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Proteomic Analysis of Resting and Activated Human $CD8^+$ T Cells  

Koo Jung-Hui (Department of Biochemistry, Protein Network Research Center, Yonsei University)
Chae Wook-Jun (Department of Biotechnology, Yonsei University)
Choi Je-Min (Department of Biochemistry, Yonsei University)
Nam Hyung-Wook (Department of Biochemistry, Protein Network Research Center, Yonsei University)
Morio Tomohiro (Department of Pediatrics, Tokyo Medical and Dental University, School of Medicine)
Kim Yu-Sam (Department of Biochemistry, Protein Network Research Center, Yonsei University)
Jang Yang-Soo (Cardiovascular Center, Yonsei University)
Choi Kwan-Yong (Department of Life Science, Pohang University of Science and Technology)
Yang Jung-Jin (School of Computer Science and Information Engineering, The Catholic University of Korea)
Lee Sang-Kyou (Department of Biotechnology, Yonsei University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.6, 2006 , pp. 911-920 More about this Journal
Abstract
[ $CD8^+$ ] T Iymphocytes with the cytotoxic activity and capability to release various cytokines are the major players in immune responses against viral infection and cancer. To identify the proteins specific to resting or activated human CD8$^+$ T cells, human CD8$^+$ T cells were activated with anti-CD3+anti-CD28 mAb in the presence of IL-2. The solubilized proteins from resting and activated human CD8$^+$ T cells were separated by high-resolution two-dimensional polyacrylamide gel electrophoresis, and their proteomes were analyzed. Proteomic analysis of resting and activated T cells resulted in identification of 35 proteins with the altered expression. Mass spectrometry coupled with Profound and SWISS-PROT database analysis revealed that these identified proteins are to be functionally associated with cell proliferation, metabolic pathways, antigen presentation, and intracellular signal transduction pathways. We also identified six unknown proteins predicted from genomic DNA sequences specific to resting or activated CD8$^+$ T cells. Protein network studies and functional characterization of these novel proteins may provide new insight into the signaling transduction pathway of CD8$^+$ T cell activation.
Keywords
Human CD8$^+$ T Iymphocytes; two-dimensional electrophoresis (2-DE); matrix-assisted laser desorption - time of flight mass spectrometry (MALDI-TOF MS); T-cell activation;
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