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Enzymatic Synthesis of Ascorbic Acid Fructoside by Transfructosylation Using Levan Fructotransferase  

LEE CHOONG YEUL (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
KIM KI HO (R&D Center, Bioland Ltd.,)
HUR SUN YEON (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
HEO JOO-HYUNG (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
CHOI MIN HO (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
RHEE SANG KI (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
KIM CHUL HO (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.1, 2006 , pp. 64-67 More about this Journal
Abstract
To enhance the stability of ascorbic acid, the glycosylation of ascorbic acid was studied using the transfructosylation activity of levan fructotransferase. When levan was used as glycosyl donor, a novel fructoside (ascorbic acid 2-ffuctoside) was formed by the transfructosylation activity of the levan fructotransferase. The production of ascorbic acid 2-fructoside was highly affected by the concentration of the fructosyl acceptor (ascorbic acid). When $35\%$ of ascorbic acid and $2\%$ of levan were incubated with LFTase of 0.5 unit/glevan at $37^{\circ}C$ for 85 h, a maximum 52 g/l of AA-2F was produced.
Keywords
Ascorbic acid 2-fructoside; fructoside; glycoside; glycosylation; levan fructotransferase;
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Times Cited By Web Of Science : 7  (Related Records In Web of Science)
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