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Effect of Culture Conditions on Astaxanthin Formation in Red Yeast Xanthophyllomyces dendrorhous Mutant JH1  

Kim Jeong-Hwan (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University)
Choi Seok-Keun (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University)
Park Young-Sam (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University)
Yun Cheol-Won (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University)
Cho Won-Dai (Agricultural Cooperative College)
Chee Kew-Mahn (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University)
Chang Hyo-Ihl (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.3, 2006 , pp. 438-442 More about this Journal
Abstract
The formation of astaxanthin by Xanthophyllomyces dendrorhous mutant JH1 depends on the culture conditions. Therefore, the effects of inoculation rate (1-5%, v/v) and medium compositions (various carbon and nitrogen sources) on cell growth and astaxanthin formation in X. dendrorhous mutant JH1 were investigated. Inoculation at 3% (v/v) was optimal for cell growth and astaxanthin formation. The most effective carbon source for cell growth and astaxanthin formation was glucose, and the best nitrogen source was yeast extract. The 3% (w/v) glucose and 0.2% (w/v) yeast extract showed the best effect on cell growth and astaxanthin formation, compared with others tested. The 3% glucose, 0.2% yeast extract, $0.15%\;KH_{2}PO_{4}$, $0.05%\;MgSO_4$, $0.01%\;MnSO_4$, and $0.01%\;CaCl_2$ were selected for cell growth and astaxanthin formation. Under the conditions selected, the maximum concentrations of cell and astaxanthin obtained after 168 h of cultivation were 5.43 g/l and 28.20 mg/l, respectively.
Keywords
Astaxanthin; carbon source; culture conditions; nitrogen source; Xanthophyllomyces dendrorhous;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 13  (Related Records In Web of Science)
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1 Jyonouchi, H., S. Sun, K. Lijima, and M. D. Gross. 2000. Antitumor activity of astaxanthin and its mode of action. Nutr. Cancer 36: 59-65   DOI   ScienceOn
2 Kim, J. H., S. K. Choi, W. J. Lim, and H. I. Chang. 2004. Protective effect of astaxanthin produced by Xanthophyllomyces dendrorhous mutant on indomethacininduced gastric mucosal injury in Rats. J. Microbiol. Biotechnol. 14: 996-1003   과학기술학회마을
3 Kim, J. H., S. W. Kim, C. W. Yun, and H. I. Chang. 2005. Therapeutic effect of astaxanthin isolated from Xanthophyllomyces dendrorhous mutant against naproxeninduced gastric antral ulceration in Rats. J. Microbiol. Biotechnol. 15: 633-639   과학기술학회마을
4 Krinsky, N. I. 1989. Antioxidant function of carotenoids. Free Radic. Biol. Med. 7: 617-635   DOI   ScienceOn
5 Naguib, Y. M. 2000. Antioxidant activities of astaxanthin and related carotenoids. J. Agric. Food Chem. 48: 1150- 1154   DOI   ScienceOn
6 Simpson, K. I., T. Katayana, and C. O. Chichester. 1981. Carotenoids from microorganisms. In Bauernfeld, J. C. (ed.), Carotenoids as Colorants and Vitamin A Precursors. Academic Press, London, U.K
7 Park, E. K. and C. G. Lee. 2001. Astaxanthin production by Haematococcus pluvialis under various light intensities and wavelengths. J. Microbiol. Biotechnol. 11: 1024-1030
8 Sedmak, J. J., D. K. Weerasinghe, and S. O. Jolly. 1990. Extraction and quantitation of astaxanthin from Phaffia rhodozyma. Biotech. Tech. 4: 107-112   DOI
9 Bubrick, P. 1991. Production of astaxanthin from Haematococcus. Bioresource Technol. 38: 237-239   DOI   ScienceOn
10 Kim, J. H., Y. S. Kim, G. G. Song, J. J. Park, and H. I. Chang. 2005. Protective effect of astaxanthin on naproxen-induced gastric antral ulceration in Rats. Eur. J. Pharmacol. 514: 53- 59   DOI   ScienceOn
11 Johnson, E. A. and M. J. Lewis. 1979. Astaxanthin formation by the yeast Phaffia rhodozyma. J. Gen. Microbiol. 115: 173- 183   DOI
12 Johnson, E. A. and W. A. Schroeder. 1995. Microbial carotenoids. Adv. Biochem. Eng. 53: 119-178
13 Bertram, J. S. 1993. Cancer prevention of carotenoids: Mechanistic studies in cultured cells. Ann. NY Acad Sci. 691: 177-191   DOI
14 Bendich, A. and J. A. Olson. 1989. Biological actions of carotenoids. FASEB J. 3: 1927-1932   DOI
15 Johnson, E. A., D. E. Conklin, and M. J. Lewis. 1977. The yeast Phaffia rhodozyma as a dietary pigment source for salmonoids and crustaceans. J. Fish Res. Bd. Canada 34: 2417-2421   DOI
16 Mortensen, A., L. H. Skibsted, and T. Truscott. 2001. The interaction of dietary carotenoids with radical species. Arch. Biochem. Biophys. 385: 13-19   DOI   ScienceOn
17 Goodwin, T. W. 1980. The Biochemistry of the Carotenoids. Second Edition. Chapman & Hall, London, U.K
18 Chew, B. P. 1993. Role of carotenoids in the immune response. J. Dairy Sci. 76: 2804-2811   DOI   ScienceOn
19 Johnson, E. A. and G. H. An. 1991. Astaxanthin from microbial sources. Crit. Rev. Biotechnol. 11: 297-326   DOI
20 Kim, J. H., C. W. Kim, and H. I. Chang. 2004. Screening and characterization of red yeast Xanthophyllomyces dendrorhous mutants. J. Microbiol. Biotechnol. 14: 570-575