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Functional Complementation of Escherichia coli by the rpoS Gene of the Foodborne Pathogenic Vibrio vulnificus  

Park, Kyung-Je (Department of Environmental Science, Hankuk University of Foreign Studies)
Kim, Song-Hee (Department of Environmental Science, Hankuk University of Foreign Studies)
Kim, Min-Gon (Laboratory of Intergrative Biotechnology, Korea Research Institute of Bioscience and Biotechnology)
Chung, Duck-Hwa (Division of Applied Life Science, Gyeongsang National University)
Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University)
Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University)
Jahng, Deok-jin (Department of Environmental Engineering and Biotechnology, Myongji University)
Lee, Kyu-Ho (Department of Environmental Science, Hankuk University of Foreign Studies)
Publication Information
Journal of Microbiology and Biotechnology / v.14, no.5, 2004 , pp. 1063-1066 More about this Journal
Abstract
The rpoS gene product is a global transcriptional factor, which is involved in bacterial survival under various stress conditions. An rpoS-homologous gene was cloned from a septicemia-causing pathogenic Vibrio vulnificus. Introduction of this gene as a multicopy plasmid into various E. coli strains displayed functional complementation, for examples, increased survivability of an rpoS-defective E. coli cell and induction of known $\delta^S$-dependent, stress-responding promoters of E. coli genes.
Keywords
rpoS; functional complementation; foodborne pathogen;
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1 Clare, D. A., M. N. Duong, D. Darr, F Archibald, and I. Fridovich. 1984. Effects of molecular oxygen on detection of superoxide radical with nitroblue tetrazolium and on activity stains for catalase. Anal. Biochem. 140: 532- 537.
2 Hollis, D. G., R E. Weaver, C. N. Baker, and C. Thornsberry. 1976. Halophilic Vibrio species isolated from blood cultures. J. Clin. Microbiol. 3: 425- 431.
3 Lee, J. H., N. Y. Park, S.-J. Park, and S. H. Choi. 2003. Identification and characterization of the Vibrio vulnificus phosphomannomutase gene. J. Microbiol. Biotechnol. 13: 149- 154.
4 Miller, J. H. 1972. Experiments in Molecular Biology. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, U.S.A.
5 Yildiz, F H. and G. K Schoolnik. 1998. Role of rpoS in stress survival and virulence of Vibrio cholerae. J. Bacteriol. 180: 773- 784.
6 Lomovskaya, O. L., J. P. Kidwell, and A. Matin. 1994. Characterization of the a38-dependent expression of a core Escherichia coli starvation gene, pexB. J. Bacteriol. 176: 3928- 3935.
7 Matin, A. 1991. The molecular basis of carbon-starvationinduced general resistance in Escherichia coli. Mol. Microbiol. 5: 3-10
8 Park, K-J., M.-J. Kang, S. H. Kim, H.-J. Lee, J.-K Lim, S. H. Choi, S.-J. Park, and K-H. Lee. 2004. Isolation and characterization of rpoS in a pathogenic bacterium, Vibrio vulnificus: Role of as in survival of exponential-phase cells under oxidative stress. J. Bacteriol. 186: 3304- 3312.
9 Strom, M. S. and R N. Paranjpye. 2000. Epidemiology and pathogenesis of Vibrio vulnificus. Microbes Infect. 2: 177188.
10 Yanisch-Perron, c, J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103- 119
11 Kim, H. J., J. H. Lee, J. E. Rhee, H. S. Jeong, H. K Choi, H. J. Chung, S. Ryu, and S. H. Choi. 2002. Identification and functional analysis of the putAP genes encoding Vibrio vulnificus proline dehydrogenase and proline permease. J. Microbiol. Biotechnol. 12: 318- 326.
12 Bohannon, D. E., N. Connell, J. Keener, A. Tormo, M. Espinosa-Urgel, M. M. Zambrano, and R Kolter. 1991. Stationary-phase-inducible 'gearbox' promoters: Differential effects of katF mutations and role of $\sigma^{70}$. J. Bacteriol. 173: 4482-4492.   DOI
13 Mulvey, M. R, J. Switala, A. Borys, and P. C. Loewen. 1990. Regulation of transcription of katE and katF in Escherichia coli. J. Bacteriol. 172: 6713- 6720.
14 Schweder, T., K-H. Lee, O. Lomovskaya, and A. Matin. 1996. Regulation of Escherichia coli starvation sigma factor $(\sigma^S)$ by ClpXP protease. J. Bacteriol. 178: 470-476.
15 Hengge-Aronis, R 2002. Signal transduction and regulatory mechanisms involved in control of the $\sigma^S$(RpoS) subunit of RNA polymerase. Microbiol. Mol. BioI. Rev. 66: 373- 395.
16 Jeong, H. S., K C. Jeong, H. K Choi, K-J. Park, K-H. Lee, J. H. Rhee, and S. H Choi. 2001. Differential expression of Vibrio vulnificus elastase gene in a growth phase-dependent manner by two different types of promoters. J. Biol. Chem. 276: 13875- 13880.
17 Schellhorn, H. E. and V. L. Stones. 1992. Regulation of katF and katE in Escherichia coli K-12 by weak acids. J. Bacteriol. 174: 4769-4796.   DOI
18 McCann, M. P., J. P. Kidwell, and A. Matin. 1991. The putative factor KatF has a central role in development of starvation-mediated general resistance in Escherichia coli. J. Bacteriol. 173: 4188-4194.
19 Vivas, E. I. and H. Goodrich-Blair. 2001. Xenorhabdus nematophilus as a model for host-bacterium interactions: rpoS is necessary for mutualism with nematodes. J. Bacteriol. 183: 4687-4693.
20 Schultz, J. E., G. I. Latter, and A. Matin. 1988. Differential regulation by cyclic AMP of starvation protein synthesis in Escherichia coli. J. Bacteriol. 170: 3903- 3909.   DOI
21 Suh, S.-J., L. Silo-Suh, D. W. Woods, D. J. Hassett, S. E. H. West, and D. E. Ohman. 1999. Effect of rpoS mutation on the stress response and expression of virulence factors in Pseudomonas aeruginosa. J. Bacteriol. 181: 3890- 3897.
22 Jeong, H. S., J. E. Rhee, J. H. Lee, H. K Choi, D.-I. Kim, M. H. Lee, S.-J. Park, and S. H Choi. 2003. Identification of Vibrio vulnificus lip and its influence on survival under various stresses. J. Microbiol. Biotechnol. 13: 159- 163.
23 Burgess, R. Rand L. Anthony. 2001. How sigma docks to RNA polymerase and what sigma does. Curro Opin. Microbiol. 4: 126- 131.
24 Merrell, D. S., A. D. Tischler, S. H. Lee, and A. Camilli. 2000. Vibrio cholerae requires rpoS for efficient intestinal colonization. Infect. Immun. 68: 6691- 6696.
25 Ramos-Gonzalez, M. I. and S. Molin. 1998. Cloning, sequencing, and phenotypic characterization of the rpoS gene from Pseudomonas putida KT2440. J. Bacteriol. 180: 3421-3431.
26 Jorgensen, F, M. Bally, V. Chapon-Herve, G. Michel, A. Lazdunski, P Williams, and G. S. A. B. Stewart. 1999. RpoS-dependent stress tolerance in Pseudomonas aeruginosa. Microbiology 145: 835- 844.
27 McCann, M. P, C. D. Fraley, and A. Matin. 1993. The putative factor KatF is regulated posttranscriptionally during carbon starvation. J. Bacteriol. 175: 2143- 2149.