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Identification and Characterization of the wbpO Gene Essential for Lipopolysaccharide Synthesis in Vibrio vulnificus  

Park Na-Young (Department of Food Science and Technology, School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University)
Lee Jeong-Hyun (Department of Food Science and Technology, School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University)
Lee Byung-Cheol (School of Life Sciences and Biotechnology, Korea University)
Kim Tae-Sung (School of Life Sciences and Biotechnology, Korea University)
Choi Sang-Ho (Department of Food Science and Technology, School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.5, 2006 , pp. 808-816 More about this Journal
Abstract
A wbpO gene encoding a putative UDP-N-acetylo-galactosamine dehydrogenase was identified and cloned from Vibrio vulnificus. LPS production was altered by disruption of the wbpO gene through allelic exchanges. The function of the wbpO gene in virulence assessed in vitro and in mice revealed that WbpO is important in both the pathogenesis of V. vulnificus and the biosynthesis of LPS.
Keywords
V. vulnificus wbpO; LPS; pathogenesis;
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Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 9  (Related Records In Web of Science)
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1 De Lorenzo, V., M. Herrero, U. Jakubzik, and K. N. Timmis. 1990. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J. Bacteriol. 172: 6568-6572   DOI
2 Donnenberg, M. S. and J. B. Kaper. 1991. Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect. Immun. 59: 4310-4317
3 Espat, N. J., T. Auffenberg, A. Abouhamze, J. Baumhofer, L. L. Moldawer, and R. J. Howard. 1996. A role for tumor necrosis factor-alpha in the increased mortality associated with Vibrio vulnificus infection in the presence of hepatic dysfunction. Ann. Surg. 223: 428-433   DOI
4 Falkow, S. 1988. Molecular Koch's postulates applied to microbial pathogenicity. Rev. Infect. Dis. 10: S274-S276   DOI
5 Gulig, P. A., K. L. Bourdage, and A. M. Starks. 2005. Molecular pathogenesis of Vibrio vulnificus. J. Microbiol. 43: 118-131   과학기술학회마을
6 Hitchcock, P. J. and T. M. Brown. 1983. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154: 269-277
7 Ju, H. M., I. G. Hwang, G. J. Woo, T. S. Kim, and S. H. Choi. 2005. Identification of the Vibrio vulnificus fexA gene and evaluation of its influence on virulence. J. Microbiol. Biotechnol. 15: 1337-1345   과학기술학회마을
8 Keen, N. T., S. Tamaki, D. Kobayashi, and D. Trollinger. 1988. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene 70: 191-197   DOI   ScienceOn
9 Lee, B. C., S. H. Choi, and T. S. Kim. 2004. Application of sulforhodamine B assay for determining cytotoxicity of Vibrio vulnificus against human intestinal cells. J. Microbiol. Biotechnol. 14: 340-355
10 Lim, M. S., M. H. Lee, J. H. Lee, H. M. Ju, N. Y. Park, H. S. Jeong, J. E. Rhee, and S. H. Choi. 2005. Identification and characterization of the Vibrio vulnificus malPQ operon. J. Microbiol. Biotechnol. 15: 616-625   과학기술학회마을
11 McPherson, V. L., J. A. Watts, L. M. Simpson, and J. D. Oliver. 1991. Physiological effects of the lipopolysaccharide of Vibrio vulnificus on mice and rats. Microbios 67: 272-273
12 Rocchetta, H. L., L. L. Burrows, and J. S. Lam. 1999. Genetics of O-antigen biosynthesis in Pseudomonas aeruginosa. Microbiol. Mol. Biol. Rev. 63: 523-553
13 Reed, L. J. and H. Muench. 1938. A simple method of estimating fifty percent endpoints. Am. J. Hyg. 27: 439-497
14 Belanger, M., L. L. Burrows, and J. S. Lam. 1999. Functional analysis of genes responsible for the synthesis of the B-band O antigen of Pseudomonas aeruginosa serotype O6 lipopolysaccharide. Microbiology 145: 3505-3521   DOI
15 Kim, H. J., J. H. Lee, J. E. Rhee, H. S. Jeong, H. K. Choi, H. J. Chung, S. Ryu, and S. H. Choi. 2002. Identification and functional analysis of the putAP genes encoding Vibrio vulnificus proline dehydrogenase and proline permease. J. Microbiol. Biotechnol. 12: 318-326
16 Staskawicz, B., D. Dahlbeck, N. Keen, and C. Napoli. 1987. Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea. J. Bacteriol. 169: 5789-5794   DOI
17 Strom, M. and R. N. Paranjpye. 2000. Epidemiology and pathogenesis of Vibrio vulnificus. Microbes Infect. 2: 177-188   DOI   ScienceOn
18 Litwin, C. M., T. W. Rayback, and J. Skinner. 1996. Role of catechol siderophore synthesis in Vibrio vulnificus virulence. Infect. Immun. 64: 2834-2838
19 Miller, V. L. and J. J. Mekalanos. 1988. A novel suicide vector and its use in construction of insertion mutations: Osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170: 2575-2583   DOI
20 Oka, A., H. Sugisaki, and M. Takanami. 1981. Nucleotide sequence of the kanamycin resistance transposon Tn903. J. Mol. Biol. 147: 217-226   DOI
21 Xu, D. Q., J. O. Cisar, N. Ambulos, Jr., D. H. Burr, and D. J. Kopecko. 2002. Molecular cloning and characterization of genes for Shigella sonnei form I O polysaccharide: Proposed biosynthetic pathway and stable expression in a live Salmonella vaccine vector. Infect. Immun. 70: 4414-4423   DOI
22 Zhao, X., C. Creuzenet, M. Belanger, E. Egbosimba, J. Li, and J. S. Lam. 2000. WbpO, a UDP-N-acetyl-$_D$galactosamine dehydrogenase from Pseudomonas aeruginosa serotype O6. J. Biol. Chem. 275: 33252-33259   DOI   ScienceOn
23 Rhee, J. E., J. H. Rhee, P. Y. Ryu, and S. H. Choi. 2002. Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress. FEMS Microbiol. Lett. 208: 245-251   DOI
24 Rhee, J. E., H. M. Ju, U. Park, B. C. Park, and S. H. Choi. 2004. Identification of the Vibrio vulnificus cadC and evaluation of its role in acid tolerance. J. Microbiol. Biotechnol. 14: 1093-1098
25 Slauch, J. M., M. J. Mahan, P. Michetti, M. R. Neutra, and J. J. Mekalanos. 1995. Acetylation (O-factor 5) affects the structural and immunological properties of Salmonella typhimurium lipopolysaccharide O antigen. Infect. Immun. 63: 437-441
26 Brown, I. I. and C. C. Hase. 2001. Flagellum-independent surface migration of Vibrio cholerae and Escherichia coli. J. Bacteriol. 183: 3784-3790   DOI   ScienceOn
27 Jeong, K. C., H. S. Jeong, J. H. Rhee, S. E. Lee, S. S. Chung, A. M. Starks, G. M. Escudero, P. A. Gulig, and S. H. Choi. 2000. Construction and phenotypic evaluation of Vibrio vulnificus vvpE mutant for elastolytic protease. Infect. Immun. 68: 5096-5106   DOI
28 Linkous, D. A. and J. D. Oliver. 1999. Pathogenesis of Vibrio vulnificus. FEMS Microbiol. Lett. 174: 207-214   DOI
29 Powell, J. L., A. C. Wright, S. S. Wasserman, D. M. Hone, and J. G. Morris, Jr. 1997. Release of tumor necrosis factor alpha in response to Vibrio vulnificus capsular polysaccharide in in vivo and in vitro models. Infect. Immun. 65: 3713-3718
30 Wright, A. C. and J. G. Morris, Jr. 1991. The extracellular cytolysin of Vibrio vulnificus: Inactivation and relationship to virulence in mice. Infect. Immun. 59: 192-197
31 Lee, J. H., N. Y. Park, S. J. Park, and S. H. Choi. 2003. Identification and characterization of the Vibrio vulnificus phosphomannomutase gene. J. Microbiol. Biotechnol. 13: 149-154
32 Shepherd, J. G., L. Wang, and P. R. Reeves. 2000. Comparison of O-antigen gene clusters of Escherichia coli (Shigella) Sonnei and Plesiomonas shigelloides O17: Sonnei gained its current plasmid-borne O-antigen genes from P. shigelloides in a recent event. Infect. Immun. 68: 6056-6061   DOI
33 Creuzenet, C., M. Belanger, W. W. Wakarchuk, and J. S. Lam. 2000. Expression, purification, and biochemical characterization of WbpP, a new UDP-GlcNAc C4 epimerase from Pseudomonas aeruginosa serotype O6. J. Biol. Chem. 275: 19060-19067   DOI   ScienceOn
34 Gulig, P. A. 1993. Use of isogenic mutants to study bacterial virulence factors. J. Microbiol. Methods 18: 275-287   DOI   ScienceOn
35 Makin, S. A. and T. J. Beveridge. 1996. The influence of A-band and B-band lipopolysaccharide on the surface characteristics and adhesion of Pseudomonas aeruginosa to surfaces. Microbiology 142: 299-307   DOI   ScienceOn
36 Proctor, R. A., L. C. Denlinger, and P. J. Bertics. 1995. Lipopolysaccharide and bacterial virulence, pp. 173-194. In J. A. Roth, C. A. Bolin, K. A. Brogden, F. C. Minnon, and M. J. Wannemuehler (eds.), Virulence Mechanisms of Bacterial Pathogens, 2nd Ed. ASM Press, Washington, D.C., U.S.A