Browse > Article

Antiplatelet Activity of Thujopsis dolabrata var. hondai-Derived Component Against Platelet Aggregation  

SON DONG JU (College of Natural Sciences, Soonchunhyang University)
PARK YOUNG HYUN (College of Natural Sciences, Soonchunhyang University)
KIM YOUNG MI (Research Center for Industrial Development of Biofood Materials and Faculty of Biotechnology, College of Agriculture, Chonbuk National University)
CHUNG NAM HYUN (College of Life and Environmental Sciences, Korea University)
LEE HOI SEON (Research Center for Industrial Development of Biofood Materials and Faculty of Biotechnology, College of Agriculture, Chonbuk National University)
Publication Information
Journal of Microbiology and Biotechnology / v.15, no.2, 2005 , pp. 425-427 More about this Journal
Abstract
The steam distillate obtained from Thujopsis dolabrata var. hondai sawdust was fractionated by centrifugal thin-film evaporation, and the fractions were then investigated for antiplatelet activity using washed rabbit platelets. The biologically active constituent of T. dolabrata var. hondai sawdust was isolated by silica gel column and HPLC chromatographies and characterized as carvacrol by various spectral analyses. Carvacrol inhibited platelet aggregation induced by collagen, arachidonic acid, and platelet activating factor with IC$_{50}$ values of 12.6, 2.5, and 385.3 $\mu$M, respectively. However, carvacrol had no effect on thrombin, calcium ionophore A23l87, or phorbol l2-myristate l3-acetate induced platelet aggregation. Carvacrol was a much more potent inhibitor, as antiplatelet agents, compared with aspirin. These results suggest that carvacrol isolated from T. dolabrata var. hondai sawdust may be useful as a lead compound for inhibiting arachidonic acid-induced platelet aggregation.
Keywords
Citations & Related Records

Times Cited By Web Of Science : 7  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Balandrin, M., J. Klocke, E. S. Wurtele, and W. H. Bollinger. 1985. Natural plant chemicals: Sources of industrial and medicinal materials. Science 228: 1154- 1160   DOI   PUBMED
2 Hirsh. J. 1987. Hyperactive platelets and complications of coronary artery disease. N. Engl. J. Med. 316: 1543- 1544   DOI   PUBMED   ScienceOn
3 Ito. M., M. Hamada. M. Arakawa, and F. Abe. 1980. Antimicrobiol activities of Hiba oil, Thujopsene and its various derivatives. Bokin Bobai 8: 3- 6
4 Packham, M. A. 1994. Role of platelets in thrombosis and hemostasis. Can. J. Physiol. Pharm. 72: 278- 284   DOI   ScienceOn
5 Samuelsson, B., M. Goldyne, E. Granstrom, M. Hamberg. S. Hammarstrom, and C. Malmsten. 1978. Prostaglandins and thromboxanes, Annu. Rev. Biochem. 47: 997- 1029   DOI   ScienceOn
6 Enomoto, H., Y. Yoshikuni, Y. Yasutomi, K. Ohata, K. Sempuku, K. Kitaguchi, Y. Fujita, and T. Mori. 1997. Hypocholesterolemic action of tricyclic diterpenoids in rats. Chem. Pharm. Bull. 25: 507 - 510
7 Chang, F. R., J. L. Wei, C. M. Teng, and Y. C. Wu. 1998. Antiplatelet aggregation constituents from Annona purpurea. J. Nat. Prod. 61: 1457-1461   DOI   ScienceOn
8 Kim. M. K. and H. S. Lee. 2002. Growth responses of various flower extracts toward human intestinal microflora. Food Sci. Biotechnol. 11: 444-447
9 Tsai. I. L., W. Y. Lin, C. M. Teng, T. Ishikawa. S. L. Doong, M. W. Huang, Y. C. Chen, and I. S. Chen. 2000. Coumarins and antiplatelet constituents from the root bark of Zanthoxylum schinifolium. Planta Medica 66: 618- 723   DOI   ScienceOn
10 Hwang. Y. H. and H. S. Lee. 2002. Antibacterial activity of Pinus densijlora leaf-derived components toward human intestinal bacteria. J. Microbiol. Biotechnol. 12: 610- 616
11 Song, M. H., K. Sclvam, H. Y. Jeong. and K. S. Chae. 2003. Inhibition of asexual sporulation and growth of Aspergillus niger and Aspergillus oryzae by propylamine. J. Microbiol. Biotechnol. 13: 146- 148
12 Son. D. J., S. E. Lee. and B. S. Park. 2003. Inhibitory effects of naturally occurring flavonoids on human intestinal bacterium. Clostridium perfringens. Food Sci. Biotechnol. 12: 180- 182
13 Dinerman, J. L. and J. L. Mehta. 1990. Endothelial, platelet and leukocyte interactions in ischemic heart disease: Insights into potential mechanisms and their clinical relevance. J. Am. Cardiol. 16: 207- 222   DOI
14 Ahn, Y. J., S. B. Lee, T. Okubo, and M. Kim. 1995. Antignawing factor of crude oil derived from Thujopsis dolabrata S. et. Z. var. hondai sawdust against mice. J. Chemical Ecology 21: 263- 271   DOI   ScienceOn
15 Ross. R. 1986. The pathogenesis of atherosclerosis: An update. N. Engl. J. Med. 314: 488- 500   DOI   PUBMED   ScienceOn
16 Siess, W. 1989. Molecular mechanisms of platelet activation. Physiol. Rev. 69: 58- 178   DOI   PUBMED
17 Ahn, Y. J., S. B. Lee, H. S. Lee, and G. H. Kim. 1998. Insecticidal and acaricidal activity of carvacrol and $\beta-thujaplicine$ derived from Thujopsis dolabrata var, hondai sawdust. J. Chemical Ecology 24: 81- 90   DOI   ScienceOn
18 Rho. M. C., Y. H. Park. S. Sasaki. M. Ishibashi. K. Kondo. J. Kobayashi. and Y. Ohizumi. 1996. The mode of rabbit platelet shape change and aggregation induced by theonezolide-A. a novel polyketidc macrolide, isolated from the Okinawan marine sponge Theonella sp. Can. J. Physiol. Pharm. 74: 193- 199   DOI   ScienceOn
19 Singh. D. P., A. Kumar. and M. B. Tyagi. 2003. Biotoxic cyanobacterial metabolites exhibiting pesticidal and mosquito larvicidal activities, J. Microbiol. Biotechnol. 13: 50- 56
20 Hasegawa. S. and Y. Hirose. 1982. Terpenoids from the seed of Thujopsis dolabrata var. dolabrata. Phytochemisty 21: 643- 646   DOI   ScienceOn
21 Tsai, W. J., W. C. Hsin, and C. C. Chen. 1996. Antiplatelet flavonoids from seeds of Psoralea corylifolia. J. Nat. Prod. 59: 671-672   DOI   ScienceOn
22 Lin. C. N. and C. M. Lu. 1996. Novel antiplatelet constituents from Formosan Moraceous plants. J. Nat. Prod. 59: 834- 838   DOI   ScienceOn