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Isolation and Characterization of Brain-Derived Neurotrophic Factor Gene from Flounder (Paralichthys olivaceus)  

LEE JAE HYUNG (Department of Microbiology, Pukyong National University)
CHOI TAE-JIN (Department of Microbiology, Pukyong National University)
NAM SOO WAN (Department of Biotechnology and Bioengineering, Dong-Eui University)
KIM YOUNG TAE (Department of Microbiology, Pukyong National University)
Publication Information
Journal of Microbiology and Biotechnology / v.15, no.4, 2005 , pp. 838-843 More about this Journal
Abstract
Brain-derived neurotrophic factor (BDNF) is a small secretory protein and a member of the nerve growth factor (NGF) gene family. We cloned the flounder BDNF gene from a flounder brain cDNA library. The nucleotide sequence of the cloned gene showed an open reading frame (ORF) consisting of 810 bp, corresponding to 269 amino acid residues. The tissue distribution of flounder BDNF was determined by reverse transcription-polymerase chain reaction (RT-PCR) in brain, embryo, and muscle tissues. To express fBDNF using a eukaryotic expression system, we constructed the vector mpCTV-BDNF containing the fBDNF gene and transformed this vector into Chlorella ellipsoidea. Stable integration of introduced DNA was confirmed by PCR analysis of genomic DNA, and mRNA expression in C. ellipsoidae was confirmed by RT-PCR analysis.
Keywords
Brain-derived neurotrophic factor; flounder; expression; RT-PCR;
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1 Lein, E. S., A. Hohn, and C. J. Shatz. 2000. Dynamic regulation of BDNF and NT-3 expression during visual system development. J. Comp. Neurol. 420: 1-18   DOI   ScienceOn
2 Maisonpierre, P. C., M. M. Le Beau, R. Espinosa 3rd, N. Y. Ip, L. Belluscio, S. M. de la Monte, S. Squinto, M. E. Furth, and O. D. Yancopoulos. 1991. Human and rat brain-derived neurotrophic factor and neurotrophin-3: Gene structures, distributions, and chromosomal localizations. Genomics 10: 558-568   DOI   PUBMED
3 Rodriguez- Tebar, A., P. L. Jeffrey, H. Thoene, and Y. A. Barde. 1989. The survival of chick retinal ganglion cells in response to brain-derived neurotrophic factor depends on their embryonic age. Dev. Biol. 136: 296-303   DOI   ScienceOn
4 Arab, S. F., K. Krohn, A. Lachmund, K. Unsicker, and C. Suter-Crazzolara. 1997. The gene encoding bovine brainderived neurotrophic factor (BDNF). Gene 185: 95-98   DOI   ScienceOn
5 Aronica, E., S. Leenstra, G H. Jansen, W. M. Cees, B. Yankaya, and D. Troost. 2001. Expression of brain-derived neurotrophic factor and tyrosine kinase B receptor proteins in glioneuronal tumors from patients with intractable epilepsy: Colocalization with N-methyl-D-aspartic acid receptor. Acta Neuropathol. 101: 383-392
6 Barbacid, M. 1994. J. Neurobiol. 25: 1386-1403   DOI   PUBMED   ScienceOn
7 Bonni, A., A. Brunet, A. E. West, S. R. Datta, M. A. Takasu, and M. E. Greenberg. 1999. Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms. Science 286: 1358-1362   DOI   PUBMED
8 Friehs, K. and K. K. Reardon. 1993. Parameters influencing the productivity of recombinant E. coli cultivations. Adv. Biochem. Eng. Biotechnol. 48: 53-77   DOI
9 Chan, J. R., J. M. Cosgaya, Y. J. Wu, and E. M. Shooter. 2001 Neurotrophins are key mediators of the myelination. program in the peripheral nervous system. Proc. Natl. Acad. Sci. USA 98: 14661-14668
10 Frade, J. M., P. Bovolenta, J. R. Martinez-Morales, A. Arribas, J. A. Barbas, and A. Rodriguez-Tebar. 1997. Control of early cell death by BDNF in the chick retina. Development 124: 3313-3320
11 Gottsch, W., L. D. Pozzo-Miller, A. Figurov, and B. Lu. 1998. Presynaptic modulation of synaptic transmission and plasticity by brain-derived neurotrophic factor in the developing hippocampus. J. Neurosci. 18: 6830-6839
12 Han, K., Y.- T. Jung, and S.- Y. Son. 2003. Phylogenetic analysis of phenanthrene-degrading Sphingomonas. J. Microbiol. Biotechnol. 13: 942-948
13 Johnson, J. E., Y. A. Barde, M. Schwab, and H. Thoene. 1986. Brain-derived neurotrophic factor supports the survival of cultured rat retinal ganglion cells. J. Neurosci. 6: 3031-3038   DOI
14 Jones, K. R. and L. F. Reichardt. 1990. Molecular cloning of a human gene that is a member of the nerve growth factor family. Proc. Natl. Acad. Sci. USA 87: 8060-8064
15 Kim, H.-S. and T. J. Crow. 2002. Identification and phylogeny of the human endogenous retorovirus HERV-W LTR family in human brain cDNA library and Xq21.3 region. J. Microbiol. Biotechnol. 12: 508-513
16 Kim, Y. T. and C. C. Richardson. 1994. Acidic carboxylterminal domain of gene 2.5 protein of bacteriophage T7 is essential for protein-protein interactions. J. Biol. Chem. 269: 5270-5278
17 Kim, Y. T., Y. H. Song, and J. E. Churchich. 1997. Recombinant brain 4-aminobutyrate aminotransferases: Overexpression, purification, and identification of Lys-330 at the active site. Biochim. Biophys. Acta 1337: 248-256   DOI   ScienceOn
18 Kossel, A. H., S. B. Cambridge, U. Wagner, and T. Bonhoeffer. 2001. A caged Ab reveals an immediate/instructive effect of BDNF during hippocampal synaptic potentiation. Proc. Natl. Acad. Sci. USA 98: 14702- 14707
19 Eide, F. F., D. H. Lowenstein, and L. F. Reichardt. 1993. Neurotrophins and their receptors - current concepts and implications for neurologic disease. Exp. Neurol. 121: 200-214   DOI   ScienceOn
20 Carter, B. D., U. Zirrgiebel, and Y. A. Barde. 1995. Differential regulation of $p21^{ras}$ activation in neurons by nerve growth factor and brain-derived neurotrophic factor. J. Biol. Chem. 270: 21751-21757   DOI   ScienceOn
21 Sendtner, M., B. Holtmann, R. Kolbeck, H. Thoene, and Y. A. Barde. 1992. Brain-derived neurotrophic factor prevents the death of motoneurons in newborn rats after nerve section. Nature 360: 757-759   DOI   ScienceOn
22 Leibrock, J., F. Lottspeich, A. Hohn, M. Hofer, B. Hengerer, P. Masiakowski, H. Thoenen, and Y. A. Barde. 1989. Molecular cloning and expression of brain-derived neurotrophic factor. Nature 341: 149-152   DOI   ScienceOn
23 Nilsson, A. S., M. Fainzilber, P. Falck, and C. F. lbafiez, 1998. Neurotrophin-7: A novel member of the neurotrophin family from the zebrafish. FEBS Lett. 424: 285-290   DOI   ScienceOn
24 Wilson, H. and L. C. Katz. 2002. BDNF release from single cells elicits local dendritic growth in nearby neurons. Nat. Neurosci. 5: 1177-1184   DOI   ScienceOn
25 Aloe, L., M. D. Simone, and F. Properzi. 1999. Nerve growth factor: A neurotrophin with activity on cells of the immune system. Microsc. Res. Tech. 45: 285-291   DOI   ScienceOn
26 Kim, Y. T. and C. C. Richardson. 1993. Bacteriophage T7 gene 2.5 protein: An essential protein for DNA replication. Proc. Natl. Acad. Sci. USA 90: 10 173 - 10177
27 Cho, J. J., J. H. Lee, S.-K. Kim, T.-1. Choi, and Y. T. Kim. 1999. cDNA encoding nm23/NDP kinase gene from Korean tiger shark, Scyliorhinus torazame. Mar. Biotechnol. 1: 131-136   DOI
28 Kim, Y.-J., N.-R. Lee, S.-Y. Choi, and K.-H. Min. 2002. Molecular cloning of the nahC gene encoding 1,2-dihydroxynaphthalene dioxygenase from Pseudomonas jluorescens. J. Microbiol. Biotechnol. 12: 172-175
29 Rodriguez-Tebar, A. and Y. A. Barde. 1988. Binding characteristics of brain-derived neurotrophic factor to its receptors on neurons from the chick embryo. J. Neurosci. 8: 3337-3342   DOI
30 Lo, D. C. 1992. Signal transduction and regulation of neurotorphins. Curr. Opin. Neurobiol. 2: 336-340   DOI   PUBMED   ScienceOn
31 Lee, J. H., S. W. Nam, and Y. T. Kim. 2003. Isolation of cDNAs for gonadotrophin-II of flounder (Paralichthys olivaceus) and its expressions in adult tissues. J. Microbiol. Biotechnol. 13: 710-716
32 Lanave, C., M. Attimonelli, M. De Robertis, F. Licciulli, S. Liuni, E. Sbisa, and C. Saccone. 1999. Update of AMmtDB: A database of multi-aligned metazoan mitochondrial DNA sequences. Nucleic Acids Res. 27: 134-137   DOI   ScienceOn
33 Biffo, S., J. Verhaagen, L. H. Schrama, P. Schotman, W. Danho, and F. L. Margolis. 1990. B-50/GAP43 expression correlates with process outgrowth in the embryonic mouse nervous system. Eur. J. Neurosci. 2: 487-499   DOI   ScienceOn
34 Heinrich, G 2003. A novel BDNF gene promoter directs expression to skeletal muscle. BMC Neurosci. 4: 11   DOI   PUBMED   ScienceOn
35 Van de Peer, Y. and R. De Wachter. 1994. TREECON for Windows: A software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput. Appl. Biosci. 10: 569-570
36 Lim, J. M., Y.-H. lang, H.-R. Kim, Y. T. Kim, T. J. Choi, J. Kim, and S. W. Nam. 2004. Overexpression of arylsulfatase in E. coli and its application to desulfatatioh of agar. J. Microbiol. Biotechnol. 14: 777-782
37 Maisonpierre, P. C., L. Belluscio, J. C. Conover, and O. D. Yancopoulos. 1992. Gene sequences of chicken BDNF and NT-3. DNA Seq. 3: 49-54   DOI   ScienceOn
38 Murphy,W. J., E. Eizirik, W. E. Johnson, Y. P. Zhang, O. A. Ryder, and S. J. O'Brien. 2001. Molecular phylogenetics and the origins of placental mammals. Nature 409: 614-618
39 Alderson, R. F., A. L. Alterman, Y. A. Barde, and R. M. Lindsay. 1990. Brain-derived neurotrophic factor increases survival and differentiated functions of rat septal cholinergic neurons in culture. Neuron 5: 297-306   DOI   ScienceOn
40 Gotz, R., F. Raulf, and M. Schartl. 1992. Brain-derived neurotrophic factor is more highly conserved in structure and function than nerve growth factor during vertebrate evolution. J. Neurochem. 59: 432-442   DOI   PUBMED
41 Yu, T. S., S.-H. Yea, and H. S. Kim. 2004. A new species of hyphomycetes, Aspergillus coreanus sp. nov., isolated from traditional Korean Nuruk. J. Microbiol. Biotechnol. 14: 182-187
42 Davies, A. M., H. Thoenen, and Y. A. Barde. 1986. The response of chick sensory neurons to brain-derived neurotrophic factor. J. Neurosci. 6: 1897-1904   DOI
43 Dawson, H. N., R. Burlingame, and A. C. Cannons. 1997. Stable transformation of Chlorella: Rescue of nitrate reductase-deficient mutants with the nitrate reductase gene. Curr. Microbiol. 35: 356-362   DOI   ScienceOn
44 Hyman, C., M. Hofer, Y. A. Barde, M. Juhasz, G D. Yancopoulos, S. P. Squinto, and R. M. Lindsay. 1991. BDNF is a neurotrophic factor for dopaminergic neurons of the substantia nigra. Nature 350: 230-232   DOI   ScienceOn
45 Murer, M. G., Q. Van, and R. Raisman-Vozari. 2001. Brainderived neurotrophic factor in the control human brain, and in the Alzheimer's disease and Parkinson's disease. Prog. Neurobiol. 63: 71-124   DOI   ScienceOn
46 Lai, K. O., W. Y. Fu, F. C. Ip, and N. Y. Ip. 1998. Cloning and expression of a novel neurotrophin, NT-7, from carp. Mol. Cell. Neurosci. 11: 64-76   DOI   ScienceOn
47 Zuccato, C., A. Ciammola, D. Rigamonti, B. R. Leavitt, D. Goffredo, L. Conti, M. E. Macdonald, R. M. Friedlander, V. Silani, M. R. Hayden, T. Timmusk, S. Sipione, and E. Cattaneo. 2001. Loss of Huntington-mediated BDNF gene transcription in Huntington's disease. Science 293: 493-498   DOI   PUBMED   ScienceOn
48 Herzog, K. H., K. Bailey, and Y. A. Barde. 1994. Expression of the BDNF gene in the developing visual system of the chick. Development 120: 1643-1649
49 Kim, D. H., Y. T. Kim, J. J. Cho, J. H. Bae, S. B. Hur, I. Hwang, and T. J. Choi. 2002. Stable integration and functional expression of flounder growth hormone gene in transformed microalga, Chlorella ellipsoidea. Mar. Biotechnol. 4: 63-73   DOI