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Mediation of Rubradirin Resistance by ABC Transporters (RubT1) from Streptomyces achromogenes var. rubradiris NRRL3061  

Lamichhane, Janardan (Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, SunMoon University)
Oh, Tae-Jin (Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, SunMoon University)
Lee, Hei-Chan (Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, SunMoon University)
Liou, Kwang-Kyoung (Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, SunMoon University)
Kim, Chun-Gyu (Department of Pharmaceutical Engineering, Inje University)
Sohng, Jae-Kyung (Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, SunMoon University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.12, 2006 , pp. 1928-1934 More about this Journal
Abstract
The rubradirin biosynthetic gene cluster harbors 58 ORFs within a 105.6-kb sequence, which includes all of the genes responsible for the synthesis of rubradirin, as well as the primary genes relevant to regulatory, resistance, and transport functions. This gene cluster also harbors a resistance-mediating ABC transporter, RubT1, which is located at the most upstream position in the cluster. In the present study, RubT1 was expressed heterologously in E. coli, and the resistance affinity of RubT1 was determined by an antibacterial activity test, as well as by HPLC and ESI-MS analyses. Evidence clearly demonstrates that RubTl mediates rubradirin resistance as an ABC transporter.
Keywords
ABC transporter; membrane-spanning domain; resistance gene; rubradirin; S. achromogenes;
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Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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