Browse > Article

Screening and Biotransformation of Interleukin-1$\beta$ Converting Enzyme Production Inhibitors from Arctii fructus  

KIM HYUN A (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology)
YOON DO YOUNG (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology)
LEE SANG MYUNG (KT & G Central Research)
BAEK SEUNG HWA (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology)
HAN GYOON HEE (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology)
KHO YOUNG HEE (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology)
LEE CHOONG HWAN (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Journal of Microbiology and Biotechnology / v.15, no.2, 2005 , pp. 269-273 More about this Journal
Abstract
Five dibenzylbutyrolactones were isolated from a methanol extract of Arctii fructus (Arctium lappa L.) by bioassay-guided isolation, using the interleukin-l $\beta$ converting enzyme (caspase-l, ICE) production inhibitory assay in vitro. These compounds were spectroscopically identified as lappaol E (1), lappaol A (2), matairesinol (3), arctigenin (4), and arctiin (5). Among the compounds tested, arctigenin (4) showed the strongest inhibitory activity for ICE production in IL-$\beta$-induced proliferation of D 1 OS cells. Western blot analysis demonstrated that the arctigenin suppressed the expression of ICE protein in a dose-dependent manner. To estimate the biotransformation of Arctii fructus in vivo by human intestinal bacteria, we carried out an anaerobic incubation of the Arctii fructus extract with a human fecal suspension. From the HPLC analysis of metabolites, Arctiin (IC$_{50}$=74.2$\mu$g/ml), a major component of Arctii fructus, was transformed to aglycone, arctigenin (IC$_{50}$=12.5$\mu$g/ml), by human intestinal bacteria. The ICE production inhibitory activity of Arctii fructus would be much stronger in vivo than in vitro due to the biotransformation by human intestinal bacteria.
Keywords
Citations & Related Records

Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Alnemri, E. S., D. J. Livingston, D. W. Nicholson, G. Salvesen, N. A. Thornberry, W. W. Wong, and J. Yuan. 1996. Human ICE/CED-3 protease nomenclature. Cell 87: 171   DOI   ScienceOn
2 Estevez-Brann, A., R. Estevez-Reyes, and G. Gonzalez. 1996. $^{13}C$ NMR assignments of some $dibenzyl-\gamma -butyrolactone$ Iignans. Phytochemistry 43: 885- 886   DOI   ScienceOn
3 Goodwin, C. J., S. J. Holt, S. Downes, and N. J. Marshall. 1995. Microculture tetrazolium assays: A comparison between two new tetrazolium salts, XTT and MTS. J. lmmunol. Methods 179: 95- 103   DOI   ScienceOn
4 Song, J.-S., S.-B. Kim, Y.-H. Lee, K.- W. Lee, H.-H. Jung, M.-H. Kim, K.-T. Kim, R. Brown, and Y.-T. Kim. 2002. Adenovirus-mediated antisense expression of telomerase template RNA induces apoptosis in lung cancer cells. J. Microbiol. Biotechnol. 12: 89- 95
5 Yoon, D. Y. and C. A. Dinarello. 1998. Antibodies to domains II and III of the IL-1 receptor accessory protein inhibit IL-1 activity but not binding: Regulation of IL-1 responses is via type I receptor, not the accessory protein. J. Immunol. 160: 3170- 3179   PUBMED
6 Bae, K. H. 2000. The medical plants Korea 1996. p. 487
7 Dinarello, C. A. 1998. Interieukin-1 beta, interleukin-18, and interleukin-1 beta converting enzyme. Ann. N. Y. Acad. Sci. 856: 1- 11   DOI   PUBMED
8 Thornberry, N. Y. and M. M. Susan. 1995. $Interleukin-1\beta$ converting enzyme: A novel cysteine protease required for $IL-1\beta$ production and implicated in programmed cell death. Protein Science 4: 3- 12   PUBMED
9 Tiwari, A. K., P. V. Srinivas, S. P. Kumar, and J. M. Rao. 2001. Free radical scavenging active components from Cedrus deoeara. J. Agric. Food Chem. 49: 4642-4645   DOI   ScienceOn
10 Jeong, Y.-W, K.-S. Kim, J.-Y. Oh, J.-C. Park, J.-H. Bang, S.-W. Choi, and J.-C. Lee. 2003. Growth inhibition and apoptosis induction of gastric cancer cells by copper (II) glycinate complex. J. Microbiol. Biotechnol. 13: 394- 399
11 Orencole, S. F. and C. A. Dinarello. 1989. Characterization of a subclone (D1 0S) of the D10.G4.1 helper T-cell line which proliferates to attomolar concentrations of interleukin1 in the absence of mitogens. Cytokine 1: 14- 22   DOI   ScienceOn
12 Park, H.-W., D.-Y. Jun, and Y.-H. Kim. 2002. Apoptotic activity of insect pathogenic fungus Paecilomyces japonica toward human acute leukemia jurkat T cells is associated with mitochondria-dependent caspase-3 activation regulated by Bcl-2. J. Microbiol. Biotechnol.12: 950- 956
13 Shuiji, O., B. D. Laurence, and G. L. Norman. 1993. Formation of (-)-Arctigenin in Forsythia intermedia. Phytochemistry 32: 643- 652   DOI   ScienceOn
14 Tundis, R., G. Statti, F. Menichini, and F. D. Monache. 2000. Arctiin and onopordopicrin from Carduus micropterus ssp. Perspinosus Fitoterapia 71: 600- 601   DOI   ScienceOn
15 Wang, L. Q., M. R. Meselhy, Y. Li, G. W. Qin, and M. Hattori. 2000. Human intestinal bacteria capable of transforming secoisolariciresinol diglucoside to mammalian lignans, enterodiol and enterolactone. Chem. Pharm. Bull. 48: 1606- 1610   DOI   PUBMED   ScienceOn
16 Guo, Y. and N. R. Kyprianou. 1999. Restoration of transforming growth $factor-\beta$ signaling pathway in human protease cancer cells suppresses tumorgenicity via induction of caspase-1 mediated apoptosis. Cancer Res. 59: 1366-1371   PUBMED
17 Gurtu, V. S., R. Kain, and G. Zhang. 1997. Fluorometric and colorimetric detection of caspase activity associated with apoptosis. Anal. Biochem. 251: 98- 102   DOI   ScienceOn
18 Ichihara, A., Y. Numata, K. Seiji, and S. Sakamura. 1977. New sesquilignan from Arctium lappa L. The structure of lappaol C, D and E. Agric. Biol. Chem. 41: 1813- 1814   DOI
19 Miller, D. K., J. R. Calaycay, K. T. Chapman, A. D. Howard, M. J. Kostura, S. M. Molineaux, and N. A. Thornberry. 1993. The IL-1 beta converting enzyme as a therapeutic target. Ann. N. Y. Acad. Sci. 696: 133- 148   PUBMED
20 Thornberry, N. Y., H. G. Bull, J. R Calaycay, K. T. Chapman, A. D. Howard, M. J. Kostura, D. K. Miller, S. M. Molineaux, and J. R. Toyokuni, et al. 1992. A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes. Nature 356: 768- 777   DOI   ScienceOn
21 Ichihara, A., S. Kanai, Y. Nakamura, and S. Sakamura. 1976. Lappaol A and B, novel lignans from Arctium lappa L. Tetrahedron Lett. 44: 3961- 3964
22 Kim, N.-S., K.-H. Chang, B.-S. Chung, S.-H. Kim, J.-H. Kim, and G.-M. Lee. 2003. Characterization of humanized antibody produced by apoptosis-resistant CHO cells under sodium butyrate-induced condition. J. Microbiol. Biotechnol. 13: 926- 936
23 Umehara, K., A. Sugawa, M. Kuroyanagi, A. Ueno, and T. Taki. 1993. Studies on differentiation-inducers from Arctium fructus. Chem. Pharm. Bull. 41: 1774- 1779   DOI   ScienceOn
24 Dinarello, C. A. 1996. Biological basis for interleukin-1 disease. Blood 87: 2095- 2147   PUBMED