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Antibody Layer Fabrication for Protein Chip to Detect E. coli O157:H7, Using Microcontact Printing Technique  

KIM HUN-SOO (Department of Chemical and Biomolecular Engineering, and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Technology Planning Team, Corporate R&D Center, Samsung SDI Co. Ltd,)
BAE YOUNG-MIN (Department of Chemical and Biomolecular Engineering, and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
KIM YOUNG-KEE (Department of Chemical Engineering, Hankyong National University)
OH BYUNG-KEUN (Department of Chemical and Biomolecular Engineering, and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
CHOI JEONG-WOO (Department of Chemical and Biomolecular Engineering, and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.1, 2006 , pp. 141-144 More about this Journal
Abstract
An antibody layer was fabricated to detect Escherichia coli O157:H7. The micropattern of 16-mercaptohexadecanoic acid (16-MHDA) as alkylthiolate was formed on the gold surface by using the PDMS stamp with microcontact printing $({\mu}CP)$ techniques. In order to form antibody patterns on the template, protein G was chemically bound to the 16-MHDA patterns, and antibody was adsorbed on a self-assembled protein G layer. The formation of the 16-MHDA micropattern, self-assembled protein G layer and antibody pattern on Au substrate was confirmed by surface plasmon resonance (SPR) spectroscopy. Finally, the micropatterning method was applied to fabricate the antibody probe for detection of E. coli O157:H7, and monitoring of antigen by using this probe was successfully achieved.
Keywords
Antibody probe; protein G; mercaptohexadecanoic acid; microcontact printing; micropattern; E. coli O157:H7;
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1 Graber, D. J., T. J. Zieziuewicz, D. A. Lawrence, W. Shain, and J. N. Turner. 2003. Antigen binding specificity of antibodies patterned by microcontact printing. Langmuir 19: 5431-5434   DOI   ScienceOn
2 Kwak, S. K., G. S. Lee, D. J. Ahn, and J. W. Choi. 2004. Pattern formation of cytochrome c by microcontact printing and dip-pen nanolithography. Mater. Sci. Engineer. C 24: 151-155   DOI   ScienceOn
3 Libioulle, L., A. Bietsch, H. Schmid, B. Michel, and E. Delamarche. 1999. Contact-inking stamps for microcontact printing of alkanethiols on gold. Langmuir 15: 300-304   DOI   ScienceOn
4 Morhard, F., J. P. R. Dahint, and M. Grunze. 2000. Immobilization of antibodies in micropatterns for cell detection by optical diffraction. Sens. Actuators B 70: 232- 242   DOI   ScienceOn
5 Oh, B. K., Y. K. Kim, W. Lee, Y. M. Bae, W. H. Lee, and J. W. Choi. 2003. Immunosensor for detection of Legionella pneumophila using surface plasmon resonance. Biosens. Bioelectron. 18: 605-611   DOI   ScienceOn
6 Patel, N., M. C. Davies, M. Hartshorne, R. J. Heaton, C. J. Roberts, S. J. B. Tendler, and P. M. Williams. 1997. Immobilization of protein molecules onto homogeneous and mixed carboxylate-terminated self-assembled monolayer. Langmuir 13: 6485-6490   DOI   ScienceOn
7 Wang, J., Y. Bai, T. Li, and Z. Lu. 2003. DNA microarrays with unimolecular hairpin double-stranded DNA probes: fabrication and exploration of sequence-specific DNA/protein interactions. J. Biochem. Biophys. Methods 55: 215-232   DOI   ScienceOn
8 Renault, J. P., A. Bernard, D. Juncker, B. Michel, H. R. Bosshard, and E. Delamarche. 2002. Fabricating microarrays of functional proteins using affinity contact printing. Angew. Chem. Int. Ed. Engl. 41: 2320-2323   DOI   ScienceOn
9 Boyle, M. D. P. and K. J. Reis. 1987. Bacterial Fc receptors. Biotechnology 5: 697-703   DOI
10 Chen, S., L. Liu, J. Zhou, and S. Jiang. 2003. Controlling antibody orientation on charged self-assembled monolayers. Langmuir 19: 2859-2864   DOI   ScienceOn
11 Oh, B. K., Y. K. Kim, Y. M. Bae, W. H. Lee, and J. W. Choi. 2002. Detection of Escherichia coli O157:H7 using immunosensor based on surface plasmon resonance. J. Microbiol. Biotechnol. 12: 780-786
12 Kretschmann, E. 1971. Die bestimmung optischer konstanten von metallen durch anregung von oberflachenplasmaschwingungen. Z. Phys. 241: 313-324   DOI
13 Bernard, A., B. Michel, and E. Delamarche. 2001. Micromosaic immunoassay. Anal. Chem. 73: 8-12   DOI   ScienceOn
14 Xia, Y. and G. M. Whitesides. 1998. Soft lithography. Angew. Chem. Int. Ed. Engl. 37: 550-575   DOI   ScienceOn
15 Shin, D. S., K. N. Lee, K. H. Jang, J. K. Kim, W. J. Chung, Y. K. Kim, and Y. S. Lee. 2003. Protein patterning by maskless photolithography on hydrophilic polymer-grafted surface. Biosens. Bioelectr. 19: 485-494   DOI   ScienceOn