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Selection and Characterization of Peptides Specifically Binding to $TiO_2$ Nanoparticles  

Seo Min-Hee (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studie)
Lee Jong-Ho (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studie)
Kim Min-Soo (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studie)
Chae Hee-K. (Department of Chemistry Education, Seoul National University)
Myung Hee-Joon (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studie)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.2, 2006 , pp. 303-307 More about this Journal
Abstract
We have screened phage display peptide libraries to select for peptides binding to various sized $TiO_2$ nanoparticles. Phage libraries displaying random 7mer, 12mer, and C-7-Cmer peptides were used for screening. The size of target $TiO_2$ particles used were 7 nm, 15 nm, and 25 nm in diameter. We could select peptides binding each nanoparticles from all 3 libraries. Their binding was confirmed by transmission electron microscopy (TEM). Each peptide investigated was also shown to bind the other sized particles, meaning that the binding was specific for the nature of the particle rather than for the size of it. One of the 7mer peptides (PEP9, SVSPISH) was chosen for further analysis. The binding was shown to be in a dose-dependent manner, suggesting a specific interaction.
Keywords
Nanoparticle; phage display; peptide;
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Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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