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Optimization of Chitosan-Alginate Encapsulation Process Using Pig Hepatocytes or Development of Bioartificial Liver  

LEE , JI-HYUN (Samsung Biomedical Research Institute)
LEE, DOO-HOON (Department of Chemical Engineering, Dongguk University)
SON, JEONG-HWA (Department of Biotechnology and Bioengineering, Pukyong National University)
PARK, JUNG-KEUG (Department of Chemical Engineering, Dongguk University)
KIM, SUNG-KOO (Department of Biotechnology and Bioengineering, Pukyong National University)
Publication Information
Journal of Microbiology and Biotechnology / v.15, no.1, 2005 , pp. 7-13 More about this Journal
Abstract
Chitosan-alginate capsules were formed by electrostatic interactions and exhibited an appropriate mechanical strength, permeability, and stability for the culture of hepatocytes. Pig hepatocytes were isolated and hepatocyte spheroids formed and immobilized in chitosan-alginate capsules. An encapsulation procedure of 3 min and spheroid formation period of 24 h were the optimum conditions for the best liver functions. Pig hepatocytes with a cell density of $6.0{\tomes}10^6$ cells/ml in the capsules were found to be most suitable for application in a bioartificial liver support system. The encapsulated pig hepatocyte spheroids exhibited stable ammonia removal and urea secretion rates in a bioreactor for 2 weeks. Accordingly, chitosan-alginate encapsulated hepatocyte spheroids in a packed-bed bioreactor would appear to have potential as a bioartificial liver.
Keywords
Encapsulation; alginate; chitosan; collagen; hepatocyte; bioartificial liver;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 17  (Related Records In Web of Science)
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