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Safety Assessment of Lactobacillus fermentum PL9005, a Potential Probiotic Lactic Acid Bacterium, in Mice  

PARK JONG-HWAN (Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
LEE YEONHEE (Department of Biology, Seoul Women's University)
MOON ENPYO (Department of Biology, A-jou University)
SEOK SEUNG-HYEOK (Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
BAEK MIN-WON (Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
LEE HUI-YOUNG (Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
KIM DONG-JAE (Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
KIM CHANG-HWAN (Toxicology Center, LG Life Sciences)
PARK JAE-HAK (Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.15, no.3, 2005 , pp. 603-608 More about this Journal
Abstract
We recently isolated a novel probiotic strain, Lactobacillus fermentum PL9005 (KCCM-10250), from infant feces and showed that it had a potential immunoenhancing effect. In the present study, a safety assessment of the bacteria was performed using a BALB/c mouse model. Mice were administered with L. fermentum PL9005 daily for 28 days. There were no detectable changes in body weight, feed intake, or clinical signs, and no significant difference in hematological parameters or blood biochemistry between the L. fermentum PL9005-fed and control groups. Bacterial translocation was detected in the mesenteric lymph nodes, liver, and spleen of some mice with and without L. fermentum PL9005 feeding, however, the organisms were not related to ingestion of L. fermentum PL9005; this was confirmed by PCR using a species-specific primer. No gross lesions were detected in the liver, spleen, or intestine of L. fermentum PL9005-fed or control mice. Mucosal thickness in the ileum, cecum, and colon of L. fermentum PL9005-fed mice was not significantly different from that of corresponding organs in control mice. No inflammation or epithelial cell degeneration in the intestines was observed in any mice. These results indicate that ingestion of L. fermentum PL9005 is safe in mice and can be applied in the functional food market.
Keywords
Lactobacillus fermentum PL9005; immunoenhancing effect; safety assessment; mice;
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1 Aguirre, M. and M. D. Collins. 1993. Lactic acid bacteria and human clinical infection. J. Appl. Bacteriol. 75: 95-107   DOI
2 Berg, R. D. 1983. Translocation of indigenous bacteria from the intestinal tract, pp. 333-352. In R. J. Hentges (ed.). Human Intestinal Microflora in Health and Disease. Academic Press, London
3 Boyd, H. B. 1993. Safety assessment of novel foods. Intern. Food Ingred. 5: 9-11
4 Hale, P. and A. Hill. 1973. The recovery of Lactobacillus sp. from the livers of healthy mice. Lab. Anim. 7: 119-124   DOI   ScienceOn
5 Holzapfel, W. H., P. Haberer, J. Snel, U. Schillinger, and J. H. J. Huis in't Veld. 1998. Overview of gut flora and probiotics. Int. J. Food Microbiol. 41: 85-101   DOI   ScienceOn
6 Kim, S. M., K. H. Lee, N. K. Lee, C. J. Kim, C. H. Kim, and H. D. Park. 2004. Antagonistic activity of polyfermenticin SCD against Helicobacter pylori KCTC 2948. J. Microbiol. Biotechnol. 14: 148-152
7 Klein, G., A. Pack, C. Bonaparte, and G. Reuter. 1998. Taxonomy and physiology of probiotic lactic acid bacteria. Int. J. Food Microbiol. 41: 103-125   DOI   ScienceOn
8 Willem, M., R. Fonden, U. Svensson, M. Saxelin, L. Morelli, K. Collins, D. Brassard, E. Shiffrin, G. Mogensen, B. Grenov, S. Salminen, E. Isolauri, A. von Wright, and T. M. Sandholm. 1997. Recommendations for safety of probiotic cultures for traditional or novel foods. Demonst. Nutr. Functional. Probio. Food Newslett. 2: 2
9 Fukushima, M. and M. Nakano. 1996. Effects of a mixture of organisms, Lactobacillus acidophilus or Streptococcus faecalis on cholesterol metabolism in rats fed on a fat- and cholesterol-enriched diet. Brit. J. Nutr. 76: 857-867   DOI   ScienceOn
10 Zhou, J. S., Q. Shu, K. J. Rutherfurd, J. Prasad, M. J. Birtles, P. K. Gopal, and H. S. Gill. 2001. Safety assessment of potential probiotic lactic acid bacterial strains Lactobacillus rhamnosus HN001, Lb. acidophilus HN017, and Bifidobacterium lactis HN019 in BALB/c mice. Int. J. Food Microbiol. 56: 87-96
11 Park, J. H., Y. Lee, E. Moon, S. H. Seok, S. A. Cho, M. W. Baek, H. Y. Lee, D. J. Kim, and J. H. Park. 2005. Immunoenhancing effects of a new probiotic strain, Lactobaeillus fermentum PL 9005. J. Food. Prot. 68: 571-576   DOI
12 Walter, J., G. W. Tannock, A. Tilsala- Timisjarvi, S. Rodtong, D. M. Loach, K. Munro, and T. Alatossava. 2000. Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and speciesspecific PCR primers. Appl. Environ. Microbiol. 66: 297-303   DOI   ScienceOn
13 Ward, L. J. H., J. C. S. Brown, and G. P. Davey. 1995. Detection of dairy Leuconostoc strains using the polymerase chain reaction. Lett. Appl. Microbiol. 20: 204-208   DOI   ScienceOn
14 Ford, H. R., A. Avanoglu, P. R. Boechat, R. Melgoza, R. S. LumCheong, P. Boyle, M. Garrett, and M. I. Rowe. 1996. The microenvironment influences the pattern of bacterial translocation in formula-fed neonates. J. Pediat. Surg. 31: 486-489   DOI   ScienceOn
15 Fuller, R. 1991. Probiotics in man and animals. J. Appl. Bacteriol. 66: 365-378
16 Gill, H. S., K. J. Rutherfurd, J. Prasad, and P. K. Gopal. 2000. Enhancement of natural and acquired immunity by Lactobacillus rhamnosus (HN001), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019). Brit. J. Nutr. 83: 167-176   DOI   ScienceOn
17 Marteau, P. and J. C. Rambaud. 1993. Potential of using lactic acid bacteria for therapy and immunomodulation in man. Microbiol. Rev. 12: 207-220
18 De Rodas, B. Z., S. E. Gilliland, and C. V. Maxwell. 1996. Hypocholesterolemic action of Lactobacillus acidophilus ATCC 43121 and calcium in swine with hypercholesterolemia induced by diet. J. Dairy Sci. 79: 2121-2128   DOI   PUBMED   ScienceOn
19 Fuller, R. 1992. History and development of probiotics, pp. 1-8. In Fuller, R. (ed.), Probiotics - The Scientific Basis. Chapman and Hall, London
20 Hull, R. 1995. Probiotics and human health. Proc. Nutr. Soc. Aust. 19: 88-93
21 Tortuero, F., J. Rioperez, E. Fernandez, and M. L. Rodriguz. 1995. Response of piglets to oral administration of lactic acid bacteria. J. Food Prot. 58: 1369-1374   DOI
22 Zhou, J. S., Q. Shu, K. J. Rutherfurd, J. Prasad, P. K. Gopal, and H. S. Gill. 2000. Acute oral toxicity and bacterial translocation studies on potentially probiotic strains of lactic acid bacteria. Food Chem. Toxicol. 38: 153-161   DOI   ScienceOn
23 Lee, H. Y., J. H. Park, S. H. Seok, S. A. Cho, M. W. Baek, D. J. Kim, Y. H. Lee, and J. H. Park. 2004. Dietary intake of various lactic-acid bacteria suppresses type 2 helper T cell-production in antigen-primed mice splenocyte. J. Microbiol. Biotechnol. 14: 167-170
24 Kim, H. J., J. H. Kim, J. H. Son, H. J. Seo, S. J. Park, N. S. Paek, and S. K. Kim. 2004. Characterization of bacteriocin produced by Lactobacillus bulgaricus. J. Microbiol. Biotechnol. 14: 503-508
25 Matar, C., J. C. Valdez, M. Medina, M. Rachid, and G. Perdigon. 2001. Immunomodulating effects of milks fermented by Lactobacillus helveticus and its non-proteolytic variant. J. Dairy Res. 68: 601-609
26 Swendseid, M. E. 1987. Biochemical assessment of protein and amino acid status, pp. 205-211. In Livingston, G. E. (ed.), Proceedings of a National Conference Sponsored by the Food and Nutrition Council of the American Health Foundation, Nutritional Status Assessment of the Individual. Food and Nutrition Press, New York, U.S.A
27 Donohue, D. C., S. Salminen, and P. Marteau. 1998. Safety of probiotic bacteria, pp. 307. In S. Salminen and A. von Wright (eds.) Lactic Acid Bacteria. Marcel Dekker, New York, U.S.A
28 Gill, H. S., Q. Shu, H. Lin, K. J. Rutherfurd, and M. L. Cross. 2001. Protection against trans locating Salmonella typhimurium infection in mice by feeding the immunoenhancing probiotic Lactobacillus rhamnosus strain HN001. Med. Microbiol. Immunol. 190: 97-104
29 Gasser, F. 1994. Safety of lactic acid bacteria and their occurrence in human clinical infections. Bull. Pasteur Instit. 92: 45-67
30 Kato, J., K. Endo, and S. Rybka. 1997. Effects of oral administration of Lactobacillus casei on antitumor responses induced by tumor resection in mice. Int. J. Immunopharmacol. 16: 29-36   DOI   ScienceOn
31 Ma, L., E. Deitch, R. Specian, E. Steffen, and R. Berg. 1990. Translocation of Lactobacillus murinus from the gastrointestinal tract. Curr. Microbiol. 20: 177-184   DOI
32 Gordon, L. E., D. Ruml, H. J. Hahne, and C. P. Miller. 1995. Studies on susceptibility to infection following radiation. IV: The pathogenesis of endogenous bacteremia of mice. J. Exp. Med. 102: 413-424   DOI   ScienceOn
33 Kim, S. H., S. J. Yang, H. C. Koo, W. K. Bae, J. Y. Kim, J. H. Park, Y. J. Baek, and Y. H. Park. 2001. Inhibitory activity of Bifidobacteria longum HY8001 against vero cytotoxin of Escherichia coli O157:H7. J. Food Prot. 64: 1667-1673   DOI
34 Shu, Q., K. J. Rutherfurd, S. G. Fenwick, J. Prasad, P. K. Gopal, and H. S. Gill. 2000. Dietary Bifidobacterium lactis (HN019) enhances resistance to oral Salmonella typhimurium infection in mice. Microbiol. Immunol. 44: 213-222   DOI
35 Perdigon, G., M. E. N. de Marcias, S. Alvarez, G. Oliver, and A. P. de Ruiz Holgado. 1990. Prevention of gastrointestinal infection using immunobiological methods with milk fermented with Lactobacillus casei and Lactobacillus acidophilus. J. Dairy Res. 57: 255-264   DOI   ScienceOn
36 Bayer, A. S., A. W. Chow, D. Betts, and L. B. Guze. 1978. Lactobacillemia - report of nine cases, and important clinical and therapeutic considerations. Am. J. Med. 64: 808-813   DOI   ScienceOn
37 Matsuzaki, T. 1998. Immunomodulation by treatment with Lactobacillus casei strain Shirota. Int. J. Food Microbiol. 41: 133-140   DOI   PUBMED   ScienceOn
38 van der Kamp, J. W. 1996. Safety considerations regarding probiotic strains - conclusions of two specialist workshops. Int. Dairy Feder. Newslett. 5: 27 - 28