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Monitoring of Aflatoxin $B_1$ in Livestock Feeds Using ELISA and HPLC  

Han Eun-Mee (Division of Bioscience & Biotechnology, Konkuk University)
Park Hee-Ra (National Institute of Toxicological Research)
Hu Soo-Jung (National Institute of Toxicological Research)
Kwon Ki-Sung (National Institute of Toxicological Research)
Lee Hyo-Min (National Institute of Toxicological Research)
Ha Mi-Sun (Division of Bioscience & Biotechnology, Konkuk University)
Kim Kyung-Mi (Division of Bioscience & Biotechnology, Konkuk University)
Ko Eun-Jung (Division of Bioscience & Biotechnology, Konkuk University)
Ha Sang-Do (Department of Food Science and Technology, Chung-Ang University)
Chun Hyang-Sook (Korea Food Research Institute)
Chung Duck-Hwa (Department of Food Science & Technology, Gyeongsang National University)
Bae Dong-Ho (Division of Bioscience & Biotechnology, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.4, 2006 , pp. 643-646 More about this Journal
Abstract
Because of potential health hazards of aflatoxins for humans, the present study was conducted to monitor aflatoxin $B_1\;(AFB_1)$ in livestock feeds. A total of 249 samples of feeds collected in Korea were analyzed by DC-ELISA for qualitative analysis of $AFB_1$. Then, 27 samples that were verified to contain $AFB_1$ by DC-ELISA were quantitated by HPLC/FLD. HPLC/FLD analysis revealed that only one sample collected from a farm contained 11 ppb of $AFB_1$, whereas the other samples collected from feed companies did not contain $AFB_1$. The presence of $AFB_1$ was further confirmed by LC/MS analysis. TLC analysis indicated that the result of the DC-ELISA was most likely due to possible contamination of other mycotoxins rather than $AFB_1$. In conclusion, HPLC/FLD analysis following DC-ELISA is necessary for rapid and accurate detection of $AFB_1$.
Keywords
Aflatoxin $B_1$; feed; ELISA; HPLC/FLD; LC/MS;
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Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
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