Browse > Article

Screening of Yeast Diauxic Promoters for Production of Foreign Proteins  

Kim Jin-Ju (Department of Microbiology and Research Institute of Life Sciences, GyeongSang National University)
Kim Sang-Woo (Department of Microbiology and Research Institute of Life Sciences, GyeongSang National University)
Jeon Che-Ok (Environmental Biotechnology National Core Research Center, GyeongSang National University)
Yun Ji-Yun (Department of Microbiology and Research Institute of Life Sciences, GyeongSang National University)
Lee Hyun-Sook (Department of Microbiology and Research Institute of Life Sciences, GyeongSang National University)
Ro Hyeon-Su (Department of Microbiology and Research Institute of Life Sciences, GyeongSang National University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.9, 2006 , pp. 1459-1463 More about this Journal
Abstract
This study explored yeast diauxic promoters using a green fluorescent protein (GFP) reporter to screen growth phase-controlled promoters applicable for foreign protein production. Twenty-five diauxic promoters were inserted into a yeast 2-micron vector in front of the reporter GFP gene. The expressed GFP signal intensity measurements showed that 23 out of the 25 promoters produced a significant fluorescent signal when the cells were in the diauxic growth phase. Among the two strongest promoters pYDL204W and pYLR258W, the former remained constantly active after its activation at the diauxic shift, whereas the latter was only transiently activated right after the deprivation of the medium glucose.
Keywords
Diauxic promoter; glucose; yeast; GFP reporter; DNA microarray;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
연도 인용수 순위
1 Derisi, J. L., V. R. Iyer, and P. O. Brown. 1997. Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 278: 680-686   DOI   ScienceOn
2 Gietz, D., A. St. Jean, R. A. Woods, and R. H. Schiestl. 1992. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acid Res. 20: 1425   DOI   ScienceOn
3 Longtine, M. S., A. McKenzie, D. J. Demarini, N. G. Shah, A. Wach, A. Brachat, P. Philippsen, and J. R. Pringle. 1998. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14: 953-961   DOI   ScienceOn
4 Nishzawa, M., Y. Katou, K. Shirahige, and A. Toh-e. 2004. Yeast Pho85 kinase is required for proper gene expression during the diauxic shift. Yeast 21: 903-918   DOI   ScienceOn
5 Ro, H. S., M. S. Lee, M. S. Hahm, H. S. Bae, and B. H. Chung. 2005. Production of active carboxypeptidase Y of Saccharomyces cerevisiae secreted from methylotrophic yeast Pichiapastoris. J. Microbiol. Biotechnol. 15: 202-205   과학기술학회마을
6 Lee, J. S., J. Yu, H. J. Shin, Y S. Kim, J. K. Ahn, C. K. Lee, H. Poo, and C. J. Kim. 2005. Expression of hepatitis C virus structural proteins in Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 15: 767-771   과학기술학회마을
7 Dickinson, J. R. 1999. Carbon metabolism. In Dickinson, J. R. and M. Schweizer (eds.), The Metabolism and Molecular Physiology of Saccharomyces cerevisiae. Taylor & Francis, Philadelphia, PA
8 Chen, G, T. G. Gharib, C. C. Huang, J. M. Taylor, D. E. Misek, S. L. Kardia, T. J. Giordano, M. D. Iannettoni, M. B. Orringer, S. M. Hanash, and D. G. Beer. 2002. Discordant protein and mRNA expression in lung adenocarcinomas. Mol. Cell. Proteomics 1: 304-313   DOI
9 Enjalbert, B., J. L. Parrou, M. A. Teste, and J. Francois. 2004. Combinatorial control by the protein kinases PKA, PH085 and SNF1 of transcriptional induction of the Saccharomyces cerevisiae GSY2 gene at the diauxic shift. Mol. Genet. Genomics 271: 697-708
10 Greenbaum, D., C. Colangelo, K. Williams, and M. Gerstein. 2003. Comparing protein abundance and mRNA expression levels on a genomic scale. Genome Biol. 4:117   DOI
11 Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-429   DOI
12 Kim, H. E., R. Qin, and K. S. Chae. 2005. Increased production of exoinulinase in Saccharomyces cerevisiae by expressing the Kluyveromyces marxianus INU1 gene under the control of the INU1 promoter. J. Microbiol. Biotechnol. 15: 447-450   과학기술학회마을