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Stimulation of Actinorhodin Production by Streptomyces lividans with a Chromosomally-Integrated Antibiotic Regulatory Gene afsR2  

Kim, Chang-Young (School of Chemical Engineering and Biotechnology, Inha University)
Park, Hyun-Joo (School of Chemical Engineering and Biotechnology, Inha University)
Yoon, Yeo-Joon (Division of Nano Sciences and Department of Chemistry, Ewha Woman's University)
Kang, Han-Young (Department of Chemistry, Chungbuk National University)
Kim, Eung-Soo (School of Chemical Engineering and Biotechnology, Inha University)
Publication Information
Journal of Microbiology and Biotechnology / v.14, no.5, 2004 , pp. 1089-1092 More about this Journal
Abstract
An actinorhodin nonproducing Streptomyces lividans was converted to an actinorhodin overproducer through a single chromosomal integration of an antibiotic regulatory gene, afsR2. This strain exhibited early actinorhodin production and an average of 37.5% higher productivity than the S. lividans containing multiple copies of afsR2 plasmid in a glucose-containing liquid culture.
Keywords
afsR2; chromosomal integration; actinorhodin; Streptomyces lividans;
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Times Cited By Web Of Science : 14  (Related Records In Web of Science)
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1 Bibb, M. J. 1996. The regulation of antibiotic production in Streptomyces coelicolor A3(2). Microbiology 142: 1335-1344.   DOI   PUBMED   ScienceOn
2 Hu, H., Q. Zhang, and K Ochi. 2002. Activation of antibiotic biosynthesis by specified mutation in the rpoB gene (encoding the RNA polymerase $\beta$ subunit) of Streptomyces lividans. J. Bacterioi. 184: 3984- 3991.
3 Jung, W.-S., E.-S. Kim, H.-Y Kang, C.-Y Choi, D. H. Sherman, and Y.-J. Yoon. 2003. Site-directed mutagenesis on putative macrolactone ring size determinant in the hybrid pikromycin-tylosin polyketide synthase. J. Microbiol. Biotechnol. 13(5): 823- 827.
4 Lee, J.-Y., Y-S. Hwang, S.-S. Kim, E.-S. Kim, and C.-Y Choi. 2000. Effect of a global regulatory gene, afsR2, from Streptomyces lividans on avermectin production in Streptomyces avermitilis. J. Biosci. Bioeng. 89: 606- 608.
5 Chater, K F. 2001. Genetics of differentiation in Streptomyces. Annu. Rev. Microbial. 47: 685-713.
6 Floriano, B. and M. J. Bibb. 1996. afsR is a pleiotropic but conditionally required regulatory gene for antibiotic production in Streptomyces coelicolor A3(2). Mol. Microbial. 21: 385-396.
7 Chater, K. F. and M. J. Bibb. 1997. Regulation of bacterial antibiotic production, pp. 59- 105. In Kleinkauf, H. and von Dohren, H. (eds.), Products of Secondary Metabolism, Biotechnology, 7. Weinheim, Germany: VCH.
8 Vogtli, M., P.-C. Chang, and S. N. Cohen. 1994. afsR2: A previously undetected gene encoding a 63-amino-acid protein that stimulates antibiotic production in Streptomyces lividans. Mol. Microbiol. 14: 643- 653.
9 Lee, P. C., T. Umeyama, and S. Horinouchi. 2002. afsS is a target of AfsR, a transcriptional factor with ATPase activity that globally controls secondary metabolism in Streptomyces coelicolor A3(2). Mol. Microbiol. 43: 1413-1430.
10 Sohng, J.-K, H.-R. Oh, O.-H. Lee, S.-J. Kim, J.-M. Han, S.-K Nam, and J.-C. Yoo. 2002. Function of lysine-148 in dTDPD- glucose 4,6-dehydratase from Streptomyces antibioticus Tu99. J. Microbial. Biotechnol. 12(2): 217- 221.
11 Umeyama, T., P.-C. Lee, and S. Horinouchi. 2002. Protein serine/threonine kinase in signal transduction for secondary metabolism and morphogenesis in Streptomyces. Appl. Microbial. Biotechnol. 59: 419- 425.   DOI   ScienceOn
12 Hopwood, D. A,M. J. Bibb, K F. Chater, T. Kieser, C. J. Bruton, H. M. Keiser, D. J. Lydiate, C. P. Smith, J. M. Ward, and H. Schrempf. 1985. Genetic Manipulation of Streptomyces: A Laboratory Manual, John Innes Foundation, Norwich, U.K.
13 Kim, E.-S., H.-J. Hong, C.-Y Choi, and S. N. Cohen. 2001. Modulation of actinorhodin biosynthesis in Streptomyces lividans by glucose repression of afsR2 gene transcription. J. Bacterial. 183: 2198- 2203.
14 Hesketh, A R., G. Chandra, A D. Shaw, J. J. Rowland, D. B. Kell, M. J. Bibb, and K. F.Chater. 2002. Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor. Mol. Microbial. 46: 917- 932.
15 Kim, C.-Y, H.-J. Park, and E.-S. Kim. 2003. Heterologous expression of hybrid type polyketide synthase system in Streptomyces species. J. Microbiol. Biotechnol. 13(5): 819-822.
16 Huang, J., C.-J. Lih, K.-H. Pan, and S. N. Cohen. 2001. Global analysis of growth phase responsive gene expression and regulation of antibiotic biosynthetic pathways in Streptomyces coelicolor using DNA microarrays. Genes Dev. 15: 3183-3192.
17 Vara, J., M. Lewandowska-Skarbek, Y. G. Wang, S. Donadio, and C. R. Hutchinson. 1989. Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythreus). J. Bacteriol. 171: 5872- 5881.
18 Katz, L. and S. Donadio. 1993. Polyketide synthesis: Prospects for hybrid antibiotics. Annu. Rev. Microbiol. 47: 875-912.