation of Streptomyces differentiation. Trends Genet. 5: 372-377   DOI   ScienceOn
12 Matsumoto, A., H. Ishizukz, T. Beppu, and S. Horinouchi. 1995. Involvement of a small ORF downstream of the afsR gene in the regulation of secondary metabolism in Streptomyces coelicolor A3(2). Actinomycetologica 9: 37-43   DOI   ScienceOn
13 Floriano, B. and M. J. Bibb. 1996. afsR is a pleiotropic but conditionally required regulatory gene for antibiotic production in Streptomyces coelicolor A3(2). Mol. Microbiol. 21: 385-396   DOI   ScienceOn
14 Hopwood, D. A. 1987. Towards an understanding of gene switching in Streptomyces: The basis of sporulation and antibiotic production. Proc. R. Soc. Lond. Series B 235: 2257-2269
15 Hopwood, D. A., M. J. Bibb, K. J. Chater, T. Kieser, C. J. Bruton, H. M. Kieser, D. J. Lydiate, W. P. Smith, J. M. Ward, and H. Schrempf. 1985. Genetic Manipulation of Streptomyces -- A Laboratory Manual. The John Innes Foundation, Norwich, England
16 Strauch, E., E. Takano, H. A. Baylis, and M. J. Bibb. 1991. The stringent response in Streptomyces coelicolor A3(2). Mol. Microbiol. 5: 289-298   DOI   ScienceOn
17 Horinouchi, S., F. Malpartida, D. A. Hopwood, and T. Beppu. 1989. afsB stimulates transcription of the actinorhodin biosynthetic pathway in Streptomyces coelicolor A3(2) and Streptomyces lividans. Mol. Gen. Genet. 215: 355-357   DOI
18 Park, N. S., J. S. Myeong, H.-J. Park, K. Han, S.-N. Kim, and E.-S. Kim. 2005. Characterization and culture optimization of regiospecific cyclosporin hydroxylation in rare actinomycetes species. J. Microbiol. Biotechnol. 15: 188-191   과학기술학회마을
19 Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning --A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y
20 Vogtli, M., P. C. Chang, and S. N. Cohen. 1994. afsR2: A previously undeleted gene encoding a 63-amino-acid protein that stimulates antibiotic production in Streptomyces lividans. Mol. Microbiol. 14: 643-653   DOI   ScienceOn
21 Hong, S. K., M. Kito, T. Beppu, and S. Horinouchi. 1991. Phosphorylation of the AfsR product, a global regulatory protein for secondary-metabolite formation in Streptomyces coelicolor A3(2). J. Bacteriol. 173: 2311 -2318   DOI
22 Park, H.-S., S.-H. Kang, H.-J. Park, and E.-S. Kim. 2005. Doxorubicin productivity improvement by the recombinant Streptomyces peucetius with high-copy regulatory genes cultured in the optimized media composition. J. Microbiol. Biotechnol. 15: 66-71   과학기술학회마을
23 Champness, W. C, P. Riggle, T. Adamidis, B. Kenney, and D. Aceti. 1993. Genetic elements involved in global antibiotic regulation in Streptomyces coelicolor, pp. 227-233. In R. Baltz et al. (eds.), Industrial Microorganisms: Basic and Applied Molecular Genetics. American Society for Microbiology, Washington D.C