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Inhibitory Effect of Ponciretin on Helicobacter pylori VacA Toxin-induced Vacuolation in HeLa Cells  

KIM JONG-MI (College of Pharmacy, Kyung Hee University)
SHIN JI-EUN (Department of Food and Nutrition, Kyung Hee University)
BAE EUN-AH (Department of Food and Nutrition, Kyung Hee University)
HAN MYUNG JOO (Department of Food and Nutrition, Kyung Hee University)
KIM DONG-HYUN (College of Pharmacy, Kyung Hee University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.1, 2006 , pp. 46-51 More about this Journal
Abstract
The inhibitory effects of flavanone derivatives on Helicobacter pylori (HP) growth, infection and VacA toxininduced vacuolation were investigated. Among flavanones tested, ponciretin potently inhibited the growth of HP with a MIC value of 0.01 mg/ml and VacA toxin-induced vacuolation in HeLa cells with $IC_{50}$ value of 0.078 mM. However, other flavanones inhibited neither HP growth nor VacA toxininduced vacuolation. All flavanones tested did not inhibit HP infection to KATO III cells. Ponciretin also inhibited activation of procaspase-3 to caspase-3 in HeLa cell induced by HP VacA toxin, but did not affect Bax and Bcl-2 protein levels. These findings indicate that ponciretin inhibits growth as well as vacuolation by HP VacA toxin, which induces cell death via proteolytic activation of a cascade of caspases.
Keywords
Helicobacter pylori; ponciretin; VacA toxin; vacuolation; caspase-3;
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Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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1 Bae, E. A., M. J. Han, N. J. Kim, and D.-H. Kim. 1998. Anti- Helicobacter pylori activity of herbal medicines. Biol. Pharm. Bull. 21: 990-992   DOI
2 Bae, E. A., M. J. Han, and D.-H. Kim. 1999. In vitro anti- Helicobacter pylori activity of some flavonoids and their metabolites. Planta Med. 65: 442-443   DOI   ScienceOn
3 Carmichael, J., W. G. DeGreff, A. F. Gazdar, J. D. Minna, and J. B. Mitchell. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chmosensitivity testing. Cancer Res. 47: 936-940
4 Fukai, T., A. Marumo, K. Kaitou, T. Kanda, and S. Terada, T. Nomura. 2002. Anti-Helicobacter pylori flavonoids from licorice extract. Life Sci. 71: 1449-1463   DOI   ScienceOn
5 Gebert, B., W. Fischer, and R. Haas. 2004. The Helicobacter pylori vacuolating cytotoxin: From cellular vacuolation to immunosuppressive activities. Rev. Physiol. Biochem. Pharmacol. 152: 205-220   DOI
6 Kamisago, S., M. Iwamori, T. Tai, K. Mitamura, Y. Yazaki, and K. Sugano. 1996. Role of sulfatides in adhesion of Helicobacter pylori to gastric cancer cells. Infect Immun. 64: 624-628
7 Nanjo, F., R. Goto, M. Seto, M. Suzuki, M. Sakai, and Y. Hara. 1995. Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical. Free Radical Biol. Med. 21: 895-902   DOI   ScienceOn
8 Warren, J. R. and B. J. Marshall. 1983. Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 1: 1273-1275
9 Kim, S. M., K. H. Lee, N. K. Lee, C. J. Kim, C. J. Kim, C. H. Kim, and H. D. Paik. 2004. Antagonic activity of polyfermenticin SCD against Helicobacter pylori KCTC 2948. J. Microbiol. Biotechnol. 14: 148-152
10 Maeda, S., H. Yoshida, Y. Mitsuno, Y. Hirata, K. Ogura, Y. Shiratori, and M. Omata. 2002. Analysis of apoptotic and antiapoptotic signaling pathways induced by Helicobacter pylori. Mol. Pathol. 55: 286-293   DOI
11 Woo, J. S., B. H. Ha, T. G. Kim, Y. Lim, and K. H. Kim. 2003. Inhibition of Helicobacter pylori adhesion by acidic polysaccharide isolated from Artemisia capillaries. J. Microbiol. Biotechnol. 13: 853-858   과학기술학회마을
12 de Bernard, M., B. Arico, E. Papini, R. Rizzoto, G. Grandi, R. Rappuoli, and C. Montecucco. 1997. Helicobacter pylori toxin VacA induces vacuole formation by acting in the cell cytosol. Mol. Microbiol. 26: 665-674   DOI   ScienceOn
13 Kuck, D., B. Kolmerer, C. Iking-Konert, P. H. Krammer, W. Stremmel, and J. Rudi. 2001. Vacuolating cytotoxin of Helicobacter pylori induces apoptosis in the human gastric epithelial cell line AGS. Infect. Immun. 69: 5080-5087   DOI   ScienceOn
14 Eaton, K. A., C. L. Brooks, D. R. Morgan, and S. Krakowka. 1991. Essential role of urease in pathogenesis of gastritis induced by Helicobacter pylori in gnotobiotic piglets. Infect. Immun. 59: 2470-2475
15 Bae, E. A., M. J. Han, and D. H. Kim. 2001. In vitro anti- Helicobacter pylori activity of irisolidone isolated from the flowers and rhizomes of Pueraria thunbergiana. Planta Med. 67: 161-163   DOI   ScienceOn
16 Gutmann, I. and H. U. Bergmeyer. 1974. Urea, pp. 1791-1794. In H. U. Mergmeyer (ed.), Methods of Enzymatic analysis, vol. 4, Academic press, Inc., New York, U.S.A
17 Cover, T. L. and M. J. Blaser. 1992. Purification and characterization of the vacuolating toxin from Helicobacter pylori. J. Biol. Chem. 67: 10570-10575
18 Boquet, P., V. Ricci, A. Galmiche, and N. C. Gauthier. 2004. Gastric cell apoptosis and H. pylori: Has the main function of VacA finally been identified? Trends Microbiol. 11: 410- 413   DOI   ScienceOn
19 Mabe, K., M. Yamada, I. Oguni, and T. Takahashi. 1999. In vitro and in vivo activities of tea catechins against Helicobacter pylori. Antimicrob. Agents Chemother. 43: 1788-1791
20 Chen, P. C., D. S. Wheeler, V. Malhotra, K. Odoms, A. G. Denenberg, and H. R. Wong. 2002. A green tea-derived polyphenol, epigallocatechin-3-gallate, inhibits IkappaB kinase activation and IL-8 gene expression in respiratory epithelium. Inflammation 26: 233-241   DOI   ScienceOn
21 Tsujii, M., N. Kawano, S. Tsujii, H. Fusamoto, T. Kamada, and N. Sato. 1992. Mechanism of gastric mucosal damage induced by ammonia. Gastroenterology 102: 1881-1888   DOI
22 Goodwin, C. S. 1993. Gastric cancer and Helicobacter pylori: The whispering killer? Lancet 342: 507-508   DOI   ScienceOn
23 Ki, M. R., S. K. Yun, K. M. Choi, and S. Y. Hwang. 2003. Potential and significance of ammonium production from Helicobacter pylori. J. Microbiol. Biotechnol. 13: 673-679
24 Jones, N. L., A. S. Day, H. A. Jennings, and P. M. Sherman. 1999. Helicobacter pylori induces gastric epithelial cell apoptosis in association with increased Fas receptor expression. Infect. Immun. 67: 4237-4242
25 Handa, O., Y. Naito, T. Takagi, T. Ishikawa, M. Ueda, N. Matsumoto, S. Kokura, H. Ichikawa, N. Yoshida, K. Shimoi, and T. Yoshikawa. 2002. Inhibitory effects of catechins on neutrophil-dependent gastric inflammation. Redox Report 7: 324-328   DOI   ScienceOn
26 Cover, T. L., W. Puryear, G. I. Perez-perez, and M. J. Blaser. 1991. Effect of urease on HeLa cell vacuolation induced by Helicobacter pylori cytotoxin. Infect. Immun. 59: 1264-1270
27 Kim, J. S., J. M. Kim, H. C. Jung, and I. S. Song. 2001. Caspase-3 activity and expression of Bcl-2 family in human neutrophils by Helicobacter pylori water-soluble proteins. Helicobacter 6: 207-215   DOI   ScienceOn
28 Kim, J.-M., J.-E. Shin, M. J. Han, S. H. Park, and D.-H. Kim. 2003. Inhibitory effect of ginseng saponins and polysaccharides on infection and vacuolation of Helicobacter pylori. J. Microbiol. Biotechnol. 13: 706-709
29 Leunk, R. D., P. T. Johnson, B. C. David, W. G. Kraft, and D. R. Morgan. 1988. Cytotoxic activity in broth-culture filtrates of Campylobacter pylori. J. Med. Microbiol. 26: 93-99   DOI