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Sequence-Based Screening for a Putative ${\gamma}$-Butyrobetaine Hydroxylase Gene from Neurospora crassa  

Hur Min-Sang (Division of Animal Science and Biotechnology, Sangji University)
Cho Jae-Yong (Division of Animal Science and Biotechnology, Sangji University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.9, 2006 , pp. 1468-1471 More about this Journal
Abstract
The last step in L-carnitine biosynthesis in eukaryotic organisms is mediated by ${\gamma}$-butyrobetaine hydroxylase (EC1.14.11.1), a dioxygenase that converts ${\gamma}$-butyrobetaine to L-carnitine. This enzyme was previously identified from rat liver and humans, and the peptide sequence of human ${\gamma}$-butyrobetaine hydroxylase was used to search the Neurospora crassa genome database, which led to an identification of an open reading frame (ORF) consisting of 1,407 bp encoding a polypeptide of 468 amino acids. When this protein was expressed in Saccharomyces cerevisiae, the crude cell-free extract exhibited ${\gamma}$-butyrobetaine hydroxylase activity.
Keywords
L-Carnitine; ${\gamma}$-butyrobetaine hydroxylase; cDNA library; Saccharomyces cerevisiae; Neurospora crassa;
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