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Screening and Purification of a Novel Transaminase Catalyzing the Transamination of Aryl ${\beta}-Amino$ Acid from Mesorhizobium sp. LUK  

Kim, Ju-Han (Institute for Molecular Biology and Genetics, and School of Chemical and Biological Engineering, Seoul National University)
Kyung, Do-Hyun (Institute for Molecular Biology and Genetics, and School of Chemical and Biological Engineering, Seoul National University)
Yun, Hyung-Don (Institute for Molecular Biology and Genetics, and School of Chemical and Biological Engineering, Seoul National University)
Cho, Byung-Kwan (Institute for Molecular Biology and Genetics, and School of Chemical and Biological Engineering, Seoul National University)
Kim, Byung-Gee (Institute for Molecular Biology and Genetics, and School of Chemical and Biological Engineering, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.11, 2006 , pp. 1832-1836 More about this Journal
Abstract
Mesorhizobium sp. LUK, which utilizes 3-amino-3-phenylpropionic acid as the sole source of nitrogen with high enantioselectivity (E(S)>100), was isolated using enrichment culture. The enzyme involved in the utilization of (S)-3-amino-3-phenylpropionic acid was confirmed to be a transaminase and was purified by 235-folds with a specific activity of 0.72 U/mg. The molecular weight of the purified protein was ca. 47 kDa and the active enzyme was determined as a dimer on gel filtration chromatography. The N-terminal sequence was obtained from the purified protein. Spontaneous decarboxylation of produced ${\beta}-keto$ acids was observed during the chiral resolution of 3-amino-3-phenylpropionic acid.
Keywords
Transaminase; ${\beta}-Amino$ acid; 3-amino-3-phenylpropionic acid; Mesorhizobium sp. LUK;
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