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Electrochemical Regeneration of FAD by Catalytic Electrode Without Electron Mediator and Biochemical Reducing Power  

JEON SUNG JIN (Department of Biological Engineering, Seokyeong University)
SHIN IN HO (Department of Biological Engineering, Seokyeong University)
SANG BYUNG IN (Division of Water Environment and Remediation, KIST)
PARK DOO HYUN (Department of Biological Engineering, Seokyeong University)
Publication Information
Journal of Microbiology and Biotechnology / v.15, no.2, 2005 , pp. 281-286 More about this Journal
Abstract
We created a new graphite-Cu(II) electrode and found that the electrode could catalyze FADH$_2$ oxidation and FAD reduction coupled to electricity production and consumption, respectively. In a fuel cell with graphite-Cu(II) anode and graphite-Fe(III) cathode, the electricity was produced by coupling to the spontaneous oxidation of FADH$_2$ Fumarate and xylose were not produced from the enzymatic oxidation of succinate and xylitol without FAD, respectively, but produced with FAD. The production of fumarate and xylose in the reactor with FAD electrochemically regenerated was maximally 2- 5 times higher than that in the reactor with FAD. By using this new electrode with catalytic function, a bioelectrocatalysts can be engineered; namely, oxidoreductase (e.g., lactate dehydrogenase) and FAD can function for biotransformation without an electron mediator and second oxidoreductase for cofactors recycling.
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